Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids

Autores
Mignaqui, Ana Clara; Ruiz, Vanesa; Perret, Sylvie; St-Laurent, Gilles; Chahal, Parminder Singh; Transfiguracion, Julia; Sammarruco, Romina Ayelén; Gnazzo, Victoria; Durocher, Yves; Wigdorovitz, Andres
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
PLoS ONE 8 (8) : e72800. (August 20, 2013)
Materia
Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsidsMignaqui, Ana ClaraRuiz, VanesaPerret, SylvieSt-Laurent, GillesChahal, Parminder SinghTransfiguracion, JuliaSammarruco, Romina AyelénGnazzo, VictoriaDurocher, YvesWigdorovitz, AndresExpresión GénicaMamíferosVirus Fiebre AftosaRecombinaciónTransfecciónVacunaProteasasGene ExpressionMammalsAphthovirusRecombinationTransfectionVaccinesProteasesFoot and Mouth Disease VirusCapsidsCápsidasFoot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Instituto de VirologíaFil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; CanadáFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPLOS2019-04-03T17:36:27Z2019-04-03T17:36:27Z2013-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4809https://journals.plos.org/plosone/article?id=10.1371/journal.pone.00728001932-6203https://doi.org/10.1371/journal.pone.0072800PLoS ONE 8 (8) : e72800. (August 20, 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:37Zoai:localhost:20.500.12123/4809instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:37.623INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
title Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
spellingShingle Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
Mignaqui, Ana Clara
Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
title_short Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
title_full Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
title_fullStr Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
title_full_unstemmed Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
title_sort Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
dc.creator.none.fl_str_mv Mignaqui, Ana Clara
Ruiz, Vanesa
Perret, Sylvie
St-Laurent, Gilles
Chahal, Parminder Singh
Transfiguracion, Julia
Sammarruco, Romina Ayelén
Gnazzo, Victoria
Durocher, Yves
Wigdorovitz, Andres
author Mignaqui, Ana Clara
author_facet Mignaqui, Ana Clara
Ruiz, Vanesa
Perret, Sylvie
St-Laurent, Gilles
Chahal, Parminder Singh
Transfiguracion, Julia
Sammarruco, Romina Ayelén
Gnazzo, Victoria
Durocher, Yves
Wigdorovitz, Andres
author_role author
author2 Ruiz, Vanesa
Perret, Sylvie
St-Laurent, Gilles
Chahal, Parminder Singh
Transfiguracion, Julia
Sammarruco, Romina Ayelén
Gnazzo, Victoria
Durocher, Yves
Wigdorovitz, Andres
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
topic Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
dc.description.none.fl_txt_mv Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadá
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-20
2019-04-03T17:36:27Z
2019-04-03T17:36:27Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4809
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800
1932-6203
https://doi.org/10.1371/journal.pone.0072800
url http://hdl.handle.net/20.500.12123/4809
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800
https://doi.org/10.1371/journal.pone.0072800
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv PLoS ONE 8 (8) : e72800. (August 20, 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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