Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs

Autores: Ospina Calvo, Brian; De Geronimo, Eduardo; Villarruel, Fernando D.; Aparicio, Virginia Carolina; Ashworth, Lorena; Erra Balsells, Rosa; Cabrerizo, Franco M.
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: This study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs of Passiflora caerulea. UHPLC–MS/MS data reported herein reveal that the βCs' content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs' content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs' content also varied in a structure-dependent manner. Alkaloids bearing a hydroxyl group at position C(7) of the main βC ring were present at concentrations one order of magnitude higher than other βC derivatives investigated. UV–visible and fluorescence spectroscopy of the flower extracts provided complementary information regarding other biologically relevant groups of chromophores (phenolic/indolic derivatives, flavonoids/carotenes, and chlorophylls). Since flowers are constantly exposed to solar radiation, the presence of photoactive βCs in floral organs may have several (photo) biological implications that are further discussed.
EEA Balcarce
Fil: Ospina Calvo, Brian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina
Fil: De Gerónimo, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: De Gerónimo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Villarruel, Fernando D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina
Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Aparicio, Virginia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Ashworth, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Ashworth, Lorena. Universidad Nacional de Córdoba (UNC). Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Departamento de Química Orgánica. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono (CIHIDECAR); Argentina
Fil: Rosa Erra Balsells. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación en Hidratos de Carbono (CIHIDECAR); Argentina
Fil: Cabrerizo, Franco M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina
Fil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina
Fuente: Photochemistry and Photobiology 100 (1) : 87-100. (January/February 2024)
Materia: Spectroscopy
Alkaloids
Photosensitivity
Espectroscopia
Alcaloide
Fotosensibilidad
Fluorescence Spectroscopy
Absorption Spectroscopy
Floral Organs
Espectroscopia de Fluorescencia
Espectroscopia de Absorción
Passiflora caerulea
Órganos Florales
Nivel de accesibilidad: acceso restringido
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/25473

