Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
- Autores
- Mignaqui, Ana Clara; Ruiz, Vanesa; Perret, Sylvie; St-Laurent, Gilles; Chahal, Parminder Singh; Transfiguracion, Julia; Sammarruco, Romina Ayelén; Gnazzo, Victoria; Durocher, Yves; Wigdorovitz, Andres
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- PLoS ONE 8 (8) : e72800. (August 20, 2013)
- Materia
-
Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4809
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Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsidsMignaqui, Ana ClaraRuiz, VanesaPerret, SylvieSt-Laurent, GillesChahal, Parminder SinghTransfiguracion, JuliaSammarruco, Romina AyelénGnazzo, VictoriaDurocher, YvesWigdorovitz, AndresExpresión GénicaMamíferosVirus Fiebre AftosaRecombinaciónTransfecciónVacunaProteasasGene ExpressionMammalsAphthovirusRecombinationTransfectionVaccinesProteasesFoot and Mouth Disease VirusCapsidsCápsidasFoot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Instituto de VirologíaFil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPLOS2019-04-03T17:36:27Z2019-04-03T17:36:27Z2013-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4809https://journals.plos.org/plosone/article?id=10.1371/journal.pone.00728001932-6203https://doi.org/10.1371/journal.pone.0072800PLoS ONE 8 (8) : e72800. (August 20, 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:37Zoai:localhost:20.500.12123/4809instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:37.623INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
title |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
spellingShingle |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids Mignaqui, Ana Clara Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas |
title_short |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
title_full |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
title_fullStr |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
title_full_unstemmed |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
title_sort |
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids |
dc.creator.none.fl_str_mv |
Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres |
author |
Mignaqui, Ana Clara |
author_facet |
Mignaqui, Ana Clara Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres |
author_role |
author |
author2 |
Ruiz, Vanesa Perret, Sylvie St-Laurent, Gilles Chahal, Parminder Singh Transfiguracion, Julia Sammarruco, Romina Ayelén Gnazzo, Victoria Durocher, Yves Wigdorovitz, Andres |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas |
topic |
Expresión Génica Mamíferos Virus Fiebre Aftosa Recombinación Transfección Vacuna Proteasas Gene Expression Mammals Aphthovirus Recombination Transfection Vaccines Proteases Foot and Mouth Disease Virus Capsids Cápsidas |
dc.description.none.fl_txt_mv |
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Instituto de Virología Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-20 2019-04-03T17:36:27Z 2019-04-03T17:36:27Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4809 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800 1932-6203 https://doi.org/10.1371/journal.pone.0072800 |
url |
http://hdl.handle.net/20.500.12123/4809 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800 https://doi.org/10.1371/journal.pone.0072800 |
identifier_str_mv |
1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosa |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
PLoS ONE 8 (8) : e72800. (August 20, 2013) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.559606 |