In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates

Autores
Imperiale, Belen Rocio; Cataldi, Angel Adrian; Morcillo, Nora
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments. Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions. The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method.
Inst. de Biotecnología
Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fuente
Revista Argentina de Microbiología 49 (4) : 332-338 (October - December 2017)
Materia
Mycobacterium
Resistencia a Medicamentos
Azoles
Mycobacterium Tuberculosis
Imidazoles
Experimentación in Vitro
Drug Resistance
In Vitro Experimentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1961
Acceder
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spelling In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolatesImperiale, Belen RocioCataldi, Angel AdrianMorcillo, NoraMycobacteriumResistencia a MedicamentosAzolesMycobacterium TuberculosisImidazolesExperimentación in VitroDrug ResistanceIn Vitro ExperimentationBackground: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments. Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions. The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method.Inst. de BiotecnologíaFil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina2018-03-05T15:41:50Z2018-03-05T15:41:50Z2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1961http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895http://dx.doi.org/10.1016/j.ram.2017.02.008Revista Argentina de Microbiología 49 (4) : 332-338 (October - December 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:27Zoai:localhost:20.500.12123/1961instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:28.354INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
title In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
spellingShingle In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
Imperiale, Belen Rocio
Mycobacterium
Resistencia a Medicamentos
Azoles
Mycobacterium Tuberculosis
Imidazoles
Experimentación in Vitro
Drug Resistance
In Vitro Experimentation
title_short In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
title_full In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
title_fullStr In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
title_full_unstemmed In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
title_sort In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
dc.creator.none.fl_str_mv Imperiale, Belen Rocio
Cataldi, Angel Adrian
Morcillo, Nora
author Imperiale, Belen Rocio
author_facet Imperiale, Belen Rocio
Cataldi, Angel Adrian
Morcillo, Nora
author_role author
author2 Cataldi, Angel Adrian
Morcillo, Nora
author2_role author
author
dc.subject.none.fl_str_mv Mycobacterium
Resistencia a Medicamentos
Azoles
Mycobacterium Tuberculosis
Imidazoles
Experimentación in Vitro
Drug Resistance
In Vitro Experimentation
topic Mycobacterium
Resistencia a Medicamentos
Azoles
Mycobacterium Tuberculosis
Imidazoles
Experimentación in Vitro
Drug Resistance
In Vitro Experimentation
dc.description.none.fl_txt_mv Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments. Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions. The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method.
Inst. de Biotecnología
Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
description Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments. Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions. The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2018-03-05T15:41:50Z
2018-03-05T15:41:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1961
http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895
http://dx.doi.org/10.1016/j.ram.2017.02.008
url http://hdl.handle.net/20.500.12123/1961
http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895
http://dx.doi.org/10.1016/j.ram.2017.02.008
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Revista Argentina de Microbiología 49 (4) : 332-338 (October - December 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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