Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice

Autores
Lavari, Luisina; Burns, Patricia Graciela; Paez, Roxana Beatriz; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation.
EEA Rafaela
Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fuente
Journal of applied microbiology 123 (4) : 992-1002. (October 2017)
Materia
Lactobacillus
Probióticos
Lactosuero
Almidón
Ratón
Probiotics
Whey
Starch
Mice
Spray Drying
Secado por Pulverización
Lactobacillus rhamnosus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3291
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3291
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network_name_str INTA Digital (INTA)
spelling Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of miceLavari, LuisinaBurns, Patricia GracielaPaez, Roxana BeatrizReinheimer, Jorge AlbertoVinderola, Celso GabrielLactobacillusProbióticosLactosueroAlmidónRatónProbioticsWheyStarchMiceSpray DryingSecado por PulverizaciónLactobacillus rhamnosusAims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation.EEA RafaelaFil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; ArgentinaFil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina2018-09-04T15:58:40Z2018-09-04T15:58:40Z2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567http://hdl.handle.net/20.500.12123/32911364-50721365-2672https://doi.org/10.1111/jam.13567Journal of applied microbiology 123 (4) : 992-1002. (October 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:25Zoai:localhost:20.500.12123/3291instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:26.011INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
title Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
spellingShingle Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
Lavari, Luisina
Lactobacillus
Probióticos
Lactosuero
Almidón
Ratón
Probiotics
Whey
Starch
Mice
Spray Drying
Secado por Pulverización
Lactobacillus rhamnosus
title_short Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
title_full Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
title_fullStr Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
title_full_unstemmed Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
title_sort Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
dc.creator.none.fl_str_mv Lavari, Luisina
Burns, Patricia Graciela
Paez, Roxana Beatriz
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author Lavari, Luisina
author_facet Lavari, Luisina
Burns, Patricia Graciela
Paez, Roxana Beatriz
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author_role author
author2 Burns, Patricia Graciela
Paez, Roxana Beatriz
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus
Probióticos
Lactosuero
Almidón
Ratón
Probiotics
Whey
Starch
Mice
Spray Drying
Secado por Pulverización
Lactobacillus rhamnosus
topic Lactobacillus
Probióticos
Lactosuero
Almidón
Ratón
Probiotics
Whey
Starch
Mice
Spray Drying
Secado por Pulverización
Lactobacillus rhamnosus
dc.description.none.fl_txt_mv Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation.
EEA Rafaela
Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
description Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
2018-09-04T15:58:40Z
2018-09-04T15:58:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567
http://hdl.handle.net/20.500.12123/3291
1364-5072
1365-2672
https://doi.org/10.1111/jam.13567
url https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567
http://hdl.handle.net/20.500.12123/3291
https://doi.org/10.1111/jam.13567
identifier_str_mv 1364-5072
1365-2672
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of applied microbiology 123 (4) : 992-1002. (October 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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