Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata

Autores
Delprat, María Alejandra; Stolar, Cristian Eric; Manso, Fanny Celia; Cladera Jorge Luis
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT).
Instituto de Genética
Fil: Delprat, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Stolar, Cristian Eric. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Manso, Fanny Celia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Genetica 116 : 85–95 (2002)
Materia
Ceratitis capitata
Plagas de Plantas
Estabilidad Genética
Recombinación
Pests of Plants
Genetic Stability
Recombination
Mosca del Mediterráneo
Recombinación Genética
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7529
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7529
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spelling Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis CapitataDelprat, María AlejandraStolar, Cristian EricManso, Fanny CeliaCladera Jorge LuisCeratitis capitataPlagas de PlantasEstabilidad GenéticaRecombinaciónPests of PlantsGenetic StabilityRecombinationMosca del MediterráneoRecombinación GenéticaThis report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT).Instituto de GenéticaFil: Delprat, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Stolar, Cristian Eric. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Manso, Fanny Celia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2020-07-07T17:44:51Z2020-07-07T17:44:51Z2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/75290016-67071573-6857https://doi.org/10.1023/A:1020963709795Genetica 116 : 85–95 (2002)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:58Zoai:localhost:20.500.12123/7529instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:59.188INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
title Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
spellingShingle Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
Delprat, María Alejandra
Ceratitis capitata
Plagas de Plantas
Estabilidad Genética
Recombinación
Pests of Plants
Genetic Stability
Recombination
Mosca del Mediterráneo
Recombinación Genética
title_short Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
title_full Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
title_fullStr Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
title_full_unstemmed Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
title_sort Genetic Stability of Sexing Strains Based on the Locus swof Ceratitis Capitata
dc.creator.none.fl_str_mv Delprat, María Alejandra
Stolar, Cristian Eric
Manso, Fanny Celia
Cladera Jorge Luis
author Delprat, María Alejandra
author_facet Delprat, María Alejandra
Stolar, Cristian Eric
Manso, Fanny Celia
Cladera Jorge Luis
author_role author
author2 Stolar, Cristian Eric
Manso, Fanny Celia
Cladera Jorge Luis
author2_role author
author
author
dc.subject.none.fl_str_mv Ceratitis capitata
Plagas de Plantas
Estabilidad Genética
Recombinación
Pests of Plants
Genetic Stability
Recombination
Mosca del Mediterráneo
Recombinación Genética
topic Ceratitis capitata
Plagas de Plantas
Estabilidad Genética
Recombinación
Pests of Plants
Genetic Stability
Recombination
Mosca del Mediterráneo
Recombinación Genética
dc.description.none.fl_txt_mv This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT).
Instituto de Genética
Fil: Delprat, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Stolar, Cristian Eric. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Manso, Fanny Celia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the swmutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping swwith deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of swis region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast191 was shown to be the most promising as no recombination between swand the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 × 10−6). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT).
publishDate 2002
dc.date.none.fl_str_mv 2002-09
2020-07-07T17:44:51Z
2020-07-07T17:44:51Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7529
0016-6707
1573-6857
https://doi.org/10.1023/A:1020963709795
url http://hdl.handle.net/20.500.12123/7529
https://doi.org/10.1023/A:1020963709795
identifier_str_mv 0016-6707
1573-6857
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Genetica 116 : 85–95 (2002)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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