Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification

Autores
Majul, Leonardo; Topalian, Juliana; Omarini, Alejandra; Levin, Laura; Campos, Eleonora; Wirth, Sonia Alejandra
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Developing processes to valorize underutilized agri-food side streams is essential for an efficient circular bioeconomy. A promising strategy is their use as low-cost carbon sources for microbial fermentation to produce industrially relevant molecules. In this study, wheat bran, rice bran, and rice husk served as substrates for generating carbohydrase-rich cocktails via submerged fermentation with Pycnoporus sanguineus BAFC 2126 and Trametes trogii BAFC 463. Both fungi produced enzyme extracts rich in cellulase and xylanase activities, while P. sanguineus also showed α-L-arabinofuranosidase and α-D-galactosidase activities. Wheat bran and rice husk were the most effective inducers for carbohydrase expression in P. sanguineus and T. trogii, respectively. Notably, the enzyme extract from P. sanguineus grown on wheat bran (PsWB) showed thermostable enzymatic activity, with a half-life of over 2 h at 60 °C. In addition, secretome analysis of PsWB revealed a complete set of hydrolytic cellulases, as well as diverse hemicellulases, pectinases, peptidases, and laccases. In contrast, the secretome of T. trogii grown on rice husk (TtRH) showed lower enzymatic diversity. Nevertheless, in biomass hydrolysis assays, TtRH showed higher glucan-to-glucose conversion efficiency for both wheat bran and corn-soybean-based broiler feed (44% and 69%, respectively), while PsWB was more effective in xylan-to-xylose conversion (25% and 34%, respectively). Altogether, these findings highlight the potential of PsWB and TtRH enzyme cocktails as feed additives for monogastric animals. This study also presents the first comprehensive characterization of the secretome of P. sanguineus and T. trogii when cultivated on wheat bran and rice husk, respectively.
Instituto de Biotecnología
Fil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Majul, Leonardo. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Majul, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Topalian, Juliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Omarini, Alejandra. Asociación para el Desarrollo de Villa Elisa y Zona; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA); Argentina
Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Biocatalysis and Agricultural Biotechnology 73 : 104023 (April 2026)
Materia
Lignocellulose
Circular Bioeconomy
Poultry Feeding
Pycnoporus
Trametes
Cereals
Carbohydrases
Hydrolysis
Fermentation
Lignocelulosa
Bioeconomía Circular
Alimentación Avícola
Cereales
Carbohidrasa
Hidrólisis
Fermentación
Pycnoporus sanguineus
Trametes trogii
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26064
Acceder
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oai_identifier_str oai:localhost:20.500.12123/26064
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharificationMajul, LeonardoTopalian, JulianaOmarini, AlejandraLevin, LauraCampos, EleonoraWirth, Sonia AlejandraLignocelluloseCircular BioeconomyPoultry FeedingPycnoporusTrametesCerealsCarbohydrasesHydrolysisFermentationLignocelulosaBioeconomía CircularAlimentación AvícolaCerealesCarbohidrasaHidrólisisFermentaciónPycnoporus sanguineusTrametes trogiiDeveloping processes to valorize underutilized agri-food side streams is essential for an efficient circular bioeconomy. A promising strategy is their use as low-cost carbon sources for microbial fermentation to produce industrially relevant molecules. In this study, wheat bran, rice bran, and rice husk served as substrates for generating carbohydrase-rich cocktails via submerged fermentation with Pycnoporus sanguineus BAFC 2126 and Trametes trogii BAFC 463. Both fungi produced enzyme extracts rich in cellulase and xylanase activities, while P. sanguineus also showed α-L-arabinofuranosidase and α-D-galactosidase activities. Wheat bran and rice husk were the most effective inducers for carbohydrase expression in P. sanguineus and T. trogii, respectively. Notably, the enzyme extract from P. sanguineus grown on wheat bran (PsWB) showed thermostable enzymatic activity, with a half-life of over 2 h at 60 °C. In addition, secretome analysis of PsWB revealed a complete set of hydrolytic cellulases, as well as diverse