Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”

Autores
Blanco, Maria Evangelina; Gomez Talquenca, Gonzalo; Casassa, Luis Federico; Sari, Santiago Eduardo; Torres, Maria Del Rocio; Ulanovsky, Silvia Matilde; Fanzone, Martí­n Leandro
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin composition at harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars.
EEA Mendoza
Fil: Blanco, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina
Fil: Casassa, Luis Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
Fil: Sari, Santiago Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
Fil: Torres, María del Rocío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Ulanovsky, Silvia Matilde. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Fanzone, Martí­n Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
Fuente
Acta horticulturae 1082 : 347-352. (2015)
Materia
Genética
Secuencia Nucleotídica
Vid
Biosíntesis
Flavonoides
Genetics
Nucleotide Sequence
Grapevines
Biosynthesis
Flavonoids
Malbec
Cabernet Sauvignon
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1631
Acceder
id INTADig_7409ba01ca52968f33ec1a5f586edf07
oai_identifier_str oai:localhost:20.500.12123/1631
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”Blanco, Maria EvangelinaGomez Talquenca, GonzaloCasassa, Luis FedericoSari, Santiago EduardoTorres, Maria Del RocioUlanovsky, Silvia MatildeFanzone, Martí­n LeandroGenéticaSecuencia NucleotídicaVidBiosíntesisFlavonoidesGeneticsNucleotide SequenceGrapevinesBiosynthesisFlavonoidsMalbecCabernet SauvignonIn red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin composition at harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars.EEA MendozaFil: Blanco, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; ArgentinaFil: Casassa, Luis Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; ArgentinaFil: Sari, Santiago Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; ArgentinaFil: Torres, María del Rocío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; ArgentinaFil: Ulanovsky, Silvia Matilde. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; ArgentinaFil: Fanzone, Martí­n Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina2017-10-31T13:45:57Z2017-10-31T13:45:57Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1631http://www.actahort.org/books/1082/1082_48.htm0567-7572DOI: 10.17660/ActaHortic.2015.1082.48Acta horticulturae 1082 : 347-352. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:13Zoai:localhost:20.500.12123/1631instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:13.422INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
title Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
spellingShingle Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
Blanco, Maria Evangelina
Genética
Secuencia Nucleotídica
Vid
Biosíntesis
Flavonoides
Genetics
Nucleotide Sequence
Grapevines
Biosynthesis
Flavonoids
Malbec
Cabernet Sauvignon
title_short Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
title_full Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
title_fullStr Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
title_full_unstemmed Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
title_sort Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
dc.creator.none.fl_str_mv Blanco, Maria Evangelina
Gomez Talquenca, Gonzalo
Casassa, Luis Federico
Sari, Santiago Eduardo
Torres, Maria Del Rocio
Ulanovsky, Silvia Matilde
Fanzone, Martí­n Leandro
author Blanco, Maria Evangelina
author_facet Blanco, Maria Evangelina
Gomez Talquenca, Gonzalo
Casassa, Luis Federico
Sari, Santiago Eduardo
Torres, Maria Del Rocio
Ulanovsky, Silvia Matilde
Fanzone, Martí­n Leandro
author_role author
author2 Gomez Talquenca, Gonzalo
Casassa, Luis Federico
Sari, Santiago Eduardo
Torres, Maria Del Rocio
Ulanovsky, Silvia Matilde
Fanzone, Martí­n Leandro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Secuencia Nucleotídica
Vid
Biosíntesis
Flavonoides
Genetics
Nucleotide Sequence
Grapevines
Biosynthesis
Flavonoids
Malbec
Cabernet Sauvignon
topic Genética
Secuencia Nucleotídica
Vid
Biosíntesis
Flavonoides
Genetics
Nucleotide Sequence
Grapevines
Biosynthesis
Flavonoids
Malbec
Cabernet Sauvignon
dc.description.none.fl_txt_mv In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin composition at harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars.
EEA Mendoza
Fil: Blanco, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina
Fil: Casassa, Luis Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
Fil: Sari, Santiago Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
Fil: Torres, María del Rocío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Ulanovsky, Silvia Matilde. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina
Fil: Fanzone, Martí­n Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina
description In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin composition at harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017-10-31T13:45:57Z
2017-10-31T13:45:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1631
http://www.actahort.org/books/1082/1082_48.htm
0567-7572
DOI: 10.17660/ActaHortic.2015.1082.48
url http://hdl.handle.net/20.500.12123/1631
http://www.actahort.org/books/1082/1082_48.htm
identifier_str_mv 0567-7572
DOI: 10.17660/ActaHortic.2015.1082.48
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Acta horticulturae 1082 : 347-352. (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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