BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle

Autores
Carignano, Hugo Adrian; Beribe, Maria Jose; Caffaro, Marí­a Eugenia; Amadio, Ariel; Nani, Juan Pablo; Gutierrez, Gerónimo; Alvarez, Irene; Trono, Karina Gabriela; Miretti, Marcos Mateo; Poli, Mario Andres
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile.
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Poli, Mario AndresInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fuente
Animal genetics 48 (4) : 420–430. (August 2017)
Materia
Enfermedades de los Animales
Ganado Bovino
Animal Diseases
Cattle
Bovine Leukaemia Virus
Breeds (Animals)
Crossbreeding
Polymorphism
Genetics
Virus Leucemia Bovina
Razas (Animales)
Cruzamiento
Polimorfismo
Genética
Raza Holstein
Raza Jersey
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1430
Acceder
id INTADig_88c349c71356d91f5347f10017178535
oai_identifier_str oai:localhost:20.500.12123/1430
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattleCarignano, Hugo AdrianBeribe, Maria JoseCaffaro, Marí­a EugeniaAmadio, ArielNani, Juan PabloGutierrez, GerónimoAlvarez, IreneTrono, Karina GabrielaMiretti, Marcos MateoPoli, Mario AndresEnfermedades de los AnimalesGanado BovinoAnimal DiseasesCattleBovine Leukaemia VirusBreeds (Animals)CrossbreedingPolymorphismGeneticsVirus Leucemia BovinaRazas (Animales)CruzamientoPolimorfismoGenéticaRaza HolsteinRaza JerseyBovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile.Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; ArgentinaFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Poli, Mario AndresInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina2017-10-06T14:35:02Z2017-10-06T14:35:02Z2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1430http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t020268-9146 (Print)1365-2052 (Online)https://doi.org/10.1111/age.12566Animal genetics 48 (4) : 420–430. (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:05Zoai:localhost:20.500.12123/1430instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:06.226INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
title BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
spellingShingle BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
Carignano, Hugo Adrian
Enfermedades de los Animales
Ganado Bovino
Animal Diseases
Cattle
Bovine Leukaemia Virus
Breeds (Animals)
Crossbreeding
Polymorphism
Genetics
Virus Leucemia Bovina
Razas (Animales)
Cruzamiento
Polimorfismo
Genética
Raza Holstein
Raza Jersey
title_short BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
title_full BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
title_fullStr BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
title_full_unstemmed BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
title_sort BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
dc.creator.none.fl_str_mv Carignano, Hugo Adrian
Beribe, Maria Jose
Caffaro, Marí­a Eugenia
Amadio, Ariel
Nani, Juan Pablo
Gutierrez, Gerónimo
Alvarez, Irene
Trono, Karina Gabriela
Miretti, Marcos Mateo
Poli, Mario Andres
author Carignano, Hugo Adrian
author_facet Carignano, Hugo Adrian
Beribe, Maria Jose
Caffaro, Marí­a Eugenia
Amadio, Ariel
Nani, Juan Pablo
Gutierrez, Gerónimo
Alvarez, Irene
Trono, Karina Gabriela
Miretti, Marcos Mateo
Poli, Mario Andres
author_role author
author2 Beribe, Maria Jose
Caffaro, Marí­a Eugenia
Amadio, Ariel
Nani, Juan Pablo
Gutierrez, Gerónimo
Alvarez, Irene
Trono, Karina Gabriela
Miretti, Marcos Mateo
Poli, Mario Andres
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Ganado Bovino
Animal Diseases
Cattle
Bovine Leukaemia Virus
Breeds (Animals)
Crossbreeding
Polymorphism
Genetics
Virus Leucemia Bovina
Razas (Animales)
Cruzamiento
Polimorfismo
Genética
Raza Holstein
Raza Jersey
topic Enfermedades de los Animales
Ganado Bovino
Animal Diseases
Cattle
Bovine Leukaemia Virus
Breeds (Animals)
Crossbreeding
Polymorphism
Genetics
Virus Leucemia Bovina
Razas (Animales)
Cruzamiento
Polimorfismo
Genética
Raza Holstein
Raza Jersey
dc.description.none.fl_txt_mv Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile.
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Poli, Mario AndresInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
description Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-06T14:35:02Z
2017-10-06T14:35:02Z
2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1430
http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02
0268-9146 (Print)
1365-2052 (Online)
https://doi.org/10.1111/age.12566
url http://hdl.handle.net/20.500.12123/1430
http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02
https://doi.org/10.1111/age.12566
identifier_str_mv 0268-9146 (Print)
1365-2052 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Animal genetics 48 (4) : 420–430. (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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