Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples

Autores
Zumarraga, Martin Jose; Soutullo, Adriana; Garcia, Maria Ines; Marini, M. Rocío; Abdala, Alejandro Ariel; Tarabla, Hector Dante; Echaide, Susana T. de; Lopez, Marcela; Zervini, Elsa; Canal, Ana María; Cataldi, Angel Adrian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis.
Instituto de Biotecnología
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Soutullo, Adriana. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina
Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Inmunología Básica; Argentina
Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina
Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Echaide, Susana T. de. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lopez, Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Zervini, Elsa. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Canal, Ana María. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Foodborne Pathogens and Disease 9 (2) : 132-137 (Febrero 2012)
Materia
Mycobacterium bovis
Rebaño de Leche
PCR
Leche
Tuberculosis
Argentina
Dairy Herds
Milk
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8317
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8317
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samplesZumarraga, Martin JoseSoutullo, AdrianaGarcia, Maria InesMarini, M. RocíoAbdala, Alejandro ArielTarabla, Hector DanteEchaide, Susana T. deLopez, MarcelaZervini, ElsaCanal, Ana MaríaCataldi, Angel AdrianMycobacterium bovisRebaño de LechePCRLecheTuberculosisArgentinaDairy HerdsMilkBovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis.Instituto de BiotecnologíaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Soutullo, Adriana. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; ArgentinaFil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Inmunología Básica; ArgentinaFil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; ArgentinaFil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Echaide, Susana T. de. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Lopez, Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaFil: Zervini, Elsa. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; ArgentinaFil: Canal, Ana María. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaMary Ann Liebert Inc.2020-11-24T16:44:41Z2020-11-24T16:44:41Z2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8317https://www.liebertpub.com/doi/10.1089/fpd.2011.09631556-7125http://doi.org/10.1089/fpd.2011.0963Foodborne Pathogens and Disease 9 (2) : 132-137 (Febrero 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:42Zoai:localhost:20.500.12123/8317instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:43.128INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
title Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
spellingShingle Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
Zumarraga, Martin Jose
Mycobacterium bovis
Rebaño de Leche
PCR
Leche
Tuberculosis
Argentina
Dairy Herds
Milk
title_short Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
title_full Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
title_fullStr Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
title_full_unstemmed Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
title_sort Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
dc.creator.none.fl_str_mv Zumarraga, Martin Jose
Soutullo, Adriana
Garcia, Maria Ines
Marini, M. Rocío
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Echaide, Susana T. de
Lopez, Marcela
Zervini, Elsa
Canal, Ana María
Cataldi, Angel Adrian
author Zumarraga, Martin Jose
author_facet Zumarraga, Martin Jose
Soutullo, Adriana
Garcia, Maria Ines
Marini, M. Rocío
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Echaide, Susana T. de
Lopez, Marcela
Zervini, Elsa
Canal, Ana María
Cataldi, Angel Adrian
author_role author
author2 Soutullo, Adriana
Garcia, Maria Ines
Marini, M. Rocío
Abdala, Alejandro Ariel
Tarabla, Hector Dante
Echaide, Susana T. de
Lopez, Marcela
Zervini, Elsa
Canal, Ana María
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
Rebaño de Leche
PCR
Leche
Tuberculosis
Argentina
Dairy Herds
Milk
topic Mycobacterium bovis
Rebaño de Leche
PCR
Leche
Tuberculosis
Argentina
Dairy Herds
Milk
dc.description.none.fl_txt_mv Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis.
Instituto de Biotecnología
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Soutullo, Adriana. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina
Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Inmunología Básica; Argentina
Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina
Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Echaide, Susana T. de. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lopez, Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Zervini, Elsa. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina
Fil: Canal, Ana María. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
2020-11-24T16:44:41Z
2020-11-24T16:44:41Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8317
https://www.liebertpub.com/doi/10.1089/fpd.2011.0963
1556-7125
http://doi.org/10.1089/fpd.2011.0963
url http://hdl.handle.net/20.500.12123/8317
https://www.liebertpub.com/doi/10.1089/fpd.2011.0963
http://doi.org/10.1089/fpd.2011.0963
identifier_str_mv 1556-7125
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc.
publisher.none.fl_str_mv Mary Ann Liebert Inc.
dc.source.none.fl_str_mv Foodborne Pathogens and Disease 9 (2) : 132-137 (Febrero 2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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