Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina

Autores
Rodriguez Pardina, Patricia; Nome Docampo, Claudia; Reyna, Pablo Gastón; Muñoz, Nacira Belen; Arguello Caro, Evangelina Beatriz; Luque, Andres Vicente; Debat, Humberto Julio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina.
Instituto de Patología Vegetal
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Muñoz, Nacira Belén. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN).Cátedra de Fisiología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias (FCA). Cátedra de Zoología Agrícola,; Argentina
Fil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
BioRxiv : the preprint server for biology. (April 11, 2019)
Materia
Vicia Faba
Cordoba (Argentina)
Argentina
Potyvirus
Bean Yellow Mosaic Virus
Virions
High-Throughput Sequencing
Virus Mosaico Amarillo del Frijol
Virión
Secuenciación de Alto Rendimiento
Virus Particles
Next Generation Sequencing
Cinturón Verde de Córdoba
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12257
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12257
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, ArgentinaRodriguez Pardina, PatriciaNome Docampo, ClaudiaReyna, Pablo GastónMuñoz, Nacira BelenArguello Caro, Evangelina BeatrizLuque, Andres VicenteDebat, Humberto JulioVicia FabaCordoba (Argentina)ArgentinaPotyvirusBean Yellow Mosaic VirusVirionsHigh-Throughput SequencingVirus Mosaico Amarillo del FrijolViriónSecuenciación de Alto RendimientoVirus ParticlesNext Generation SequencingCinturón Verde de CórdobaBroad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina.Instituto de Patología VegetalFil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Muñoz, Nacira Belén. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN).Cátedra de Fisiología Vegetal; ArgentinaFil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Arguello Caro, Evangelina Beatriz. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias (FCA). Cátedra de Zoología Agrícola,; ArgentinaFil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaBioRxiv2022-07-06T11:30:34Z2022-07-06T11:30:34Z2019-04-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12257https://www.biorxiv.org/content/10.1101/606384v12692-8205https://doi.org/10.1101/606384BioRxiv : the preprint server for biology. (April 11, 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.info:eu-repograntAgreement/INTA/PNHFA-1106075/AR./Desarrollo de bases tecnológicas para el aumento de la competitividad con sostenibilidad de las Legumbres en Argentina.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:00Zoai:localhost:20.500.12123/12257instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:01.269INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
title Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
spellingShingle Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
Rodriguez Pardina, Patricia
Vicia Faba
Cordoba (Argentina)
Argentina
Potyvirus
Bean Yellow Mosaic Virus
Virions
High-Throughput Sequencing
Virus Mosaico Amarillo del Frijol
Virión
Secuenciación de Alto Rendimiento
Virus Particles
Next Generation Sequencing
Cinturón Verde de Córdoba
title_short Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
title_full Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
title_fullStr Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
title_full_unstemmed Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
title_sort Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
dc.creator.none.fl_str_mv Rodriguez Pardina, Patricia
Nome Docampo, Claudia
Reyna, Pablo Gastón
Muñoz, Nacira Belen
Arguello Caro, Evangelina Beatriz
Luque, Andres Vicente
Debat, Humberto Julio
author Rodriguez Pardina, Patricia
author_facet Rodriguez Pardina, Patricia
Nome Docampo, Claudia
Reyna, Pablo Gastón
Muñoz, Nacira Belen
Arguello Caro, Evangelina Beatriz
Luque, Andres Vicente
Debat, Humberto Julio
author_role author
author2 Nome Docampo, Claudia
Reyna, Pablo Gastón
Muñoz, Nacira Belen
Arguello Caro, Evangelina Beatriz
Luque, Andres Vicente
Debat, Humberto Julio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Vicia Faba
Cordoba (Argentina)
Argentina
Potyvirus
Bean Yellow Mosaic Virus
Virions
High-Throughput Sequencing
Virus Mosaico Amarillo del Frijol
Virión
Secuenciación de Alto Rendimiento
Virus Particles
Next Generation Sequencing
Cinturón Verde de Córdoba
topic Vicia Faba
Cordoba (Argentina)
Argentina
Potyvirus
Bean Yellow Mosaic Virus
Virions
High-Throughput Sequencing
Virus Mosaico Amarillo del Frijol
Virión
Secuenciación de Alto Rendimiento
Virus Particles
Next Generation Sequencing
Cinturón Verde de Córdoba
dc.description.none.fl_txt_mv Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina.
Instituto de Patología Vegetal
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Muñoz, Nacira Belén. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN).Cátedra de Fisiología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Arguello Caro, Evangelina Beatriz. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias (FCA). Cátedra de Zoología Agrícola,; Argentina
Fil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-11
2022-07-06T11:30:34Z
2022-07-06T11:30:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12257
https://www.biorxiv.org/content/10.1101/606384v1
2692-8205
https://doi.org/10.1101/606384
url http://hdl.handle.net/20.500.12123/12257
https://www.biorxiv.org/content/10.1101/606384v1
https://doi.org/10.1101/606384
identifier_str_mv 2692-8205
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.
info:eu-repograntAgreement/INTA/PNHFA-1106075/AR./Desarrollo de bases tecnológicas para el aumento de la competitividad con sostenibilidad de las Legumbres en Argentina.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioRxiv
publisher.none.fl_str_mv BioRxiv
dc.source.none.fl_str_mv BioRxiv : the preprint server for biology. (April 11, 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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