A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle

Autores
Verdes, José Manuel; Márquez, Mercedes; Calliari, Aldo; Battes, Daniel; Moraña, José Antonio; Gimeno, Eduardo Juan; Odriozola, Ernesto Raul; Giannitti, Federico; Guerrero, Florentina; Fidalgo, Luis Eusebio; Pumarola, Martí
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
EEA Balcarce
Fil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
Fil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; Argentina
Fil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; Uruguay
Fil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; España
Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; España
Fil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
Fuente
Journal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015)
Materia
Ganado Bovino
Enfermedades de los Animales
Envenenamiento
Solanum
Cattle
Animal Diseases
Poisoning
Intoxicación
Solanum bonariense
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4834
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oai_identifier_str oai:localhost:20.500.12123/4834
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattleVerdes, José ManuelMárquez, MercedesCalliari, AldoBattes, DanielMoraña, José AntonioGimeno, Eduardo JuanOdriozola, Ernesto RaulGiannitti, FedericoGuerrero, FlorentinaFidalgo, Luis EusebioPumarola, MartíGanado BovinoEnfermedades de los AnimalesEnvenenamientoSolanumCattleAnimal DiseasesPoisoningIntoxicaciónSolanum bonarienseIntoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.EEA BalcarceFil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; UruguayFil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; EspañaFil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; UruguayFil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay.Fil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay.Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; ArgentinaFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; UruguayFil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; EspañaFil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; EspañaFil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; EspañaAmerican Association of Veterinary Laboratory Diagnosticians2019-04-08T11:48:36Z2019-04-08T11:48:36Z2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/48341040-63871943-4936https://doi.org/10.1177/1040638715582048Journal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:55Zoai:localhost:20.500.12123/4834instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:55.676INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
title A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
spellingShingle A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
Verdes, José Manuel
Ganado Bovino
Enfermedades de los Animales
Envenenamiento
Solanum
Cattle
Animal Diseases
Poisoning
Intoxicación
Solanum bonariense
title_short A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
title_full A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
title_fullStr A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
title_full_unstemmed A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
title_sort A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle
dc.creator.none.fl_str_mv Verdes, José Manuel
Márquez, Mercedes
Calliari, Aldo
Battes, Daniel
Moraña, José Antonio
Gimeno, Eduardo Juan
Odriozola, Ernesto Raul
Giannitti, Federico
Guerrero, Florentina
Fidalgo, Luis Eusebio
Pumarola, Martí
author Verdes, José Manuel
author_facet Verdes, José Manuel
Márquez, Mercedes
Calliari, Aldo
Battes, Daniel
Moraña, José Antonio
Gimeno, Eduardo Juan
Odriozola, Ernesto Raul
Giannitti, Federico
Guerrero, Florentina
Fidalgo, Luis Eusebio
Pumarola, Martí
author_role author
author2 Márquez, Mercedes
Calliari, Aldo
Battes, Daniel
Moraña, José Antonio
Gimeno, Eduardo Juan
Odriozola, Ernesto Raul
Giannitti, Federico
Guerrero, Florentina
Fidalgo, Luis Eusebio
Pumarola, Martí
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Envenenamiento
Solanum
Cattle
Animal Diseases
Poisoning
Intoxicación
Solanum bonariense
topic Ganado Bovino
Enfermedades de los Animales
Envenenamiento
Solanum
Cattle
Animal Diseases
Poisoning
Intoxicación
Solanum bonariense
dc.description.none.fl_txt_mv Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
EEA Balcarce
Fil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
Fil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay
Fil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; Argentina
Fil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; Uruguay
Fil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; España
Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; España
Fil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; España
description Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
2019-04-08T11:48:36Z
2019-04-08T11:48:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4834
1040-6387
1943-4936
https://doi.org/10.1177/1040638715582048
url http://hdl.handle.net/20.500.12123/4834
https://doi.org/10.1177/1040638715582048
identifier_str_mv 1040-6387
1943-4936
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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