Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding

Autores
Cornacchione, Monica; Odorizzi, Ariel; Basigalup, Daniel Horacio; Arolfo, Valeria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poster y resumen
A new cultivar with increased salinity tolerance was obtained from the INTA alfalfa genetic breeding program. The Medicago sativa germplasm (Synthetic Santiago=SISA 14) is a salt-tolerant synthetic population derived from three cycles of phenotypic recurrent selection under a semiarid saline natural condition at the Isla Verde, Santiago del Estero, Argentina (28°38’41.9’’S, 64°05’03.8’’W; soil taxonomy: typic Natracualf). The parental material is derived mainly from cv. Salado (fall dormancy=FD 9), with minor contributions from AZ-97MEC-ST and AZ-97MEC populations (FD 9). The best genotypes at each cycle were selected based on the ability to emerge, grow, and produce forage and seeds under a natural condition of medium to moderate high saline soil. The saline site where the seeds germinated and emerged was the same in the three selection cycles, having an electrical conductivity of saturated paste extract (ECex 0-5cm) between 10.6 to 32.8 dS m-1 and average pH of 7.74 (a minimum amount of water was applied to moisten the soil surface). Selection in cycle I (March 2007): 250 seedlings were selected after emergence. Each seedling was transplanted temporarily to an individual small bag. Later, each plant was transplanted directly to the soil in a nearby paddock (ECex (0-30cm) oscillated from 4.1 to 20.8 dS m-1 between April to November, respectively; with increasing salinity in depth) within the same field with the proper isolation to preserve its genetic purity. Plants were grown and intercross until December. The seeds were harvested from selected plants for good vigor, large size, lack of foliar disease and bulked to form the first selection cycle. In March 2008, these seeds were sown in the germination site described before, and a new selection was done after seedling emergence. Thereafter, it followed the same procedure previously described. The site in where second intercross cycle was made had ECex (0-30cm) oscillated between a maximum of 32.2 in September 2008 to a minimum of 19 dS m-1 in March 2009. Only 70 genotypes were selected to produce the intercrossing seeds. Seed harvests were done in December 2008 and March 2009 and bulked to form the second selection cycle. These seeds were sown in March of 2010 and 84 seedlings were selected and following the same procedure as before. The site in where third intercross cycle was made had ECex (0-30 cm) 14 dS m-1 in average. Seed harvests were done in December 2010 and March 2011, and bulked to form the third selection cycle. These seeds becoming the breeder seeds or Syn-1. Intercross where made by native effective pollinators bees Xylocopa spp. As it was mentioned, the soil salinity is rarely spatially uniform in field conditions and each of the three cycles has been done under a different level of saline stress. Thus, a long-term study was carried out under controlled conditions of temperature, light and different concentrations and type of salts in the facilities of the USDA Salinity Laboratory, Riverside USA (2011-2012). The irrigation system and closed drainage utilized allow maintaining the levels of stress constant, which allowed lead a comparative evaluation of several alfalfa populations. SISA 14 (Syn-1) combined high tolerance (in relative terms) and higher biomass production (gr pl-1 ), explained in part by a lower concentration of Na+ in its aerial biomass as one of the attributes associated with one of the mechanisms contributing to the tolerance in alfalfa (Cornacchione and Suarez, 2017). The Syn-1 seeds were sent to the EEA INTA San Juan, Argentina in the fall of 2011 and Syn-2 seed was obtained in April 2012. Subsequently, the Syn-2 was sent in April 2015 and the Syn-3 was obtained in 2016. Syn-2 were used to conformed (in Argentina) evaluation trials of agronomic behavior sowed on fall of 2015 and conducted for 3 seasons (2015/2016, 2016/2017 and 2017/2018) at two locations (EEA Manfredi, EEA Santiago del Estero) and one season at the EEA Rafaela (2015/2016). From Syn-3, the population was conducted as a synthetic cultivar.
