Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species

Autores
Paz, Rosalia Cristina; Rendina González, Alejandra Pilar; Ferrer, M.S.; Masuelli, Ricardo Williams
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation.
EEA La Consulta
Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan. Departamento de Biología. Grupo INTERBIODES; Argentina
Fil: Rendina González, Alejandra Pilar. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Ferrer, M.S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fuente
Plant Biology 17 (4) : 860-869 (July 2015)
Materia
Solanum
Hibridación Interespecífica
Variación Genética
Transposones
Genética
Interspecific Hybridization
Genetic Variation
Transposons
Genetics
Elementos Genéticos Transponibles
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3066
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3066
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum speciesPaz, Rosalia CristinaRendina González, Alejandra PilarFerrer, M.S.Masuelli, Ricardo WilliamsSolanumHibridación InterespecíficaVariación GenéticaTransposonesGenéticaInterspecific HybridizationGenetic VariationTransposonsGeneticsElementos Genéticos TransponiblesInterspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation.EEA La ConsultaFil: Paz, Rosalia Cristina. Universidad Nacional de San Juan. Departamento de Biología. Grupo INTERBIODES; ArgentinaFil: Rendina González, Alejandra Pilar. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Ferrer, M.S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina2018-08-14T14:42:31Z2018-08-14T14:42:31Z2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301http://hdl.handle.net/20.500.12123/30661435-86031438-8677https://doi.org/10.1111/plb.12301Plant Biology 17 (4) : 860-869 (July 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:16Zoai:localhost:20.500.12123/3066instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:16.612INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
title Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
spellingShingle Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
Paz, Rosalia Cristina
Solanum
Hibridación Interespecífica
Variación Genética
Transposones
Genética
Interspecific Hybridization
Genetic Variation
Transposons
Genetics
Elementos Genéticos Transponibles
title_short Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
title_full Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
title_fullStr Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
title_full_unstemmed Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
title_sort Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
dc.creator.none.fl_str_mv Paz, Rosalia Cristina
Rendina González, Alejandra Pilar
Ferrer, M.S.
Masuelli, Ricardo Williams
author Paz, Rosalia Cristina
author_facet Paz, Rosalia Cristina
Rendina González, Alejandra Pilar
Ferrer, M.S.
Masuelli, Ricardo Williams
author_role author
author2 Rendina González, Alejandra Pilar
Ferrer, M.S.
Masuelli, Ricardo Williams
author2_role author
author
author
dc.subject.none.fl_str_mv Solanum
Hibridación Interespecífica
Variación Genética
Transposones
Genética
Interspecific Hybridization
Genetic Variation
Transposons
Genetics
Elementos Genéticos Transponibles
topic Solanum
Hibridación Interespecífica
Variación Genética
Transposones
Genética
Interspecific Hybridization
Genetic Variation
Transposons
Genetics
Elementos Genéticos Transponibles
dc.description.none.fl_txt_mv Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation.
EEA La Consulta
Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan. Departamento de Biología. Grupo INTERBIODES; Argentina
Fil: Rendina González, Alejandra Pilar. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Ferrer, M.S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
description Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2018-08-14T14:42:31Z
2018-08-14T14:42:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301
http://hdl.handle.net/20.500.12123/3066
1435-8603
1438-8677
https://doi.org/10.1111/plb.12301
url https://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301
http://hdl.handle.net/20.500.12123/3066
https://doi.org/10.1111/plb.12301
identifier_str_mv 1435-8603
1438-8677
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Biology 17 (4) : 860-869 (July 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.229304

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