Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina

Autores
Vaghi Medina, Carlos Gaston; Teppa, Elin; Bornancini, Veronica Andrea; Flores, Ceferino Rene; Marino Buslje, Cristina; Lopez Lambertini, Paola Maria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.
INTA. CIAP. Instituto de Patología Vegetal
Fil: Vaghi Medina, Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil:Teppa, Elin. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina
Fil: Bornancini, Verónica A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Flores, Ceferino R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Marino Buslje, Cristina. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina
Fil: López Lambertini, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Frontiers in Microbiology January 2018
Materia
Solanum lycopersicum
Tomate
Coat Proteins
Recombination
Tomato
Sequence Similarity Network
Treehopper
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2169
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2169
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in ArgentinaVaghi Medina, Carlos GastonTeppa, ElinBornancini, Veronica AndreaFlores, Ceferino ReneMarino Buslje, CristinaLopez Lambertini, Paola MariaSolanum lycopersicumTomateCoat ProteinsRecombinationTomatoSequence Similarity NetworkTreehopperPlant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.INTA. CIAP. Instituto de Patología VegetalFil: Vaghi Medina, Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil:Teppa, Elin. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; ArgentinaFil: Bornancini, Verónica A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Flores, Ceferino R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Marino Buslje, Cristina. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; ArgentinaFil: López Lambertini, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina2018-04-04T18:24:45Z2018-04-04T18:24:45Z2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/fullhttp://hdl.handle.net/20.500.12123/21691664-302Xhttps://doi.org/10.3389/fmicb.2017.02665Frontiers in Microbiology January 2018reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:11Zoai:localhost:20.500.12123/2169instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:11.992INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
title Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
spellingShingle Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
Vaghi Medina, Carlos Gaston
Solanum lycopersicum
Tomate
Coat Proteins
Recombination
Tomato
Sequence Similarity Network
Treehopper
title_short Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
title_full Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
title_fullStr Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
title_full_unstemmed Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
title_sort Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
dc.creator.none.fl_str_mv Vaghi Medina, Carlos Gaston
Teppa, Elin
Bornancini, Veronica Andrea
Flores, Ceferino Rene
Marino Buslje, Cristina
Lopez Lambertini, Paola Maria
author Vaghi Medina, Carlos Gaston
author_facet Vaghi Medina, Carlos Gaston
Teppa, Elin
Bornancini, Veronica Andrea
Flores, Ceferino Rene
Marino Buslje, Cristina
Lopez Lambertini, Paola Maria
author_role author
author2 Teppa, Elin
Bornancini, Veronica Andrea
Flores, Ceferino Rene
Marino Buslje, Cristina
Lopez Lambertini, Paola Maria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Solanum lycopersicum
Tomate
Coat Proteins
Recombination
Tomato
Sequence Similarity Network
Treehopper
topic Solanum lycopersicum
Tomate
Coat Proteins
Recombination
Tomato
Sequence Similarity Network
Treehopper
dc.description.none.fl_txt_mv Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.
INTA. CIAP. Instituto de Patología Vegetal
Fil: Vaghi Medina, Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil:Teppa, Elin. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina
Fil: Bornancini, Verónica A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Flores, Ceferino R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Marino Buslje, Cristina. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina
Fil: López Lambertini, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-04T18:24:45Z
2018-04-04T18:24:45Z
2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full
http://hdl.handle.net/20.500.12123/2169
1664-302X
https://doi.org/10.3389/fmicb.2017.02665
url https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full
http://hdl.handle.net/20.500.12123/2169
https://doi.org/10.3389/fmicb.2017.02665
identifier_str_mv 1664-302X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Frontiers in Microbiology January 2018
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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