Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination

Autores
Roqueiro, Gonzalo; Causin, Humberto Fabio; Ollé Resa, Carolina Patricia; Maroder, Horacio; Maldonado, Sara Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons.
EEA San Juan
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Causin, Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ollé Resa, Carolina Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Maroder, Horacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fuente
Physiologia Plantarum 149 (2) : 286-295. (October 2013)
Materia
Salix
Plántulas
Germinación
Cotiledones
Morfología Vegetal
Seedlings
Germination
Cotyledons
Plant Morphology
Sauce (salix)
Willow
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4768
Acceder
id INTADig_50001c4624340aaaf0961453e8076b72
oai_identifier_str oai:localhost:20.500.12123/4768
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germinationRoqueiro, GonzaloCausin, Humberto FabioOllé Resa, Carolina PatriciaMaroder, HoracioMaldonado, Sara BeatrizSalixPlántulasGerminaciónCotiledonesMorfología VegetalSeedlingsGerminationCotyledonsPlant MorphologySauce (salix)WillowIn willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons.EEA San JuanFil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Causin, Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Ollé Resa, Carolina Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Maroder, Horacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaWiley2019-03-28T12:57:31Z2019-03-28T12:57:31Z2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048http://hdl.handle.net/20.500.12123/47680031-93171399-3054https://doi.org/10.1111/ppl.12048Physiologia Plantarum 149 (2) : 286-295. (October 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:37Zoai:localhost:20.500.12123/4768instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:37.481INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
title Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
spellingShingle Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
Roqueiro, Gonzalo
Salix
Plántulas
Germinación
Cotiledones
Morfología Vegetal
Seedlings
Germination
Cotyledons
Plant Morphology
Sauce (salix)
Willow
title_short Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
title_full Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
title_fullStr Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
title_full_unstemmed Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
title_sort Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
dc.creator.none.fl_str_mv Roqueiro, Gonzalo
Causin, Humberto Fabio
Ollé Resa, Carolina Patricia
Maroder, Horacio
Maldonado, Sara Beatriz
author Roqueiro, Gonzalo
author_facet Roqueiro, Gonzalo
Causin, Humberto Fabio
Ollé Resa, Carolina Patricia
Maroder, Horacio
Maldonado, Sara Beatriz
author_role author
author2 Causin, Humberto Fabio
Ollé Resa, Carolina Patricia
Maroder, Horacio
Maldonado, Sara Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Salix
Plántulas
Germinación
Cotiledones
Morfología Vegetal
Seedlings
Germination
Cotyledons
Plant Morphology
Sauce (salix)
Willow
topic Salix
Plántulas
Germinación
Cotiledones
Morfología Vegetal
Seedlings
Germination
Cotyledons
Plant Morphology
Sauce (salix)
Willow
dc.description.none.fl_txt_mv In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons.
EEA San Juan
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Causin, Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ollé Resa, Carolina Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Maroder, Horacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2019-03-28T12:57:31Z
2019-03-28T12:57:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048
http://hdl.handle.net/20.500.12123/4768
0031-9317
1399-3054
https://doi.org/10.1111/ppl.12048
url https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12048
http://hdl.handle.net/20.500.12123/4768
https://doi.org/10.1111/ppl.12048
identifier_str_mv 0031-9317
1399-3054
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Physiologia Plantarum 149 (2) : 286-295. (October 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619132320874496
score 12.558318

Publicaciones similares