Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease

Autores
Alessio, Florencia Ivette; Bongiorno, Vanina Aylén; von Backzo, Oscar Hernan; Marcone, Carmine; Conci, Luis Rogelio; Fernandez, Franco Daniel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study reports the draft genome of ‘Candidatus Phytoplasma pyri’ strain P1, isolated from Argentina. The genome assembly consisted of 17 contigs, with a total length of 575,431 bp, a GC content of 20.35%, and 125× coverage. A total of 537 genes were annotated, including those related to metabolism, genetic information processing, and signaling. Phylogenetic analysis placed ‘Ca. Phytoplasma pyri’ within the 16SrX group, supporting its classification as a distinct species from ‘Ca. Phytoplasma mali’ and ‘Ca. Phytoplasma prunorum’, with average nucleotide identity values ranging from 90.3 to 90.5%. In contrast, strain P1 shares 98.1% average nucleotide identity with the Chilean strain ‘Ca. Phytoplasma pyri’ UCh_1, supporting their classification as members of the same species. The investigation also identified 12 secreted proteins, including homologs of known effectors, as well as conserved proteins associated with virulence mechanisms. A homolog of the immunodominant membrane protein was also characterized, revealing conserved gene organization across related strains. These findings contribute to the understanding of ‘Ca. Phytoplasma pyri’ pathogenicity and provide valuable genomic data for future research.
Instituto de Patología Vegetal
Fil: Alessio, Florencia Ivette. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Alessio, Florencia Ivette. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: von Backzo, Oscar Hernan. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Marcone, Carmine. University of Salerno. Department of Pharmacy; Italia
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Fernandez, Franco Daniel.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
PhytoFrontiers 5 (3) : 272-542 (2025)
Materia
Genomes
Phytoplasmas
Pathogens
Genoma
Fitoplasmas
Organismos Patógenos
Apple Proliferation
Pear Decline
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24950
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24950
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline DiseaseAlessio, Florencia IvetteBongiorno, Vanina Aylénvon Backzo, Oscar HernanMarcone, CarmineConci, Luis RogelioFernandez, Franco DanielGenomesPhytoplasmasPathogensGenomaFitoplasmasOrganismos PatógenosApple ProliferationPear DeclineThis study reports the draft genome of ‘Candidatus Phytoplasma pyri’ strain P1, isolated from Argentina. The genome assembly consisted of 17 contigs, with a total length of 575,431 bp, a GC content of 20.35%, and 125× coverage. A total of 537 genes were annotated, including those related to metabolism, genetic information processing, and signaling. Phylogenetic analysis placed ‘Ca. Phytoplasma pyri’ within the 16SrX group, supporting its classification as a distinct species from ‘Ca. Phytoplasma mali’ and ‘Ca. Phytoplasma prunorum’, with average nucleotide identity values ranging from 90.3 to 90.5%. In contrast, strain P1 shares 98.1% average nucleotide identity with the Chilean strain ‘Ca. Phytoplasma pyri’ UCh_1, supporting their classification as members of the same species. The investigation also identified 12 secreted proteins, including homologs of known effectors, as well as conserved proteins associated with virulence mechanisms. A homolog of the immunodominant membrane protein was also characterized, revealing conserved gene organization across related strains. These findings contribute to the understanding of ‘Ca. Phytoplasma pyri’ pathogenicity and provide valuable genomic data for future research.Instituto de Patología VegetalFil: Alessio, Florencia Ivette. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Alessio, Florencia Ivette. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: von Backzo, Oscar Hernan. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Marcone, Carmine. University of Salerno. Department of Pharmacy; ItaliaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Fernandez, Franco Daniel.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaThe American Phytopathological Society2026-01-08T14:21:56Z2026-01-08T14:21:56Z2025-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24950https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-04-25-0038-A2690-5442https://doi.org/10.1094/PHYTOFR-04-25-0038-APhytoFrontiers 5 (3) : 272-542 (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedadesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:40Zoai:localhost:20.500.12123/24950instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:40.589INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
title Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
spellingShingle Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
Alessio, Florencia Ivette
Genomes
Phytoplasmas
Pathogens
Genoma
Fitoplasmas
Organismos Patógenos
Apple Proliferation