EEA Manfredi
Fil: Cornacchione, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Basigalup, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fuente
Proceedings of the Second World Alfalfa Congress Global Interaction for Alfalfa Innovation. Cordoba, Agentina, 11-14 noviembre, 2018, p. 178
Materia
Medicago sativa
Salinidad
Mejoramiento Genético
Argentina
Salinity
Genetic Improvement
Salt Tolerance
Tolerancia a la Sal
Alfalfa
Selección Recurrente Fenotípica
Lucerne
Phenotypic Recurrent Selection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18862

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oai_identifier_str oai:localhost:20.500.12123/18862
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spelling Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breedingCornacchione, MonicaOdorizzi, ArielBasigalup, Daniel HoracioArolfo, ValeriaMedicago sativaSalinidadMejoramiento GenéticoArgentinaSalinityGenetic ImprovementSalt ToleranceTolerancia a la SalAlfalfaSelección Recurrente FenotípicaLucernePhenotypic Recurrent SelectionPoster y resumenA new cultivar with increased salinity tolerance was obtained from the INTA alfalfa genetic breeding program. The Medicago sativa germplasm (Synthetic Santiago=SISA 14) is a salt-tolerant synthetic population derived from three cycles of phenotypic recurrent selection under a semiarid saline natural condition at the Isla Verde, Santiago del Estero, Argentina (28°38’41.9’’S, 64°05’03.8’’W; soil taxonomy: typic Natracualf). The parental material is derived mainly from cv. Salado (fall dormancy=FD 9), with minor contributions from AZ-97MEC-ST and AZ-97MEC populations (FD 9). The best genotypes at each cycle were selected based on the ability to emerge, grow, and produce forage and seeds under a natural condition of medium to moderate high saline soil. The saline site where the seeds germinated and emerged was the same in the three selection cycles, having an electrical conductivity of saturated paste extract (ECex 0-5cm) between 10.6 to 32.8 dS m-1 and average pH of 7.74 (a minimum amount of water was applied to moisten the soil surface). Selection in cycle I (March 2007): 250 seedlings were selected after emergence. Each seedling was transplanted temporarily to an individual small bag. Later, each plant was transplanted directly to the soil in a nearby paddock (ECex (0-30cm) oscillated from 4.1 to 20.8 dS m-1 between April to November, respectively; with increasing salinity in depth) within the same field with the proper isolation to preserve its genetic purity. Plants were grown and intercross until December. The seeds were harvested from selected plants for good vigor, large size, lack of foliar disease and bulked to form the first selection cycle. In March 2008, these seeds were sown in the germination site described before, and a new selection was done after seedling emergence. Thereafter, it followed the same procedure previously described. The site in where second intercross cycle was made had ECex (0-30cm) oscillated between a maximum of 32.2 in September 2008 to a minimum of 19 dS m-1 in March 2009. Only 70 genotypes were selected to produce the intercrossing seeds. Seed harvests were done in December 2008 and March 2009 and bulked to form the second selection cycle. These seeds were sown in March of 2010 and 84 seedlings were selected and following the same procedure as before. The site in where third intercross cycle was made had ECex (0-30 cm) 14 dS m-1 in average. Seed harvests were done in December 2010 and March 2011, and bulked to form the third selection cycle. These seeds becoming the breeder seeds or Syn-1. Intercross where made by native effective pollinators bees Xylocopa spp. As it was mentioned, the soil salinity is rarely spatially uniform in field conditions and each of the three cycles has been done under a different level of saline stress. Thus, a long-term study was carried out under controlled conditions of temperature, light and different concentrations and type of salts in the facilities of the USDA Salinity Laboratory, Riverside USA (2011-2012). The irrigation system and closed drainage utilized allow maintaining the levels of stress constant, which allowed lead a comparative evaluation of several alfalfa populations. SISA 14 (Syn-1) combined high tolerance (in relative terms) and higher biomass production (gr pl-1 ), explained in part by a lower concentration of Na+ in its aerial biomass as one of the attributes associated with one of the mechanisms contributing to the