Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
- Autores
- Soleño, Jimena; Anguiano, Olga Liliana; Pechen de D’Angelo, Ana; Cichon, Liliana; Fernandez, Dario Eduardo; Montagna, Cristina Mónica
- Año de publicación
- 2008
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry
EEA Alto Valle
Fil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina
Fil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; Argentina - Fuente
- Pest Management Science 64 (9): 964–970 (September 2008)
- Materia
-
Cydia Pomonella
Resistencia a las plagas
Control de plagas
Insecticides
Insecticidas
Esterasas
Rio Negro (Argentina)
Neuquén
Pest resistance
Pest control
Esterases
Codling Moth
Polilla de la Manzana
Carpocapsa
Azinphos-Methyl
Azinfos-Metil
Glutathione S-Transferases
Glutatión S-Transferasas - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21113
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Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian PatagoniaSoleño, JimenaAnguiano, Olga LilianaPechen de D’Angelo, AnaCichon, LilianaFernandez, Dario EduardoMontagna, Cristina MónicaCydia PomonellaResistencia a las plagasControl de plagasInsecticidesInsecticidasEsterasasRio Negro (Argentina)NeuquénPest resistancePest controlEsterasesCodling MothPolilla de la ManzanaCarpocapsaAzinphos-MethylAzinfos-MetilGlutathione S-TransferasesGlutatión S-TransferasasBACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical IndustryEEA Alto ValleFil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; ArgentinaFil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; ArgentinaWiley2025-01-31T14:28:48Z2025-01-31T14:28:48Z2008-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21113https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.15821526-49981526-498Xhttps://doi.org/10.1002/ps.1582Pest Management Science 64 (9): 964–970 (September 2008)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:07Zoai:localhost:20.500.12123/21113instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:07.485INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
title |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
spellingShingle |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia Soleño, Jimena Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas |
title_short |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
title_full |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
title_fullStr |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
title_full_unstemmed |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
title_sort |
Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
dc.creator.none.fl_str_mv |
Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica |
author |
Soleño, Jimena |
author_facet |
Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica |
author_role |
author |
author2 |
Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas |
topic |
Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas |
dc.description.none.fl_txt_mv |
BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry EEA Alto Valle Fil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; Argentina |
description |
BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-31 2025-01-31T14:28:48Z 2025-01-31T14:28:48Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/21113 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582 1526-4998 1526-498X https://doi.org/10.1002/ps.1582 |
url |
http://hdl.handle.net/20.500.12123/21113 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582 https://doi.org/10.1002/ps.1582 |
identifier_str_mv |
1526-4998 1526-498X |
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spa |
language |
spa |
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info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Pest Management Science 64 (9): 964–970 (September 2008) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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