Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina

Autores
Fernandez, Franco Daniel; Galdeano, Ernestina; Conci, Luis Rogelio
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X) B. subalternans, H. ovatifolium, and C. maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B) and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-( B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
International Journal of Systematic and Evolutionary Microbiology 70 (1) : 35-43 (2020)
Materia
Phytoplasmas
Weeds
Phyllody
Witches' Broom
Classification
Filodia
Fitoplasmas
Malezas
Clasificación
Argentina
Phytoplasma Diversity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9668
Acceder
id INTADig_8eb01b27d284ea8c9d67478f666c998f
oai_identifier_str oai:localhost:20.500.12123/9668
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in ArgentinaFernandez, Franco DanielGaldeano, ErnestinaConci, Luis RogelioPhytoplasmasWeedsPhyllodyWitches' BroomClassificationFilodiaFitoplasmasMalezasClasificaciónArgentinaPhytoplasma DiversitySymptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X) B. subalternans, H. ovatifolium, and C. maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B) and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-( B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum.Instituto de Patología VegetalFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaMicrobiology Society2021-06-25T18:36:15Z2021-06-25T18:36:15Z2020-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9668https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.0037041466-50261466-5034 (online)https://doi.org/10.1099/ijsem.0.003704International Journal of Systematic and Evolutionary Microbiology 70 (1) : 35-43 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.info:eu-repograntAgreement/INTA/PNIND-1108071/AR./Estrategias de manejo de sistemas productivos resilientes.info:eu-repograntAgreement/INTA/PNIND-1108072/AR./Epidemiología de plagas y enfermedades en cultivos industriales con enfoque al desarrollo de estrategias de manejo integrado.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:23:47Zoai:localhost:20.500.12123/9668instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:23:48.327INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
title Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
spellingShingle Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
Fernandez, Franco Daniel
Phytoplasmas
Weeds
Phyllody
Witches' Broom
Classification
Filodia
Fitoplasmas
Malezas
Clasificación
Argentina
Phytoplasma Diversity
title_short Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
title_full Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
title_fullStr Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
title_full_unstemmed Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
title_sort Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
dc.creator.none.fl_str_mv Fernandez, Franco Daniel
Galdeano, Ernestina
Conci, Luis Rogelio
author Fernandez, Franco Daniel
author_facet Fernandez, Franco Daniel
Galdeano, Ernestina
Conci, Luis Rogelio
author_role author
author2 Galdeano, Ernestina
Conci, Luis Rogelio
author2_role author
author
dc.subject.none.fl_str_mv Phytoplasmas
Weeds
Phyllody
Witches' Broom
Classification
Filodia
Fitoplasmas
Malezas
Clasificación
Argentina
Phytoplasma Diversity
topic Phytoplasmas
Weeds
Phyllody
Witches' Broom
Classification
Filodia
Fitoplasmas
Malezas
Clasificación
Argentina
Phytoplasma Diversity
dc.description.none.fl_txt_mv Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X) B. subalternans, H. ovatifolium, and C. maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B) and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-( B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X) B. subalternans, H. ovatifolium, and C. maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B) and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-( B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-03
2021-06-25T18:36:15Z
2021-06-25T18:36:15Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9668
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003704
1466-5026
1466-5034 (online)
https://doi.org/10.1099/ijsem.0.003704
url http://hdl.handle.net/20.500.12123/9668
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003704
https://doi.org/10.1099/ijsem.0.003704
identifier_str_mv 1466-5026
1466-5034 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.
info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.
info:eu-repograntAgreement/INTA/PNIND-1108071/AR./Estrategias de manejo de sistemas productivos resilientes.
info:eu-repograntAgreement/INTA/PNIND-1108072/AR./Epidemiología de plagas y enfermedades en cultivos industriales con enfoque al desarrollo de estrategias de manejo integrado.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv International Journal of Systematic and Evolutionary Microbiology 70 (1) : 35-43 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842975498762715136
score 12.993085

Publicaciones similares