Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull

Autores
García, Juan Agustín; Gioffre, Andrea; Acuña, Juaquín; Mendez, Maria Alejandra; Morsella, Claudia Graciela; Aller Atucha, Juan Florencio; Paolicchi, Fernando
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle.
EEA Balcarce
Fil: García, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gioffré, Andrea K. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Méndez, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fuente
Veterinary science communications (september 2021)
Materia
Bovinae
Rumiante
Enfermedad de Transmición Sexual
Campilobacteriosis
Glándulas Vesiculares
Ruminants
Sexually Transmitted Diseases
Campylobacteriosis
Vesicular Glands
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10233
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10233
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bullGarcía, Juan AgustínGioffre, AndreaAcuña, JuaquínMendez, Maria AlejandraMorsella, Claudia GracielaAller Atucha, Juan FlorencioPaolicchi, FernandoBovinaeRumianteEnfermedad de Transmición SexualCampilobacteriosisGlándulas VesicularesRuminantsSexually Transmitted DiseasesCampylobacteriosisVesicular GlandsCampylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle.EEA BalcarceFil: García, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Gioffré, Andrea K. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Acuña, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Méndez, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Springer Verlag2021-09-13T13:06:06Z2021-09-13T13:06:06Z2021-09-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10233http://link.springer.com/10.1007/s11259-021-09823-11573-7446 (on line)0165-7380 (print)https://doi.org/10.1007/s11259-021-09823-1Veterinary science communications (september 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicainfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”info:eu-repo/semantics/restrictedAccess2025-09-04T09:49:05Zoai:localhost:20.500.12123/10233instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:06.122INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
title Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
spellingShingle Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
García, Juan Agustín
Bovinae
Rumiante
Enfermedad de Transmición Sexual
Campilobacteriosis
Glándulas Vesiculares
Ruminants
Sexually Transmitted Diseases
Campylobacteriosis
Vesicular Glands
title_short Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
title_full Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
title_fullStr Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
title_full_unstemmed Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
title_sort Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
dc.creator.none.fl_str_mv García, Juan Agustín
Gioffre, Andrea
Acuña, Juaquín
Mendez, Maria Alejandra
Morsella, Claudia Graciela
Aller Atucha, Juan Florencio
Paolicchi, Fernando
author García, Juan Agustín
author_facet García, Juan Agustín
Gioffre, Andrea
Acuña, Juaquín
Mendez, Maria Alejandra
Morsella, Claudia Graciela
Aller Atucha, Juan Florencio
Paolicchi, Fernando
author_role author
author2 Gioffre, Andrea
Acuña, Juaquín
Mendez, Maria Alejandra
Morsella, Claudia Graciela
Aller Atucha, Juan Florencio
Paolicchi, Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovinae
Rumiante
Enfermedad de Transmición Sexual
Campilobacteriosis
Glándulas Vesiculares
Ruminants
Sexually Transmitted Diseases
Campylobacteriosis
Vesicular Glands
topic Bovinae
Rumiante
Enfermedad de Transmición Sexual
Campilobacteriosis
Glándulas Vesiculares
Ruminants
Sexually Transmitted Diseases
Campylobacteriosis
Vesicular Glands
dc.description.none.fl_txt_mv Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle.
EEA Balcarce
Fil: García, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gioffré, Andrea K. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Méndez, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
description Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-13T13:06:06Z
2021-09-13T13:06:06Z
2021-09-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10233
http://link.springer.com/10.1007/s11259-021-09823-1
1573-7446 (on line)
0165-7380 (print)
https://doi.org/10.1007/s11259-021-09823-1
url http://hdl.handle.net/20.500.12123/10233
http://link.springer.com/10.1007/s11259-021-09823-1
https://doi.org/10.1007/s11259-021-09823-1
identifier_str_mv 1573-7446 (on line)
0165-7380 (print)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública
info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Veterinary science communications (september 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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