Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks

Autores
Frydman, Camila Ayelén; Parreño, Gladys Viviana; Cap, Mariana; Galeano, Fabiana Solange; Signorini Porchiett, Marcelo Lisandro; Mozgovoj, Marina Valeria
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foodborne viral infections are a global public health concern, yet quantitative risk assessments (QMRA) for enteric viruses in bivalve mollusks are scarce, especially in South America. We conducted Argentina's first QMRA for five human enteric viruses- norovirus (NoV), rotavirus A (RVA), hepatitis A (HAV), hepatitis E (HEV), and adenovirus (AdV) in bivalve mollusks. From August 2018–March 2023, 390 specimens from Golfo Nuevo, Chubut, were pooled (n = 113) and analyzed by real time PCR. A national survey revealed 3.6 % of servings were raw or minimally cooked. AdV showed the highest prevalence and viral load, followed by NoV GII and RVA; HAV and HEV had lower loads, and NoV GI was undetected. Per-serving infection risks peaked for AdV and NoV GII from raw oyster and mussel consumption. Individual annual infection probabilities were 2.44 × 10−1 for AdV, 1.33 × 10−1 for NoV GII, and 6.60 × 10−2 for HAV. RVA and HEV posed lower risks. Sensitivity analysis identified AdV and HAV prevalence, and mussel and clam consumption, as key drivers of the shellfish-associated infection risk. Our findings pinpoint raw and undercooked oysters and mussels as significant sources of risk. This QMRA provides crucial, country-specific evidence to support optimizing disinfection processes and enhancing monitoring of critical environmental contamination sources along the seafood production and distribution chain.
Instituto Tecnología de Alimentos (ITA)
Fil: Frydman, Camila Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Frydman, Camila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Frydman, Camila Ayelén. Universidad Nacional de Hurlingham (UNAHUR); Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Parreño, Gladys Viviana. Universidad Nacional de Hurlingham (UNAHUR); Argentina
Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Cap, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Galeano, Fabiana Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Galeano, Fabiana Solange. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Signorini Porchiett, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Signorini Porchiett, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Mozgovoj, Marina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Mozgovoj, Marina Valeria. Universidad Nacional de Hurlingham (UNAHUR); Argentina
Fuente
Food Microbiology 135 : 104981. (April 2026)
Materia
Shellfish
Viruses
Food Contamination
Microbial Contamination
Marisco
Virus
Contaminación Alimentaria
Contaminación Microbiana
Argentine Bivalve Mollusks
Moluscos Bivalvos Argentinos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25000
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oai_identifier_str oai:localhost:20.500.12123/25000
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusksFrydman, Camila AyelénParreño, Gladys VivianaCap, MarianaGaleano, Fabiana SolangeSignorini Porchiett, Marcelo LisandroMozgovoj, Marina ValeriaShellfishVirusesFood ContaminationMicrobial ContaminationMariscoVirusContaminación AlimentariaContaminación MicrobianaArgentine Bivalve MollusksMoluscos Bivalvos ArgentinosFoodborne viral infections are a global public health concern, yet quantitative risk assessments (QMRA) for enteric viruses in bivalve mollusks are scarce, especially in South America. We conducted Argentina's first QMRA for five human enteric viruses- norovirus (NoV), rotavirus A (RVA), hepatitis A (HAV), hepatitis E (HEV), and adenovirus (AdV) in bivalve mollusks. From August 2018–March 2023, 390 specimens from Golfo Nuevo, Chubut, were pooled (n = 113) and analyzed by real time PCR. A national survey revealed 3.6 % of servings were raw or minimally cooked. AdV showed the highest prevalence and viral load, followed by NoV GII and RVA; HAV and HEV had lower loads, and NoV GI was undetected. Per-serving infection risks peaked for AdV and NoV GII from raw oyster and mussel consumption. Individual annual infection probabilities were 2.44 × 10−1 for AdV, 1.33 × 10−1 for NoV GII, and 6.60 × 10−2 for HAV. RVA and HEV posed lower risks. Sensitivity analysis identified AdV and HAV prevalence, and mussel and clam consumption, as key drivers of the shellfish-associated infection risk. Our findings pinpoint raw and undercooked oysters and mussels as significant sources of risk. This QMRA provides crucial, country-specific evidence to support optimizing disinfection processes and enhancing monitoring of critical environmental contamination sources along the seafood production and distribution chain.Instituto Tecnología de Alimentos (ITA)Fil: Frydman, Camila Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); ArgentinaFil: Frydman, Camila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); ArgentinaFil: Frydman, Camila Ayelén. Universidad Nacional de Hurlingham (UNAHUR); ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Parreño, Gladys Viviana. Universidad Nacional de Hurlingham (UNAHUR); ArgentinaFil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); ArgentinaFil: Cap, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); ArgentinaFil: Galeano, Fabiana Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); ArgentinaFil: Galeano, Fabiana Solange. