Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables

Autores
Costa, Magdalena; Sucari, Adriana; Epszteyn, Sergio; Oteiza, Juan Martín; Gentiluomo, Jimena; Melamed, Celia; Figueroa, Yamila; Mingorance, Santiago Emmanuel; Grisaro, Agustina; Spioussas, Silvia; Buffoni Almeida, Mariana; Caruso, Mailen; Pontoni, Andrés; Signorini, Marcelo; Leotta, Gerardo Anibal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory.
EEA Rafaela
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Epszteyn, Sergio. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); Argentina
Fil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Melamed, Celia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Mingorance, Santiago Emmanuel. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Grisaro, Agustina. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Spioussas, Silvia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Buffoni Almeida, Mariana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Caruso, Mailen. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Pontoni, Andrés. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fuente
Food Microbiology 84 : 103273 (December 2019)
Materia
Escherichia coli
Toxinas
Carne
Hortalizas
Identificación
Control de Enfermedades
Toxins
Meat
Vegetables
Identification
Disease Control
Shiga toxin
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5684
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5684
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spelling Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetablesCosta, MagdalenaSucari, AdrianaEpszteyn, SergioOteiza, Juan MartínGentiluomo, JimenaMelamed, CeliaFigueroa, YamilaMingorance, Santiago EmmanuelGrisaro, AgustinaSpioussas, SilviaBuffoni Almeida, MarianaCaruso, MailenPontoni, AndrésSignorini, MarceloLeotta, Gerardo AnibalEscherichia coliToxinasCarneHortalizasIdentificaciónControl de EnfermedadesToxinsMeatVegetablesIdentificationDisease ControlShiga toxinShiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory.EEA RafaelaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Epszteyn, Sergio. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); ArgentinaFil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Melamed, Celia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Mingorance, Santiago Emmanuel. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Grisaro, Agustina. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Spioussas, Silvia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Buffoni Almeida, Mariana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Caruso, Mailen. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Pontoni, Andrés. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaElsevier2019-08-26T11:55:22Z2019-08-26T11:55:22Z2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0740002019304332http://hdl.handle.net/20.500.12123/56840740-00201095-9998https://doi.org/10.1016/j.fm.2019.103273Food Microbiology 84 : 103273 (December 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:08Zoai:localhost:20.500.12123/5684instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:09.142INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
title Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
spellingShingle Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
Costa, Magdalena
Escherichia coli
Toxinas
Carne
Hortalizas
Identificación
Control de Enfermedades
Toxins
Meat
Vegetables
Identification
Disease Control
Shiga toxin
title_short Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
title_full Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
title_fullStr Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
title_full_unstemmed Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
title_sort Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
dc.creator.none.fl_str_mv Costa, Magdalena
Sucari, Adriana
Epszteyn, Sergio
Oteiza, Juan Martín
Gentiluomo, Jimena
Melamed, Celia
Figueroa, Yamila
Mingorance, Santiago Emmanuel
Grisaro, Agustina
Spioussas, Silvia
Buffoni Almeida, Mariana
Caruso, Mailen
Pontoni, Andrés
Signorini, Marcelo
Leotta, Gerardo Anibal
author Costa, Magdalena
author_facet Costa, Magdalena
Sucari, Adriana
Epszteyn, Sergio
Oteiza, Juan Martín
Gentiluomo, Jimena
Melamed, Celia
Figueroa, Yamila
Mingorance, Santiago Emmanuel
Grisaro, Agustina
Spioussas, Silvia
Buffoni Almeida, Mariana
Caruso, Mailen
Pontoni, Andrés
Signorini, Marcelo
Leotta, Gerardo Anibal
author_role author
author2 Sucari, Adriana
Epszteyn, Sergio
Oteiza, Juan Martín
Gentiluomo, Jimena
Melamed, Celia
Figueroa, Yamila
Mingorance, Santiago Emmanuel
Grisaro, Agustina
Spioussas, Silvia
Buffoni Almeida, Mariana
Caruso, Mailen
Pontoni, Andrés
Signorini, Marcelo
Leotta, Gerardo Anibal
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Toxinas
Carne
Hortalizas
Identificación
Control de Enfermedades
Toxins
Meat
Vegetables
Identification
Disease Control
Shiga toxin
topic Escherichia coli
Toxinas
Carne
Hortalizas
Identificación
Control de Enfermedades
Toxins
Meat
Vegetables
Identification
Disease Control
Shiga toxin
dc.description.none.fl_txt_mv Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory.
EEA Rafaela
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Epszteyn, Sergio. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); Argentina
Fil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Melamed, Celia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Mingorance, Santiago Emmanuel. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Grisaro, Agustina. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Spioussas, Silvia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Buffoni Almeida, Mariana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Caruso, Mailen. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Pontoni, Andrés. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-26T11:55:22Z
2019-08-26T11:55:22Z
2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0740002019304332
http://hdl.handle.net/20.500.12123/5684
0740-0020
1095-9998
https://doi.org/10.1016/j.fm.2019.103273
url https://www.sciencedirect.com/science/article/pii/S0740002019304332
http://hdl.handle.net/20.500.12123/5684
https://doi.org/10.1016/j.fm.2019.103273
identifier_str_mv 0740-0020
1095-9998
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food Microbiology 84 : 103273 (December 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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