Pear Decline
title_short Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
title_full Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
title_fullStr Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
title_full_unstemmed Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
title_sort Draft Genome Sequence of ‘Candidatus Phytoplasma pyri’ Strain P1, the Causal Agent of Pear Decline Disease
dc.creator.none.fl_str_mv Alessio, Florencia Ivette
Bongiorno, Vanina Aylén
von Backzo, Oscar Hernan
Marcone, Carmine
Conci, Luis Rogelio
Fernandez, Franco Daniel
author Alessio, Florencia Ivette
author_facet Alessio, Florencia Ivette
Bongiorno, Vanina Aylén
von Backzo, Oscar Hernan
Marcone, Carmine
Conci, Luis Rogelio
Fernandez, Franco Daniel
author_role author
author2 Bongiorno, Vanina Aylén
von Backzo, Oscar Hernan
Marcone, Carmine
Conci, Luis Rogelio
Fernandez, Franco Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Genomes
Phytoplasmas
Pathogens
Genoma
Fitoplasmas
Organismos Patógenos
Apple Proliferation
Pear Decline
topic Genomes
Phytoplasmas
Pathogens
Genoma
Fitoplasmas
Organismos Patógenos
Apple Proliferation
Pear Decline
dc.description.none.fl_txt_mv This study reports the draft genome of ‘Candidatus Phytoplasma pyri’ strain P1, isolated from Argentina. The genome assembly consisted of 17 contigs, with a total length of 575,431 bp, a GC content of 20.35%, and 125× coverage. A total of 537 genes were annotated, including those related to metabolism, genetic information processing, and signaling. Phylogenetic analysis placed ‘Ca. Phytoplasma pyri’ within the 16SrX group, supporting its classification as a distinct species from ‘Ca. Phytoplasma mali’ and ‘Ca. Phytoplasma prunorum’, with average nucleotide identity values ranging from 90.3 to 90.5%. In contrast, strain P1 shares 98.1% average nucleotide identity with the Chilean strain ‘Ca. Phytoplasma pyri’ UCh_1, supporting their classification as members of the same species. The investigation also identified 12 secreted proteins, including homologs of known effectors, as well as conserved proteins associated with virulence mechanisms. A homolog of the immunodominant membrane protein was also characterized, revealing conserved gene organization across related strains. These findings contribute to the understanding of ‘Ca. Phytoplasma pyri’ pathogenicity and provide valuable genomic data for future research.
Instituto de Patología Vegetal
Fil: Alessio, Florencia Ivette. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Alessio, Florencia Ivette. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: von Backzo, Oscar Hernan. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Marcone, Carmine. University of Salerno. Department of Pharmacy; Italia
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Fernandez, Franco Daniel.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description This study reports the draft genome of ‘Candidatus Phytoplasma pyri’ strain P1, isolated from Argentina. The genome assembly consisted of 17 contigs, with a total length of 575,431 bp, a GC content of 20.35%, and 125× coverage. A total of 537 genes were annotated, including those related to metabolism, genetic information processing, and signaling. Phylogenetic analysis placed ‘Ca. Phytoplasma pyri’ within the 16SrX group, supporting its classification as a distinct species from ‘Ca. Phytoplasma mali’ and ‘Ca. Phytoplasma prunorum’, with average nucleotide identity values ranging from 90.3 to 90.5%. In contrast, strain P1 shares 98.1% average nucleotide identity with the Chilean strain ‘Ca. Phytoplasma pyri’ UCh_1, supporting their classification as members of the same species. The investigation also identified 12 secreted proteins, including homologs of known effectors, as well as conserved proteins associated with virulence mechanisms. A homolog of the immunodominant membrane protein was also characterized, revealing conserved gene organization across related strains. These findings contribute to the understanding of ‘Ca. Phytoplasma pyri’ pathogenicity and provide valuable genomic data for future research.
publishDate 2025
dc.date.none.fl_str_mv 2025-08-21
2026-01-08T14:21:56Z
2026-01-08T14:21:56Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24950
https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-04-25-0038-A
2690-5442
https://doi.org/10.1094/PHYTOFR-04-25-0038-A
url http://hdl.handle.net/20.500.12123/24950
https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-04-25-0038-A
https://doi.org/10.1094/PHYTOFR-04-25-0038-A
identifier_str_mv 2690-5442
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
info:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedades
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The American Phytopathological Society
publisher.none.fl_str_mv The American Phytopathological Society
dc.source.none.fl_str_mv PhytoFrontiers 5 (3) : 272-542 (2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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