hemicellulases, pectinases, peptidases, and laccases. In contrast, the secretome of T. trogii grown on rice husk (TtRH) showed lower enzymatic diversity. Nevertheless, in biomass hydrolysis assays, TtRH showed higher glucan-to-glucose conversion efficiency for both wheat bran and corn-soybean-based broiler feed (44% and 69%, respectively), while PsWB was more effective in xylan-to-xylose conversion (25% and 34%, respectively). Altogether, these findings highlight the potential of PsWB and TtRH enzyme cocktails as feed additives for monogastric animals. This study also presents the first comprehensive characterization of the secretome of P. sanguineus and T. trogii when cultivated on wheat bran and rice husk, respectively.Instituto de BiotecnologíaFil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; ArgentinaFil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; ArgentinaFil: Majul, Leonardo. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); ArgentinaFil: Majul, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); ArgentinaFil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Topalian, Juliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Omarini, Alejandra. Asociación para el Desarrollo de Villa Elisa y Zona; ArgentinaFil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; ArgentinaFil: Levin, Laura. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); ArgentinaFil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; ArgentinaFil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA); ArgentinaFil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2026-05-06T12:34:46Z2026-05-06T12:34:46Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26064https://www.sciencedirect.com/science/article/abs/pii/S18788181260010641878-8181https://doi.org/10.1016/j.bcab.2026.104023Biocatalysis and Agricultural Biotechnology 73 : 104023 (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-05-07T11:53:22Zoai:localhost:20.500.12123/26064instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-07 11:53:22.467INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
title Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
spellingShingle Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
Majul, Leonardo
Lignocellulose
Circular Bioeconomy
Poultry Feeding
Pycnoporus
Trametes
Cereals
Carbohydrases
Hydrolysis
Fermentation
Lignocelulosa
Bioeconomía Circular
Alimentación Avícola
Cereales
Carbohidrasa
Hidrólisis
Fermentación
Pycnoporus sanguineus
Trametes trogii
title_short Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
title_full Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
title_fullStr Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
title_full_unstemmed Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
title_sort Valorization of cereal processing side-streams for carbohydrase production by white-rot fungi and application in feed saccharification
dc.creator.none.fl_str_mv Majul, Leonardo
Topalian, Juliana
Omarini, Alejandra
Levin, Laura
Campos, Eleonora
Wirth, Sonia Alejandra
author Majul, Leonardo
author_facet Majul, Leonardo
Topalian, Juliana
Omarini, Alejandra
Levin, Laura
Campos, Eleonora
Wirth, Sonia Alejandra
author_role author
author2 Topalian, Juliana
Omarini, Alejandra
Levin, Laura
Campos, Eleonora
Wirth, Sonia Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Lignocellulose
Circular Bioeconomy
Poultry Feeding
Pycnoporus
Trametes
Cereals
Carbohydrases
Hydrolysis
Fermentation
Lignocelulosa
Bioeconomía Circular
Alimentación Avícola
Cereales
Carbohidrasa
Hidrólisis
Fermentación
Pycnoporus sanguineus
Trametes trogii
topic Lignocellulose
Circular Bioeconomy
Poultry Feeding
Pycnoporus
Trametes
Cereals
Carbohydrases
Hydrolysis
Fermentation
Lignocelulosa
Bioeconomía Circular
Alimentación Avícola
Cereales
Carbohidrasa
Hidrólisis
Fermentación
Pycnoporus sanguineus
Trametes trogii
