Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of spe...

Autores: Fígoli, Cecilia Beatriz; Rojo, Rodrigo; Gasoni, Amelia Laura; Kikot, Gisele Eleonora; Leguizamón, Mariana; Gamba, Raúl Ricardo; Bosch, Alejandra; Alconada Magliano, Teresa Maria
Año de publicación: 2017
Idioma: inglés
Tipo de recurso: artículo
Estado: versión aceptada
Descripción: Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis.
Inst. de Microbiología y Zoología Agrícola IMyZA
Fil: Fígoli, Cecilia Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina
Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Leguizamón, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina
Fil: Gamba, Raul Ricardo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Bosch, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina
Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fuente: International journal of food microbiology 244 : 36-42. (March 2017)
Materia: Enfermedades de las Plantas
Gibberella Zeae
Trigo
Fenotipos
Espectroscopía
Plant Diseases
Wheat
Phenotypes
Spectroscopy
Fusarium Graminearum
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/1514

Acceder

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spelling	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressivenessFígoli, Cecilia BeatrizRojo, RodrigoGasoni, Amelia LauraKikot, Gisele EleonoraLeguizamón, MarianaGamba, Raúl RicardoBosch, AlejandraAlconada Magliano, Teresa MariaEnfermedades de las PlantasGibberella ZeaeTrigoFenotiposEspectroscopíaPlant DiseasesWheatPhenotypesSpectroscopyFusarium GraminearumFusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis.Inst. de Microbiología y Zoología Agrícola IMyZAFil: Fígoli, Cecilia Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; ArgentinaFil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Leguizamón, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; ArgentinaFil: Gamba, Raul Ricardo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Bosch, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; ArgentinaFil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina2017-10-18T13:00:18Z2017-10-18T13:00:18Z2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1514http://www.sciencedirect.com/science/article/pii/S016816051630664X0168-1605 (Print)1879-3460 (Online)https://doi.org/10.1016/j.ijfoodmicro.2016.12.016International journal of food microbiology 244 : 36-42. (March 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-09-04T09:47:06Zoai:localhost:20.500.12123/1514instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:07.053INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
title	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
spellingShingle	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness Fígoli, Cecilia Beatriz Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum
title_short	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
title_full	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
title_fullStr	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
title_full_unstemmed	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
title_sort	Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
dc.creator.none.fl_str_mv	Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria
author	Fígoli, Cecilia Beatriz
author_facet	Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria
author_role	author
author2	Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum
topic	Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum
dc.description.none.fl_txt_mv	Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Fígoli, Cecilia Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina Fil: Leguizamón, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Gamba, Raul Ricardo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Bosch, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description	Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis.
publishDate	2017
dc.date.none.fl_str_mv	2017-10-18T13:00:18Z 2017-10-18T13:00:18Z 2017-03
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/1514 http://www.sciencedirect.com/science/article/pii/S016816051630664X 0168-1605 (Print) 1879-3460 (Online) https://doi.org/10.1016/j.ijfoodmicro.2016.12.016
url	http://hdl.handle.net/20.500.12123/1514 http://www.sciencedirect.com/science/article/pii/S016816051630664X https://doi.org/10.1016/j.ijfoodmicro.2016.12.016
identifier_str_mv	0168-1605 (Print) 1879-3460 (Online)
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv	restrictedAccess
dc.format.none.fl_str_mv	application/pdf
dc.coverage.none.fl_str_mv	Argentina (nation)
dc.source.none.fl_str_mv	International journal of food microbiology 244 : 36-42. (March 2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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score	12.623145

Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of spe...

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