Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia

Autores
Soleño, Jimena; Anguiano, Olga Liliana; Pechen de D’Angelo, Ana; Cichon, Liliana; Fernandez, Dario Eduardo; Montagna, Cristina Mónica
Año de publicación
2008
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry
EEA Alto Valle
Fil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; Argentina
Fuente
Pest Management Science 64 (9): 964–970 (September 2008)
Materia
Cydia Pomonella
Resistencia a las plagas
Control de plagas
Insecticides
Insecticidas
Esterasas
Rio Negro (Argentina)
Neuquén
Pest resistance
Pest control
Esterases
Codling Moth
Polilla de la Manzana
Carpocapsa
Azinphos-Methyl
Azinfos-Metil
Glutathione S-Transferases
Glutatión S-Transferasas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21113
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21113
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian PatagoniaSoleño, JimenaAnguiano, Olga LilianaPechen de D’Angelo, AnaCichon, LilianaFernandez, Dario EduardoMontagna, Cristina MónicaCydia PomonellaResistencia a las plagasControl de plagasInsecticidesInsecticidasEsterasasRio Negro (Argentina)NeuquénPest resistancePest controlEsterasesCodling MothPolilla de la ManzanaCarpocapsaAzinphos-MethylAzinfos-MetilGlutathione S-TransferasesGlutatión S-TransferasasBACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical IndustryEEA Alto ValleFil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; ArgentinaWiley2025-01-31T14:28:48Z2025-01-31T14:28:48Z2008-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21113https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.15821526-49981526-498Xhttps://doi.org/10.1002/ps.1582Pest Management Science 64 (9): 964–970 (September 2008)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:08Zoai:localhost:20.500.12123/21113instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:08.751INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
title Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
spellingShingle Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
Soleño, Jimena
Cydia Pomonella
Resistencia a las plagas
Control de plagas
Insecticides
Insecticidas
Esterasas
Rio Negro (Argentina)
Neuquén
Pest resistance
Pest control
Esterases
Codling Moth
Polilla de la Manzana
Carpocapsa
Azinphos-Methyl
Azinfos-Metil
Glutathione S-Transferases
Glutatión S-Transferasas
title_short Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
title_full Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
title_fullStr Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
title_full_unstemmed Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
title_sort Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
dc.creator.none.fl_str_mv Soleño, Jimena
Anguiano, Olga Liliana
Pechen de D’Angelo, Ana
Cichon, Liliana
Fernandez, Dario Eduardo
Montagna, Cristina Mónica
author Soleño, Jimena
author_facet Soleño, Jimena
Anguiano, Olga Liliana
Pechen de D’Angelo, Ana
Cichon, Liliana
Fernandez, Dario Eduardo
Montagna, Cristina Mónica
author_role author
author2 Anguiano, Olga Liliana
Pechen de D’Angelo, Ana
Cichon, Liliana
Fernandez, Dario Eduardo
Montagna, Cristina Mónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cydia Pomonella
Resistencia a las plagas
Control de plagas
Insecticides
Insecticidas
Esterasas
Rio Negro (Argentina)
Neuquén
Pest resistance
Pest control
Esterases
Codling Moth
Polilla de la Manzana
Carpocapsa
Azinphos-Methyl
Azinfos-Metil
Glutathione S-Transferases
Glutatión S-Transferasas
topic Cydia Pomonella
Resistencia a las plagas
Control de plagas
Insecticides
Insecticidas
Esterasas
Rio Negro (Argentina)
Neuquén
Pest resistance
Pest control
Esterases
Codling Moth
Polilla de la Manzana
Carpocapsa
Azinphos-Methyl
Azinfos-Metil
Glutathione S-Transferases
Glutatión S-Transferasas
dc.description.none.fl_txt_mv BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry
EEA Alto Valle
Fil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; Argentina
description BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry
publishDate 2008
dc.date.none.fl_str_mv 2008-03-31
2025-01-31T14:28:48Z
2025-01-31T14:28:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21113
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582
1526-4998
1526-498X
https://doi.org/10.1002/ps.1582
url http://hdl.handle.net/20.500.12123/21113
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582
https://doi.org/10.1002/ps.1582
identifier_str_mv 1526-4998
1526-498X
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science 64 (9): 964–970 (September 2008)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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