Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina

Autores
Erreguerena, Ignacio Antonio; Havis, Neil David; Heick, Thies Marten; Gorniak, Kalina; Quiroz, Facundo Jose; Carmona, Marcelo Anibal
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.
EEA Manfredi
Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Heick, Thies Marten. Aarhus University. Department of Agroecology; Dinamarca
Fil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina
Fuente
Journal of Plant Diseases and Protection 129 : 1-11 (2022)
Materia
Cebada
Boletus edulis
Mutación
Fungicidas
Barley
Mutation
Fungicides
Ramularia
Hordeum vulgare
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in ArgentinaErreguerena, Ignacio AntonioHavis, Neil DavidHeick, Thies MartenGorniak, KalinaQuiroz, Facundo JoseCarmona, Marcelo AnibalCebadaBoletus edulisMutaciónFungicidasBarleyMutationFungicidesRamulariaHordeum vulgareArgentinaRamularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.EEA ManfrediFil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino UnidoFil: Heick, Thies Marten. Aarhus University. Department of Agroecology; DinamarcaFil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino UnidoFil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; ArgentinaSpringer2022-09-27T10:16:40Z2022-09-27T10:16:40Z2022-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12974https://link.springer.com/article/10.1007/s41348-022-00645-01861-3837https://doi.org/10.1007/s41348-022-00645-0Journal of Plant Diseases and Protection 129 : 1-11 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:43Zoai:localhost:20.500.12123/12974instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:44.095INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
title Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
spellingShingle Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
Erreguerena, Ignacio Antonio
Cebada
Boletus edulis
Mutación
Fungicidas
Barley
Mutation
Fungicides
Ramularia
Hordeum vulgare
Argentina
title_short Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
title_full Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
title_fullStr Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
title_full_unstemmed Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
title_sort Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
dc.creator.none.fl_str_mv Erreguerena, Ignacio Antonio
Havis, Neil David
Heick, Thies Marten
Gorniak, Kalina
Quiroz, Facundo Jose
Carmona, Marcelo Anibal
author Erreguerena, Ignacio Antonio
author_facet Erreguerena, Ignacio Antonio
Havis, Neil David
Heick, Thies Marten
Gorniak, Kalina
Quiroz, Facundo Jose
Carmona, Marcelo Anibal
author_role author
author2 Havis, Neil David
Heick, Thies Marten
Gorniak, Kalina
Quiroz, Facundo Jose
Carmona, Marcelo Anibal
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cebada
Boletus edulis
Mutación
Fungicidas
Barley
Mutation
Fungicides
Ramularia
Hordeum vulgare
Argentina
topic Cebada
Boletus edulis
Mutación
Fungicidas
Barley
Mutation
Fungicides
Ramularia
Hordeum vulgare
Argentina
dc.description.none.fl_txt_mv Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.
EEA Manfredi
Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Heick, Thies Marten. Aarhus University. Department of Agroecology; Dinamarca
Fil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido
Fil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina
description Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-27T10:16:40Z
2022-09-27T10:16:40Z
2022-06-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12974
https://link.springer.com/article/10.1007/s41348-022-00645-0
1861-3837
https://doi.org/10.1007/s41348-022-00645-0
url http://hdl.handle.net/20.500.12123/12974
https://link.springer.com/article/10.1007/s41348-022-00645-0
https://doi.org/10.1007/s41348-022-00645-0
identifier_str_mv 1861-3837
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.
info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Plant Diseases and Protection 129 : 1-11 (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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