Acceder

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spelling	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organsOspina Calvo, BrianDe Geronimo, EduardoVillarruel, Fernando D.Aparicio, Virginia CarolinaAshworth, LorenaErra Balsells, RosaCabrerizo, Franco M.SpectroscopyAlkaloidsPhotosensitivityEspectroscopiaAlcaloideFotosensibilidadFluorescence SpectroscopyAbsorption SpectroscopyFloral OrgansEspectroscopia de FluorescenciaEspectroscopia de AbsorciónPassiflora caeruleaÓrganos FloralesThis study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs of Passiflora caerulea. UHPLC–MS/MS data reported herein reveal that the βCs' content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs' content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs' content also varied in a structure-dependent manner. Alkaloids bearing a hydroxyl group at position C(7) of the main βC ring were present at concentrations one order of magnitude higher than other βC derivatives investigated. UV–visible and fluorescence spectroscopy of the flower extracts provided complementary information regarding other biologically relevant groups of chromophores (phenolic/indolic derivatives, flavonoids/carotenes, and chlorophylls). Since flowers are constantly exposed to solar radiation, the presence of photoactive βCs in floral organs may have several (photo) biological implications that are further discussed.EEA BalcarceFil: Ospina Calvo, Brian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; ArgentinaFil: De Gerónimo, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: De Gerónimo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Villarruel, Fernando D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; ArgentinaFil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Aparicio, Virginia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Ashworth, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Ashworth, Lorena. Universidad Nacional de Córdoba (UNC). Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Departamento de Química Orgánica. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono (CIHIDECAR); ArgentinaFil: Rosa Erra Balsells. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación en Hidratos de Carbono (CIHIDECAR); ArgentinaFil: Cabrerizo, Franco M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); ArgentinaFil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; ArgentinaWiley2026-03-13T16:52:25Z2026-03-13T16:52:25Z2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25473https://onlinelibrary.wiley.com/doi/10.1111/php.138371751-10970031-8655https://doi.org/10.1111/php.13837Photochemistry and Photobiology 100 (1) : 87-100. (January/February 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-03-26T11:25:30Zoai:localhost:20.500.12123/25473instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-03-26 11:25:31.062INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
title	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
spellingShingle	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs Ospina Calvo, Brian Spectroscopy Alkaloids Photosensitivity Espectroscopia Alcaloide Fotosensibilidad Fluorescence Spectroscopy Absorption Spectroscopy Floral Organs Espectroscopia de Fluorescencia Espectroscopia de Absorción Passiflora caerulea Órganos Florales
title_short	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
title_full	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
title_fullStr	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
title_full_unstemmed	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
title_sort	Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs
dc.creator.none.fl_str_mv	Ospina Calvo, Brian De Geronimo, Eduardo Villarruel, Fernando D. Aparicio, Virginia Carolina Ashworth, Lorena Erra Balsells, Rosa Cabrerizo, Franco M.
author	Ospina Calvo, Brian
author_facet	Ospina Calvo, Brian De Geronimo, Eduardo Villarruel, Fernando D. Aparicio, Virginia Carolina Ashworth, Lorena Erra Balsells, Rosa Cabrerizo, Franco M.
author_role	author
author2	De Geronimo, Eduardo Villarruel, Fernando D. Aparicio, Virginia Carolina Ashworth, Lorena Erra Balsells, Rosa Cabrerizo, Franco M.
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Spectroscopy Alkaloids Photosensitivity Espectroscopia Alcaloide Fotosensibilidad Fluorescence Spectroscopy Absorption Spectroscopy Floral Organs Espectroscopia de Fluorescencia Espectroscopia de Absorción Passiflora caerulea Órganos Florales
topic	Spectroscopy Alkaloids Photosensitivity Espectroscopia Alcaloide Fotosensibilidad Fluorescence Spectroscopy Absorption Spectroscopy Floral Organs Espectroscopia de Fluorescencia Espectroscopia de Absorción Passiflora caerulea Órganos Florales
dc.description.none.fl_txt_mv	This study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs of Passiflora caerulea. UHPLC–MS/MS data reported herein reveal that the βCs' content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs' content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs' content also varied in a structure-dependent manner. Alkaloids bearing a hydroxyl group at position C(7) of the main βC ring were present at concentrations one order of magnitude higher than other βC derivatives investigated. UV–visible and fluorescence spectroscopy of the flower extracts provided complementary information regarding other biologically relevant groups of chromophores (phenolic/indolic derivatives, flavonoids/carotenes, and chlorophylls). Since flowers are constantly exposed to solar radiation, the presence of photoactive βCs in floral organs may have several (photo) biological implications that are further discussed. EEA Balcarce Fil: Ospina Calvo, Brian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Ospina Calvo, Brian. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina Fil: De Gerónimo, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: De Gerónimo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Villarruel, Fernando D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Villarruel, Fernando D. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Aparicio, Virginia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Ashworth, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina Fil: Ashworth, Lorena. Universidad Nacional de Córdoba (UNC). Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina Fil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Departamento de Química Orgánica. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Rosa Erra Balsells. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono (CIHIDECAR); Argentina Fil: Rosa Erra Balsells. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación en Hidratos de Carbono (CIHIDECAR); Argentina Fil: Cabrerizo, Franco M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Instituto Tecnológico de Chascomús (INTECH); Argentina Fil: Cabrerizo, Franco M. Universidad Nacional de San Martín (UNSAM). Escuela de Bio y Nanotecnologías; Argentina
description	This study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs of Passiflora caerulea. UHPLC–MS/MS data reported herein reveal that the βCs' content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs' content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs' content also varied in a structure-dependent manner. Alkaloids bearing a hydroxyl group at position C(7) of the main βC ring were present at concentrations one order of magnitude higher than other βC derivatives investigated. UV–visible and fluorescence spectroscopy of the flower extracts provided complementary information regarding other biologically relevant groups of chromophores (phenolic/indolic derivatives, flavonoids/carotenes, and chlorophylls). Since flowers are constantly exposed to solar radiation, the presence of photoactive βCs in floral organs may have several (photo) biological implications that are further discussed.
publishDate	2024
dc.date.none.fl_str_mv	2024-02 2026-03-13T16:52:25Z 2026-03-13T16:52:25Z
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
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dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/25473 https://onlinelibrary.wiley.com/doi/10.1111/php.13837 1751-1097 0031-8655 https://doi.org/10.1111/php.13837
url	http://hdl.handle.net/20.500.12123/25473 https://onlinelibrary.wiley.com/doi/10.1111/php.13837 https://doi.org/10.1111/php.13837
identifier_str_mv	1751-1097 0031-8655
dc.language.none.fl_str_mv	eng
language	eng
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rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	Photochemistry and Photobiology 100 (1) : 87-100. (January/February 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
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repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Distribution of photoactive β- carboline alkaloids across Passiflora caerulea floral organs

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