Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
- Autores
- Erreguerena, Ignacio Antonio; Havis, Neil David; Heick, Thies Marten; Gorniak, Kalina; Quiroz, Facundo Jose; Carmona, Marcelo Anibal
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.
EEA Manfredi
Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Heick, Thies Marten. Aarhus University. Department of Agroecology; Dinamarca
Fil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina - Fuente
- Journal of Plant Diseases and Protection 129 : 1-11 (2022)
- Materia
-
Cebada
Boletus edulis
Mutación
Fungicidas
Barley
Mutation
Fungicides
Ramularia
Hordeum vulgare
Argentina - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/12974
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Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in ArgentinaErreguerena, Ignacio AntonioHavis, Neil DavidHeick, Thies MartenGorniak, KalinaQuiroz, Facundo JoseCarmona, Marcelo AnibalCebadaBoletus edulisMutaciónFungicidasBarleyMutationFungicidesRamulariaHordeum vulgareArgentinaRamularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.EEA ManfrediFil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino UnidoFil: Heick, Thies Marten. Aarhus University. Department of Agroecology; DinamarcaFil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino UnidoFil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; ArgentinaSpringer2022-09-27T10:16:40Z2022-09-27T10:16:40Z2022-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12974https://link.springer.com/article/10.1007/s41348-022-00645-01861-3837https://doi.org/10.1007/s41348-022-00645-0Journal of Plant Diseases and Protection 129 : 1-11 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.info:eu-repo/semantics/restrictedAccess2025-09-04T09:49:34Zoai:localhost:20.500.12123/12974instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:34.626INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
title |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
spellingShingle |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina Erreguerena, Ignacio Antonio Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina |
title_short |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
title_full |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
title_fullStr |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
title_full_unstemmed |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
title_sort |
Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
dc.creator.none.fl_str_mv |
Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal |
author |
Erreguerena, Ignacio Antonio |
author_facet |
Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal |
author_role |
author |
author2 |
Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina |
topic |
Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina |
dc.description.none.fl_txt_mv |
Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies. EEA Manfredi Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido Fil: Heick, Thies Marten. Aarhus University. Department of Agroecology; Dinamarca Fil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido Fil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina |
description |
Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-27T10:16:40Z 2022-09-27T10:16:40Z 2022-06-23 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/12974 https://link.springer.com/article/10.1007/s41348-022-00645-0 1861-3837 https://doi.org/10.1007/s41348-022-00645-0 |
url |
http://hdl.handle.net/20.500.12123/12974 https://link.springer.com/article/10.1007/s41348-022-00645-0 https://doi.org/10.1007/s41348-022-00645-0 |
identifier_str_mv |
1861-3837 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas. info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Journal of Plant Diseases and Protection 129 : 1-11 (2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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