Molecular identification and characterization of two rubber dandelion amalgaviruses

Autores
Debat, Humberto Julio; Zinan Luo; Iaffaldano, Brian J.; Xiaofeng, Zhuang; Cornish, Katrina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zinan Luo. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Iaffaldano, Brian J. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Xiaofeng Zhuang. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Cornish, Katrina. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fuente
BioRxiv : the preprint server for biology. (December 5, 2017)
Materia
Taraxacum koksaghyz
Virus de las Plantas
dsRNA Viruses
Plant Viruses
Rubber Dandelion
Amalgavirus
Diente de León
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2348
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oai_identifier_str oai:localhost:20.500.12123/2348
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spelling Molecular identification and characterization of two rubber dandelion amalgavirusesDebat, Humberto JulioZinan LuoIaffaldano, Brian J.Xiaofeng, ZhuangCornish, KatrinaTaraxacum koksaghyzVirus de las PlantasdsRNA VirusesPlant VirusesRubber DandelionAmalgavirusDiente de LeónThe Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Zinan Luo. Ohio State University. Department of Horticulture and Crop Science; Estados UnidosFil: Iaffaldano, Brian J. Ohio State University. Department of Horticulture and Crop Science; Estados UnidosFil: Xiaofeng Zhuang. Ohio State University. Department of Horticulture and Crop Science; Estados UnidosFil: Cornish, Katrina. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos2018-05-08T13:34:31Z2018-05-08T13:34:31Z2017-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2348https://doi.org/10.1101/229443BioRxiv : the preprint server for biology. (December 5, 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:13Zoai:localhost:20.500.12123/2348instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:14.083INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular identification and characterization of two rubber dandelion amalgaviruses
title Molecular identification and characterization of two rubber dandelion amalgaviruses
spellingShingle Molecular identification and characterization of two rubber dandelion amalgaviruses
Debat, Humberto Julio
Taraxacum koksaghyz
Virus de las Plantas
dsRNA Viruses
Plant Viruses
Rubber Dandelion
Amalgavirus
Diente de León
title_short Molecular identification and characterization of two rubber dandelion amalgaviruses
title_full Molecular identification and characterization of two rubber dandelion amalgaviruses
title_fullStr Molecular identification and characterization of two rubber dandelion amalgaviruses
title_full_unstemmed Molecular identification and characterization of two rubber dandelion amalgaviruses
title_sort Molecular identification and characterization of two rubber dandelion amalgaviruses
dc.creator.none.fl_str_mv Debat, Humberto Julio
Zinan Luo
Iaffaldano, Brian J.
Xiaofeng, Zhuang
Cornish, Katrina
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Zinan Luo
Iaffaldano, Brian J.
Xiaofeng, Zhuang
Cornish, Katrina
author_role author
author2 Zinan Luo
Iaffaldano, Brian J.
Xiaofeng, Zhuang
Cornish, Katrina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Taraxacum koksaghyz
Virus de las Plantas
dsRNA Viruses
Plant Viruses
Rubber Dandelion
Amalgavirus
Diente de León
topic Taraxacum koksaghyz
Virus de las Plantas
dsRNA Viruses
Plant Viruses
Rubber Dandelion
Amalgavirus
Diente de León
dc.description.none.fl_txt_mv The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zinan Luo. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Iaffaldano, Brian J. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Xiaofeng Zhuang. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
Fil: Cornish, Katrina. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos
description The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-05
2018-05-08T13:34:31Z
2018-05-08T13:34:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2348
https://doi.org/10.1101/229443
url http://hdl.handle.net/20.500.12123/2348
https://doi.org/10.1101/229443
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv BioRxiv : the preprint server for biology. (December 5, 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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