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); ArgentinaFil: Signorini Porchiett, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Signorini Porchiett, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); ArgentinaFil: Mozgovoj, Marina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); ArgentinaFil: Mozgovoj, Marina Valeria. Universidad Nacional de Hurlingham (UNAHUR); ArgentinaElsevier2026-01-16T18:21:53Z2026-01-16T18:21:53Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25000https://www.sciencedirect.com/science/article/abs/pii/S0740002025002631?via%3Dihub0740-0020 (impreso)1095-9998 (en línea)https://doi.org/10.1016/j.fm.2025.104981Food Microbiology 135 : 104981. (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengPatagonia .......... (general region) (World, South America, Argentina)7016766info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:41Zoai:localhost:20.500.12123/25000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:41.407INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
title Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
spellingShingle Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
Frydman, Camila Ayelén
Shellfish
Viruses
Food Contamination
Microbial Contamination
Marisco
Virus
Contaminación Alimentaria
Contaminación Microbiana
Argentine Bivalve Mollusks
Moluscos Bivalvos Argentinos
title_short Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
title_full Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
title_fullStr Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
title_full_unstemmed Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
title_sort Patagonian shellfish and hidden threats: unveiling the viral landscape and the first quantitative microbial risk assessment of Argentine bivalve mollusks
dc.creator.none.fl_str_mv Frydman, Camila Ayelén
Parreño, Gladys Viviana
Cap, Mariana
Galeano, Fabiana Solange
Signorini Porchiett, Marcelo Lisandro
Mozgovoj, Marina Valeria
author Frydman, Camila Ayelén
author_facet Frydman, Camila Ayelén
Parreño, Gladys Viviana
Cap, Mariana
Galeano, Fabiana Solange
Signorini Porchiett, Marcelo Lisandro
Mozgovoj, Marina Valeria
author_role author
author2 Parreño, Gladys Viviana
Cap, Mariana
Galeano, Fabiana Solange
Signorini Porchiett, Marcelo Lisandro
Mozgovoj, Marina Valeria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Shellfish
Viruses
Food Contamination
Microbial Contamination
Marisco
Virus
Contaminación Alimentaria
Contaminación Microbiana
Argentine Bivalve Mollusks
Moluscos Bivalvos Argentinos
topic Shellfish
Viruses
Food Contamination
Microbial Contamination
Marisco
Virus
Contaminación Alimentaria
Contaminación Microbiana
Argentine Bivalve Mollusks
Moluscos Bivalvos Argentinos
dc.description.none.fl_txt_mv Foodborne viral infections are a global public health concern, yet quantitative risk assessments (QMRA) for enteric viruses in bivalve mollusks are scarce, especially in South America. We conducted Argentina's first QMRA for five human enteric viruses- norovirus (NoV), rotavirus A (RVA), hepatitis A (HAV), hepatitis E (HEV), and adenovirus (AdV) in bivalve mollusks. From August 2018–March 2023, 390 specimens from Golfo Nuevo, Chubut, were pooled (n = 113) and analyzed by real time PCR. A national survey revealed 3.6 % of servings were raw or minimally cooked. AdV showed the highest prevalence and viral load, followed by NoV GII and RVA; HAV and HEV had lower loads, and NoV GI was undetected. Per-serving infection risks peaked for AdV and NoV GII from raw oyster and mussel consumption. Individual annual infection probabilities were 2.44 × 10−1 for AdV, 1.33 × 10−1 for NoV GII, and 6.60 × 10−2 for HAV. RVA and HEV posed lower risks. Sensitivity analysis identified AdV and HAV prevalence, and mussel and clam consumption, as key drivers of the shellfish-associated infection risk. Our findings pinpoint raw and undercooked oysters and mussels as significant sources of risk. This QMRA provides crucial, country-specific evidence to support optimizing disinfection processes and enhancing monitoring of critical environmental contamination sources along the seafood production and distribution chain.
