Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
- Autores
- Tortone, Claudia Andrea; Zumarraga, Martin Jose; Gioffre, Andrea; Oriani, Delia Susana
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.
Instituto de Biotecnología
Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina - Fuente
- International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)
- Materia
-
Enzymatic Analysis
Waterborne Diseases
Análisis Enzimático
Enfermedades Transmitidas por Agua
Nontuberculous Mycobacteria
Phenotypic Methods
Water Sources
Micobacterias no Tuberculosas
Métodos Fenotípicos
Fuentes de Agua - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/3625
Ver los metadatos del registro completo
id |
INTADig_6a9b505dae34b1426ef53e6ae80015a2 |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/3625 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sourcesTortone, Claudia AndreaZumarraga, Martin JoseGioffre, AndreaOriani, Delia SusanaEnzymatic AnalysisWaterborne DiseasesAnálisis EnzimáticoEnfermedades Transmitidas por AguaNontuberculous MycobacteriaPhenotypic MethodsWater SourcesMicobacterias no TuberculosasMétodos FenotípicosFuentes de AguaBackground: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.Instituto de BiotecnologíaFil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaWolters Kluwer - Medknow2018-10-18T12:48:22Z2018-10-18T12:48:22Z2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortonehttp://hdl.handle.net/20.500.12123/36252212-5531DOI: 10.4103/ijmy.ijmy_192_17International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:27Zoai:localhost:20.500.12123/3625instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:27.8INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
title |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
spellingShingle |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources Tortone, Claudia Andrea Enzymatic Analysis Waterborne Diseases Análisis Enzimático Enfermedades Transmitidas por Agua Nontuberculous Mycobacteria Phenotypic Methods Water Sources Micobacterias no Tuberculosas Métodos Fenotípicos Fuentes de Agua |
title_short |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
title_full |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
title_fullStr |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
title_full_unstemmed |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
title_sort |
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources |
dc.creator.none.fl_str_mv |
Tortone, Claudia Andrea Zumarraga, Martin Jose Gioffre, Andrea Oriani, Delia Susana |
author |
Tortone, Claudia Andrea |
author_facet |
Tortone, Claudia Andrea Zumarraga, Martin Jose Gioffre, Andrea Oriani, Delia Susana |
author_role |
author |
author2 |
Zumarraga, Martin Jose Gioffre, Andrea Oriani, Delia Susana |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Enzymatic Analysis Waterborne Diseases Análisis Enzimático Enfermedades Transmitidas por Agua Nontuberculous Mycobacteria Phenotypic Methods Water Sources Micobacterias no Tuberculosas Métodos Fenotípicos Fuentes de Agua |
topic |
Enzymatic Analysis Waterborne Diseases Análisis Enzimático Enfermedades Transmitidas por Agua Nontuberculous Mycobacteria Phenotypic Methods Water Sources Micobacterias no Tuberculosas Métodos Fenotípicos Fuentes de Agua |
dc.description.none.fl_txt_mv |
Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods. Instituto de Biotecnología Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina |
description |
Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-18T12:48:22Z 2018-10-18T12:48:22Z 2018-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortone http://hdl.handle.net/20.500.12123/3625 2212-5531 DOI: 10.4103/ijmy.ijmy_192_17 |
url |
http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortone http://hdl.handle.net/20.500.12123/3625 |
identifier_str_mv |
2212-5531 DOI: 10.4103/ijmy.ijmy_192_17 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wolters Kluwer - Medknow |
publisher.none.fl_str_mv |
Wolters Kluwer - Medknow |
dc.source.none.fl_str_mv |
International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1844619126841016320 |
score |
12.559606 |