Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue

Autores
Martínez Cuesta, Lucía; Nieto Farías, María Victoria; Romeo, Florencia; Verna, Andrea Elizabeth; Pérez, Sandra
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage.
EEA Balcarce
Fil: Martínez Cuesta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
Fil: Nieto Farías, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 90–91: 101906 (November–December 2022)
Materia
Apoptosis
Bovine Herpesvirus
Neuropathy
Cattle
Herpes Virus Bovino
Neuropatía
Ganado Bovino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13612
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13612
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network_name_str INTA Digital (INTA)
spelling Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissueMartínez Cuesta, LucíaNieto Farías, María VictoriaRomeo, FlorenciaVerna, Andrea ElizabethPérez, SandraApoptosisBovine HerpesvirusNeuropathyCattleHerpes Virus BovinoNeuropatíaGanado BovinoBovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage.EEA BalcarceFil: Martínez Cuesta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.Fil: Nieto Farías, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.Elsevier2022-12-14T15:39:45Z2022-12-14T15:39:45Z2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13612https://www.sciencedirect.com/science/article/pii/S01479571220016310147-9571https://doi.org/10.1016/j.cimid.2022.101906Comparative Immunology, Microbiology and Infectious Diseases 90–91: 101906 (November–December 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:40Zoai:localhost:20.500.12123/13612instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:40.662INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
title Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
spellingShingle Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
Martínez Cuesta, Lucía
Apoptosis
Bovine Herpesvirus
Neuropathy
Cattle
Herpes Virus Bovino
Neuropatía
Ganado Bovino
title_short Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
title_full Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
title_fullStr Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
title_full_unstemmed Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
title_sort Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
dc.creator.none.fl_str_mv Martínez Cuesta, Lucía
Nieto Farías, María Victoria
Romeo, Florencia
Verna, Andrea Elizabeth
Pérez, Sandra
author Martínez Cuesta, Lucía
author_facet Martínez Cuesta, Lucía
Nieto Farías, María Victoria
Romeo, Florencia
Verna, Andrea Elizabeth
Pérez, Sandra
author_role author
author2 Nieto Farías, María Victoria
Romeo, Florencia
Verna, Andrea Elizabeth
Pérez, Sandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Bovine Herpesvirus
Neuropathy
Cattle
Herpes Virus Bovino
Neuropatía
Ganado Bovino
topic Apoptosis
Bovine Herpesvirus
Neuropathy
Cattle
Herpes Virus Bovino
Neuropatía
Ganado Bovino
dc.description.none.fl_txt_mv Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage.
EEA Balcarce
Fil: Martínez Cuesta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
Fil: Nieto Farías, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.
description Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-14T15:39:45Z
2022-12-14T15:39:45Z
2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13612
https://www.sciencedirect.com/science/article/pii/S0147957122001631
0147-9571
https://doi.org/10.1016/j.cimid.2022.101906
url http://hdl.handle.net/20.500.12123/13612
https://www.sciencedirect.com/science/article/pii/S0147957122001631
https://doi.org/10.1016/j.cimid.2022.101906
identifier_str_mv 0147-9571
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 90–91: 101906 (November–December 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341406629167104
score 12.623145

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