Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources

Autores
Tortone, Claudia Andrea; Zumarraga, Martin Jose; Gioffre, Andrea; Oriani, Delia Susana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.
Instituto de Biotecnología
Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fuente
International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)
Materia
Enzymatic Analysis
Waterborne Diseases
Análisis Enzimático
Enfermedades Transmitidas por Agua
Nontuberculous Mycobacteria
Phenotypic Methods
Water Sources
Micobacterias no Tuberculosas
Métodos Fenotípicos
Fuentes de Agua
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3625
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oai_identifier_str oai:localhost:20.500.12123/3625
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network_name_str INTA Digital (INTA)
spelling Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sourcesTortone, Claudia AndreaZumarraga, Martin JoseGioffre, AndreaOriani, Delia SusanaEnzymatic AnalysisWaterborne DiseasesAnálisis EnzimáticoEnfermedades Transmitidas por AguaNontuberculous MycobacteriaPhenotypic MethodsWater SourcesMicobacterias no TuberculosasMétodos FenotípicosFuentes de AguaBackground: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.Instituto de BiotecnologíaFil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; ArgentinaWolters Kluwer - Medknow2018-10-18T12:48:22Z2018-10-18T12:48:22Z2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortonehttp://hdl.handle.net/20.500.12123/36252212-5531DOI: 10.4103/ijmy.ijmy_192_17International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:41Zoai:localhost:20.500.12123/3625instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:41.725INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
title Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
spellingShingle Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
Tortone, Claudia Andrea
Enzymatic Analysis
Waterborne Diseases
Análisis Enzimático
Enfermedades Transmitidas por Agua
Nontuberculous Mycobacteria
Phenotypic Methods
Water Sources
Micobacterias no Tuberculosas
Métodos Fenotípicos
Fuentes de Agua
title_short Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
title_full Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
title_fullStr Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
title_full_unstemmed Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
title_sort Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
dc.creator.none.fl_str_mv Tortone, Claudia Andrea
Zumarraga, Martin Jose
Gioffre, Andrea
Oriani, Delia Susana
author Tortone, Claudia Andrea
author_facet Tortone, Claudia Andrea
Zumarraga, Martin Jose
Gioffre, Andrea
Oriani, Delia Susana
author_role author
author2 Zumarraga, Martin Jose
Gioffre, Andrea
Oriani, Delia Susana
author2_role author
author
author
dc.subject.none.fl_str_mv Enzymatic Analysis
Waterborne Diseases
Análisis Enzimático
Enfermedades Transmitidas por Agua
Nontuberculous Mycobacteria
Phenotypic Methods
Water Sources
Micobacterias no Tuberculosas
Métodos Fenotípicos
Fuentes de Agua
topic Enzymatic Analysis
Waterborne Diseases
Análisis Enzimático
Enfermedades Transmitidas por Agua
Nontuberculous Mycobacteria
Phenotypic Methods
Water Sources
Micobacterias no Tuberculosas
Métodos Fenotípicos
Fuentes de Agua
dc.description.none.fl_txt_mv Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.
Instituto de Biotecnología
Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
description Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-18T12:48:22Z
2018-10-18T12:48:22Z
2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortone
http://hdl.handle.net/20.500.12123/3625
2212-5531
DOI: 10.4103/ijmy.ijmy_192_17
url http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortone
http://hdl.handle.net/20.500.12123/3625
identifier_str_mv 2212-5531
DOI: 10.4103/ijmy.ijmy_192_17
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer - Medknow
publisher.none.fl_str_mv Wolters Kluwer - Medknow
dc.source.none.fl_str_mv International Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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