Instituto Tecnología de Alimentos (ITA)
Fil: Frydman, Camila Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Frydman, Camila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Frydman, Camila Ayelén. Universidad Nacional de Hurlingham (UNAHUR); Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Parreño, Gladys Viviana. Universidad Nacional de Hurlingham (UNAHUR); Argentina
Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Cap, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Galeano, Fabiana Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Galeano, Fabiana Solange. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Signorini Porchiett, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Signorini Porchiett, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos (ITA); Argentina
Fil: Mozgovoj, Marina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS); Argentina
Fil: Mozgovoj, Marina Valeria. Universidad Nacional de Hurlingham (UNAHUR); Argentina
description Foodborne viral infections are a global public health concern, yet quantitative risk assessments (QMRA) for enteric viruses in bivalve mollusks are scarce, especially in South America. We conducted Argentina's first QMRA for five human enteric viruses- norovirus (NoV), rotavirus A (RVA), hepatitis A (HAV), hepatitis E (HEV), and adenovirus (AdV) in bivalve mollusks. From August 2018–March 2023, 390 specimens from Golfo Nuevo, Chubut, were pooled (n = 113) and analyzed by real time PCR. A national survey revealed 3.6 % of servings were raw or minimally cooked. AdV showed the highest prevalence and viral load, followed by NoV GII and RVA; HAV and HEV had lower loads, and NoV GI was undetected. Per-serving infection risks peaked for AdV and NoV GII from raw oyster and mussel consumption. Individual annual infection probabilities were 2.44 × 10−1 for AdV, 1.33 × 10−1 for NoV GII, and 6.60 × 10−2 for HAV. RVA and HEV posed lower risks. Sensitivity analysis identified AdV and HAV prevalence, and mussel and clam consumption, as key drivers of the shellfish-associated infection risk. Our findings pinpoint raw and undercooked oysters and mussels as significant sources of risk. This QMRA provides crucial, country-specific evidence to support optimizing disinfection processes and enhancing monitoring of critical environmental contamination sources along the seafood production and distribution chain.
publishDate 2026
dc.date.none.fl_str_mv 2026-01-16T18:21:53Z
2026-01-16T18:21:53Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25000
https://www.sciencedirect.com/science/article/abs/pii/S0740002025002631?via%3Dihub
0740-0020 (impreso)
1095-9998 (en línea)
https://doi.org/10.1016/j.fm.2025.104981
url http://hdl.handle.net/20.500.12123/25000
https://www.sciencedirect.com/science/article/abs/pii/S0740002025002631?via%3Dihub
https://doi.org/10.1016/j.fm.2025.104981
identifier_str_mv 0740-0020 (impreso)
1095-9998 (en línea)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Patagonia .......... (general region) (World, South America, Argentina)
7016766
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food Microbiology 135 : 104981. (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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