dc.description.none.fl_txt_mv Developing processes to valorize underutilized agri-food side streams is essential for an efficient circular bioeconomy. A promising strategy is their use as low-cost carbon sources for microbial fermentation to produce industrially relevant molecules. In this study, wheat bran, rice bran, and rice husk served as substrates for generating carbohydrase-rich cocktails via submerged fermentation with Pycnoporus sanguineus BAFC 2126 and Trametes trogii BAFC 463. Both fungi produced enzyme extracts rich in cellulase and xylanase activities, while P. sanguineus also showed α-L-arabinofuranosidase and α-D-galactosidase activities. Wheat bran and rice husk were the most effective inducers for carbohydrase expression in P. sanguineus and T. trogii, respectively. Notably, the enzyme extract from P. sanguineus grown on wheat bran (PsWB) showed thermostable enzymatic activity, with a half-life of over 2 h at 60 °C. In addition, secretome analysis of PsWB revealed a complete set of hydrolytic cellulases, as well as diverse hemicellulases, pectinases, peptidases, and laccases. In contrast, the secretome of T. trogii grown on rice husk (TtRH) showed lower enzymatic diversity. Nevertheless, in biomass hydrolysis assays, TtRH showed higher glucan-to-glucose conversion efficiency for both wheat bran and corn-soybean-based broiler feed (44% and 69%, respectively), while PsWB was more effective in xylan-to-xylose conversion (25% and 34%, respectively). Altogether, these findings highlight the potential of PsWB and TtRH enzyme cocktails as feed additives for monogastric animals. This study also presents the first comprehensive characterization of the secretome of P. sanguineus and T. trogii when cultivated on wheat bran and rice husk, respectively.
Instituto de Biotecnología
Fil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Majul, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina
Fil: Majul, Leonardo. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Majul, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Topalian, Juliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Omarini, Alejandra. Asociación para el Desarrollo de Villa Elisa y Zona; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina
Fil: Levin, Laura. Universidad de Buenos Aires. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica (INMIBO); Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA); Argentina
Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Developing processes to valorize underutilized agri-food side streams is essential for an efficient circular bioeconomy. A promising strategy is their use as low-cost carbon sources for microbial fermentation to produce industrially relevant molecules. In this study, wheat bran, rice bran, and rice husk served as substrates for generating carbohydrase-rich cocktails via submerged fermentation with Pycnoporus sanguineus BAFC 2126 and Trametes trogii BAFC 463. Both fungi produced enzyme extracts rich in cellulase and xylanase activities, while P. sanguineus also showed α-L-arabinofuranosidase and α-D-galactosidase activities. Wheat bran and rice husk were the most effective inducers for carbohydrase expression in P. sanguineus and T. trogii, respectively. Notably, the enzyme extract from P. sanguineus grown on wheat bran (PsWB) showed thermostable enzymatic activity, with a half-life of over 2 h at 60 °C. In addition, secretome analysis of PsWB revealed a complete set of hydrolytic cellulases, as well as diverse hemicellulases, pectinases, peptidases, and laccases. In contrast, the secretome of T. trogii grown on rice husk (TtRH) showed lower enzymatic diversity. Nevertheless, in biomass hydrolysis assays, TtRH showed higher glucan-to-glucose conversion efficiency for both wheat bran and corn-soybean-based broiler feed (44% and 69%, respectively), while PsWB was more effective in xylan-to-xylose conversion (25% and 34%, respectively). Altogether, these findings highlight the potential of PsWB and TtRH enzyme cocktails as feed additives for monogastric animals. This study also presents the first comprehensive characterization of the secretome of P. sanguineus and T. trogii when cultivated on wheat bran and rice husk, respectively.
publishDate 2026
dc.date.none.fl_str_mv 2026-05-06T12:34:46Z
2026-05-06T12:34:46Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26064
https://www.sciencedirect.com/science/article/abs/pii/S1878818126001064
1878-8181
https://doi.org/10.1016/j.bcab.2026.104023
url http://hdl.handle.net/20.500.12123/26064
https://www.sciencedirect.com/science/article/abs/pii/S1878818126001064
https://doi.org/10.1016/j.bcab.2026.104023
identifier_str_mv 1878-8181
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biocatalysis and Agricultural Biotechnology 73 : 104023 (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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