tolerance in alfalfa (Cornacchione and Suarez, 2017). The Syn-1 seeds were sent to the EEA INTA San Juan, Argentina in the fall of 2011 and Syn-2 seed was obtained in April 2012. Subsequently, the Syn-2 was sent in April 2015 and the Syn-3 was obtained in 2016. Syn-2 were used to conformed (in Argentina) evaluation trials of agronomic behavior sowed on fall of 2015 and conducted for 3 seasons (2015/2016, 2016/2017 and 2017/2018) at two locations (EEA Manfredi, EEA Santiago del Estero) and one season at the EEA Rafaela (2015/2016). From Syn-3, the population was conducted as a synthetic cultivar.EEA ManfrediFil: Cornacchione, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; ArgentinaFil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Basigalup, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaINTA2024-08-08T11:00:41Z2024-08-08T11:00:41Z2018-11-11info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/18862http://www.worldalfalfacongress.org/resumenes/?lang=enProceedings of the Second World Alfalfa Congress Global Interaction for Alfalfa Innovation. Cordoba, Agentina, 11-14 noviembre, 2018, p. 178reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/CORDO/1262205/AR./Proyecto regional del territorio agrícola ganadero central de la provincia de Córdoba.info:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.info:eu-repograntAgreement/INTA/PNPA/1126072/AR./Desarrollo de cultivares superiores de especies forrajeras para sistemas ganaderos y agricolo-ganaderos de la Argentina.info:eu-repograntAgreement/INTA/CORDO/1262204/AR./Gestión de la innovación en el territorio del arco noroeste de la provincia de Córdoba.info:eu-repograntAgreement/INTA/CORDO/1262206/AR./PReT - Zona III centro Este - Centro Regional Córdoba.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:42Zoai:localhost:20.500.12123/18862instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:42.842INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
title Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
spellingShingle Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
Cornacchione, Monica
Medicago sativa
Salinidad
Mejoramiento Genético
Argentina
Salinity
Genetic Improvement
Salt Tolerance
Tolerancia a la Sal
Alfalfa
Selección Recurrente Fenotípica
Lucerne
Phenotypic Recurrent Selection
title_short Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
title_full Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
title_fullStr Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
title_full_unstemmed Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
title_sort Improving salinity tolerance of alfalfa (Medicago sativa L.) through conventional breeding
dc.creator.none.fl_str_mv Cornacchione, Monica
Odorizzi, Ariel
Basigalup, Daniel Horacio
Arolfo, Valeria
author Cornacchione, Monica
author_facet Cornacchione, Monica
Odorizzi, Ariel
Basigalup, Daniel Horacio
Arolfo, Valeria
author_role author
author2 Odorizzi, Ariel
Basigalup, Daniel Horacio
Arolfo, Valeria
author2_role author
author
author
dc.subject.none.fl_str_mv Medicago sativa
Salinidad
Mejoramiento Genético
Argentina
Salinity
Genetic Improvement
Salt Tolerance
Tolerancia a la Sal
Alfalfa
Selección Recurrente Fenotípica
Lucerne
Phenotypic Recurrent Selection
topic Medicago sativa
Salinidad
Mejoramiento Genético
Argentina
Salinity
Genetic Improvement
Salt Tolerance
Tolerancia a la Sal
Alfalfa
Selección Recurrente Fenotípica
Lucerne
Phenotypic Recurrent Selection
dc.description.none.fl_txt_mv Poster y resumen
A new cultivar with increased salinity tolerance was obtained from the INTA alfalfa genetic breeding program. The Medicago sativa germplasm (Synthetic Santiago=SISA 14) is a salt-tolerant synthetic population derived from three cycles of phenotypic recurrent selection under a semiarid saline natural condition at the Isla Verde, Santiago del Estero, Argentina (28°38’41.9’’S, 64°05’03.8’’W; soil taxonomy: typic Natracualf). The parental material is derived mainly from cv. Salado (fall dormancy=FD 9), with minor contributions from AZ-97MEC-ST and AZ-97MEC populations (FD 9). The best genotypes at each cycle were selected based on the ability to emerge, grow, and produce forage and seeds under a natural condition of medium to moderate high saline soil. The saline site where the seeds germinated and emerged was the same in the three selection cycles, having an electrical conductivity of saturated paste extract (ECex 0-5cm) between 10.6 to 32.8 dS m-1 and average pH of 7.74 (a minimum amount of water was applied to