3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets

Autores
Garcia, Florencia; Muñoz, Camila; Martinez Ferrer, Jorge; Urrutia, Natalie L.; Martínez, Emilio D.; Saldivia, Marcelo; Immig, Irmgard; Kindermann, Maik; Walker, Nicola; Ungerfeld, Emilio M.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources.
EEA Manfredi
Fil: Garcia, Florencia. Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias; Argentina
Fil: Muñoz, Camila. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile
Fil: Martinez Ferrer, Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Urrutia, Natalie L. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile
Fil: Martínez, Emilio D. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile
Fil: Saldivia, Marcelo. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile
Fil: Immig, Irmgard. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil: Kindermann, Maik. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil: Walker, Nicola. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil. Ungerfeld, Emilio M. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Carillanca; Chile
Fuente
Heliyon 8 : e09738. (2022)
Materia
Producción Lechera
Dietas Restrictas en Proteínas
Metano
Efecto Invernadero
Milk Production
Protein Restricted Diets
Methane
Greenhouse Effect
Dairy Cows
Vacas Lecheras
3-nitrooxipropanol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/17680
Acceder
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oai_identifier_str oai:localhost:20.500.12123/17680
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing dietsGarcia, FlorenciaMuñoz, CamilaMartinez Ferrer, JorgeUrrutia, Natalie L.Martínez, Emilio D.Saldivia, MarceloImmig, IrmgardKindermann, MaikWalker, NicolaUngerfeld, Emilio M.Producción LecheraDietas Restrictas en ProteínasMetanoEfecto InvernaderoMilk ProductionProtein Restricted DietsMethaneGreenhouse EffectDairy CowsVacas Lecheras3-nitrooxipropanolMethane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources.EEA ManfrediFil: Garcia, Florencia. Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias; ArgentinaFil: Muñoz, Camila. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; ChileFil: Martinez Ferrer, Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Urrutia, Natalie L. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; ChileFil: Martínez, Emilio D. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; ChileFil: Saldivia, Marcelo. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; ChileFil: Immig, Irmgard. DSM Nutritional Products. Animal Nutrition and Health; SuizaFil: Kindermann, Maik. DSM Nutritional Products. Animal Nutrition and Health; SuizaFil: Walker, Nicola. DSM Nutritional Products. Animal Nutrition and Health; SuizaFil. Ungerfeld, Emilio M. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Carillanca; ChileElsevier2024-05-09T10:31:21Z2024-05-09T10:31:21Z2022-06-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17680https://www.sciencedirect.com/science/article/pii/S240584402201026Xhttps://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X2405-8440https://doi.org/10.1016/j.heliyon.2022.e09738Heliyon 8 : e09738. (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:36Zoai:localhost:20.500.12123/17680instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:37.246INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
title 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
spellingShingle 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
Garcia, Florencia
Producción Lechera
Dietas Restrictas en Proteínas
Metano
Efecto Invernadero
Milk Production
Protein Restricted Diets
Methane
Greenhouse Effect
Dairy Cows
Vacas Lecheras
3-nitrooxipropanol
title_short 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
title_full 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
title_fullStr 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
title_full_unstemmed 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
title_sort 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
dc.creator.none.fl_str_mv Garcia, Florencia
Muñoz, Camila
Martinez Ferrer, Jorge
Urrutia, Natalie L.
Martínez, Emilio D.
Saldivia, Marcelo
Immig, Irmgard
Kindermann, Maik
Walker, Nicola
Ungerfeld, Emilio M.
author Garcia, Florencia
author_facet Garcia, Florencia
Muñoz, Camila
Martinez Ferrer, Jorge
Urrutia, Natalie L.
Martínez, Emilio D.
Saldivia, Marcelo
Immig, Irmgard
Kindermann, Maik
Walker, Nicola
Ungerfeld, Emilio M.
author_role author
author2 Muñoz, Camila
Martinez Ferrer, Jorge
Urrutia, Natalie L.
Martínez, Emilio D.
Saldivia, Marcelo
Immig, Irmgard
Kindermann, Maik
Walker, Nicola
Ungerfeld, Emilio M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Producción Lechera
Dietas Restrictas en Proteínas
Metano
Efecto Invernadero
Milk Production
Protein Restricted Diets
Methane
Greenhouse Effect
Dairy Cows
Vacas Lecheras
3-nitrooxipropanol
topic Producción Lechera
Dietas Restrictas en Proteínas
Metano
Efecto Invernadero
Milk Production
Protein Restricted Diets
Methane
Greenhouse Effect
Dairy Cows
Vacas Lecheras
3-nitrooxipropanol
dc.description.none.fl_txt_mv Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources.
EEA Manfredi
Fil: Garcia, Florencia. Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias; Argentina
Fil: Muñoz, Camila. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile
Fil: Martinez Ferrer, Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Urrutia, Natalie L. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile
Fil: Martínez, Emilio D. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile
Fil: Saldivia, Marcelo. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile
Fil: Immig, Irmgard. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil: Kindermann, Maik. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil: Walker, Nicola. DSM Nutritional Products. Animal Nutrition and Health; Suiza
Fil. Ungerfeld, Emilio M. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Carillanca; Chile
description Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-16
2024-05-09T10:31:21Z
2024-05-09T10:31:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/17680
https://www.sciencedirect.com/science/article/pii/S240584402201026X
https://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X
2405-8440
https://doi.org/10.1016/j.heliyon.2022.e09738
url http://hdl.handle.net/20.500.12123/17680
https://www.sciencedirect.com/science/article/pii/S240584402201026X
https://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X
https://doi.org/10.1016/j.heliyon.2022.e09738
identifier_str_mv 2405-8440
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Heliyon 8 : e09738. (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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