Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)

Autores
Bergottini, Veronica M.; Hervé, Vincent; Sosa, Domingo Alberto; Otegui, Mónica B.; Zapata, Pedro Darío; Junier, Pilar
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop.
Fil: Bergottini, Veronica M. University of Neuchâtel. Laboratory of Microbiology; Suiza
Fil: Hervé, Vincent. University of Neuchâtel. Laboratory of Microbiology; Suiza. University of Lausanne. Institute of Earth Surface Dynamics. Laboratory of Biogeosciences; Suiza
Fil: Sosa, Domingo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Otegui, Mónica B. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina
Fil: Junier, Pilar. University of Neuchâtel. Laboratory of Microbiology; Suiza
Fuente
Applied soil ecology 109 : 23–31. (2017)
Materia
Ilex Paraguariensis
Mate
Microorganismos
Raíces
Burkholderiaceae
Mycorrhizae
Rhizobacteria
Rizobacterias
Roots
Microorganisms
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2791

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spelling Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)Bergottini, Veronica M.Hervé, VincentSosa, Domingo AlbertoOtegui, Mónica B.Zapata, Pedro DaríoJunier, PilarIlex ParaguariensisMateMicroorganismosRaícesBurkholderiaceaeMycorrhizaeRhizobacteriaRizobacteriasRootsMicroorganismsIlex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop.Fil: Bergottini, Veronica M. University of Neuchâtel. Laboratory of Microbiology; SuizaFil: Hervé, Vincent. University of Neuchâtel. Laboratory of Microbiology; Suiza. University of Lausanne. Institute of Earth Surface Dynamics. Laboratory of Biogeosciences; SuizaFil: Sosa, Domingo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Otegui, Mónica B. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; ArgentinaFil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; ArgentinaFil: Junier, Pilar. University of Neuchâtel. Laboratory of Microbiology; Suiza2018-07-13T15:55:17Z2018-07-13T15:55:17Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27910929-1393http://dx.doi.org/10.1016/j.apsoil.2016.09.013Applied soil ecology 109 : 23–31. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:21Zoai:localhost:20.500.12123/2791instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:22.288INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
title Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
spellingShingle Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
Bergottini, Veronica M.
Ilex Paraguariensis
Mate
Microorganismos
Raíces
Burkholderiaceae
Mycorrhizae
Rhizobacteria
Rizobacterias
Roots
Microorganisms
title_short Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
title_full Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
title_fullStr Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
title_full_unstemmed Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
title_sort Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
dc.creator.none.fl_str_mv Bergottini, Veronica M.
Hervé, Vincent
Sosa, Domingo Alberto
Otegui, Mónica B.
Zapata, Pedro Darío
Junier, Pilar
author Bergottini, Veronica M.
author_facet Bergottini, Veronica M.
Hervé, Vincent
Sosa, Domingo Alberto
Otegui, Mónica B.
Zapata, Pedro Darío
Junier, Pilar
author_role author
author2 Hervé, Vincent
Sosa, Domingo Alberto
Otegui, Mónica B.
Zapata, Pedro Darío
Junier, Pilar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ilex Paraguariensis
Mate
Microorganismos
Raíces
Burkholderiaceae
Mycorrhizae
Rhizobacteria
Rizobacterias
Roots
Microorganisms
topic Ilex Paraguariensis
Mate
Microorganismos
Raíces
Burkholderiaceae
Mycorrhizae
Rhizobacteria
Rizobacterias
Roots
Microorganisms
dc.description.none.fl_txt_mv Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop.
Fil: Bergottini, Veronica M. University of Neuchâtel. Laboratory of Microbiology; Suiza
Fil: Hervé, Vincent. University of Neuchâtel. Laboratory of Microbiology; Suiza. University of Lausanne. Institute of Earth Surface Dynamics. Laboratory of Biogeosciences; Suiza
Fil: Sosa, Domingo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Otegui, Mónica B. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina
Fil: Junier, Pilar. University of Neuchâtel. Laboratory of Microbiology; Suiza
description Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-07-13T15:55:17Z
2018-07-13T15:55:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2791
0929-1393
http://dx.doi.org/10.1016/j.apsoil.2016.09.013
url http://hdl.handle.net/20.500.12123/2791
http://dx.doi.org/10.1016/j.apsoil.2016.09.013
identifier_str_mv 0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Applied soil ecology 109 : 23–31. (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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