moisten the soil surface). Selection in cycle I (March 2007): 250 seedlings were selected after emergence. Each seedling was transplanted temporarily to an individual small bag. Later, each plant was transplanted directly to the soil in a nearby paddock (ECex (0-30cm) oscillated from 4.1 to 20.8 dS m-1 between April to November, respectively; with increasing salinity in depth) within the same field with the proper isolation to preserve its genetic purity. Plants were grown and intercross until December. The seeds were harvested from selected plants for good vigor, large size, lack of foliar disease and bulked to form the first selection cycle. In March 2008, these seeds were sown in the germination site described before, and a new selection was done after seedling emergence. Thereafter, it followed the same procedure previously described. The site in where second intercross cycle was made had ECex (0-30cm) oscillated between a maximum of 32.2 in September 2008 to a minimum of 19 dS m-1 in March 2009. Only 70 genotypes were selected to produce the intercrossing seeds. Seed harvests were done in December 2008 and March 2009 and bulked to form the second selection cycle. These seeds were sown in March of 2010 and 84 seedlings were selected and following the same procedure as before. The site in where third intercross cycle was made had ECex (0-30 cm) 14 dS m-1 in average. Seed harvests were done in December 2010 and March 2011, and bulked to form the third selection cycle. These seeds becoming the breeder seeds or Syn-1. Intercross where made by native effective pollinators bees Xylocopa spp. As it was mentioned, the soil salinity is rarely spatially uniform in field conditions and each of the three cycles has been done under a different level of saline stress. Thus, a long-term study was carried out under controlled conditions of temperature, light and different concentrations and type of salts in the facilities of the USDA Salinity Laboratory, Riverside USA (2011-2012). The irrigation system and closed drainage utilized allow maintaining the levels of stress constant, which allowed lead a comparative evaluation of several alfalfa populations. SISA 14 (Syn-1) combined high tolerance (in relative terms) and higher biomass production (gr pl-1 ), explained in part by a lower concentration of Na+ in its aerial biomass as one of the attributes associated with one of the mechanisms contributing to the tolerance in alfalfa (Cornacchione and Suarez, 2017). The Syn-1 seeds were sent to the EEA INTA San Juan, Argentina in the fall of 2011 and Syn-2 seed was obtained in April 2012. Subsequently, the Syn-2 was sent in April 2015 and the Syn-3 was obtained in 2016. Syn-2 were used to conformed (in Argentina) evaluation trials of agronomic behavior sowed on fall of 2015 and conducted for 3 seasons (2015/2016, 2016/2017 and 2017/2018) at two locations (EEA Manfredi, EEA Santiago del Estero) and one season at the EEA Rafaela (2015/2016). From Syn-3, the population was conducted as a synthetic cultivar.
EEA Manfredi
Fil: Cornacchione, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Basigalup, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
description Poster y resumen
publishDate 2018
dc.date.none.fl_str_mv 2018-11-11
2024-08-08T11:00:41Z
2024-08-08T11:00:41Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18862
http://www.worldalfalfacongress.org/resumenes/?lang=en
url http://hdl.handle.net/20.500.12123/18862
http://www.worldalfalfacongress.org/resumenes/?lang=en
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/CORDO/1262205/AR./Proyecto regional del territorio agrícola ganadero central de la provincia de Córdoba.
info:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.
info:eu-repograntAgreement/INTA/PNPA/1126072/AR./Desarrollo de cultivares superiores de especies forrajeras para sistemas ganaderos y agricolo-ganaderos de la Argentina.
info:eu-repograntAgreement/INTA/CORDO/1262204/AR./Gestión de la innovación en el territorio del arco noroeste de la provincia de Córdoba.
info:eu-repograntAgreement/INTA/CORDO/1262206/AR./PReT - Zona III centro Este - Centro Regional Córdoba.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv INTA
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dc.source.none.fl_str_mv Proceedings of the Second World Alfalfa Congress Global Interaction for Alfalfa Innovation. Cordoba, Agentina, 11-14 noviembre, 2018, p. 178
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
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