Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)

Autores
Fernandez, Franco Daniel; Debat, Humberto Julio; Conci, Luis Rogelio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis.
Instituto de Patología Vegetal
Fil: Fernández, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Tropical Plant Pathology 44 (4) : 392-397 (August 2019)
Materia
Argentina
Effectors
Fitoplasmas
Efectores Moleculares
Genética Molecular
Phytoplasmas
Molecular Genetics
X-disease
SAP54
16SrIII-J
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5891
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5891
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network_name_str INTA Digital (INTA)
spelling Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)Fernandez, Franco DanielDebat, Humberto JulioConci, Luis RogelioArgentinaEffectorsFitoplasmasEfectores MolecularesGenética MolecularPhytoplasmasMolecular GeneticsX-diseaseSAP5416SrIII-JPhytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis.Instituto de Patología VegetalFil: Fernández, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaSpringer2019-09-18T10:46:00Z2019-09-18T10:46:00Z2019-06-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5891https://link.springer.com/article/10.1007%2Fs40858-019-00293-01983-2052https://doi.org/10.1007/s40858-019-00293-0Tropical Plant Pathology 44 (4) : 392-397 (August 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:10Zoai:localhost:20.500.12123/5891instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:11.064INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
title Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
spellingShingle Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
Fernandez, Franco Daniel
Argentina
Effectors
Fitoplasmas
Efectores Moleculares
Genética Molecular
Phytoplasmas
Molecular Genetics
X-disease
SAP54
16SrIII-J
title_short Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
title_full Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
title_fullStr Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
title_full_unstemmed Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
title_sort Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
dc.creator.none.fl_str_mv Fernandez, Franco Daniel
Debat, Humberto Julio
Conci, Luis Rogelio
author Fernandez, Franco Daniel
author_facet Fernandez, Franco Daniel
Debat, Humberto Julio
Conci, Luis Rogelio
author_role author
author2 Debat, Humberto Julio
Conci, Luis Rogelio
author2_role author
author
dc.subject.none.fl_str_mv Argentina
Effectors
Fitoplasmas
Efectores Moleculares
Genética Molecular
Phytoplasmas
Molecular Genetics
X-disease
SAP54
16SrIII-J
topic Argentina
Effectors
Fitoplasmas
Efectores Moleculares
Genética Molecular
Phytoplasmas
Molecular Genetics
X-disease
SAP54
16SrIII-J
dc.description.none.fl_txt_mv Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis.
Instituto de Patología Vegetal
Fil: Fernández, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-18T10:46:00Z
2019-09-18T10:46:00Z
2019-06-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5891
https://link.springer.com/article/10.1007%2Fs40858-019-00293-0
1983-2052
https://doi.org/10.1007/s40858-019-00293-0
url http://hdl.handle.net/20.500.12123/5891
https://link.springer.com/article/10.1007%2Fs40858-019-00293-0
https://doi.org/10.1007/s40858-019-00293-0
identifier_str_mv 1983-2052
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical Plant Pathology 44 (4) : 392-397 (August 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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