Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste

Autores
Heredia, Belén; Diaz Recio, Pablo; Young, Brian Jonathan; Pacheco, Pablo; Barcelo Zungre, Franco; Hruby, Silvina Anabela; Acevedo, Maria Andreína; Mercado, María Inés; Roqueiro, Gonzalo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con­ centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi­ cochemical characteristics for plant survival at highest contamination levels
EEA San Juan
Fil: Heredia, Belén. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Heredia, Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Diaz Recio, Pablo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pacheco, Pablo. Universidad Nacional de San Luis (UNSL). Instituto de Química San Luis; Argentina
Fil: Pacheco, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química San Luis; Argentina
Fil: Barcelo Zungre, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Barcelo Zungre, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina
Fil: Hruby, Silvina Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Hruby, Silvina Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina
Fil: Acevedo, Maria Andreína. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina
Fil: Mercado, María Inés. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina
Fil: Roqueiro, Gonzalo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fuente
Ecotoxicology and Environmental Safety 290 (1) : 117750 (Enero 2025)
Materia
Estrés Oxidativo
Fitodecontaminación
Polución del suelo
Fitotoxicidad
Plántula
Organismos Nativos
Oxidative Stress
Phytoremediation
Soil Pollution
Phytotoxicity
Seedlings
Native Organisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21124
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21124
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining wasteHeredia, BelénDiaz Recio, PabloYoung, Brian JonathanPacheco, PabloBarcelo Zungre, FrancoHruby, Silvina AnabelaAcevedo, Maria AndreínaMercado, María InésRoqueiro, GonzaloEstrés OxidativoFitodecontaminaciónPolución del sueloFitotoxicidadPlántulaOrganismos NativosOxidative StressPhytoremediationSoil PollutionPhytotoxicitySeedlingsNative OrganismsSeeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con­ centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi­ cochemical characteristics for plant survival at highest contamination levelsEEA San JuanFil: Heredia, Belén. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; ArgentinaFil: Heredia, Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Diaz Recio, Pablo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; ArgentinaFil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Pacheco, Pablo. Universidad Nacional de San Luis (UNSL). Instituto de Química San Luis; ArgentinaFil: Pacheco, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química San Luis; ArgentinaFil: Barcelo Zungre, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Barcelo Zungre, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; ArgentinaFil: Hruby, Silvina Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaFil: Hruby, Silvina Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; ArgentinaFil: Acevedo, Maria Andreína. Fundación Miguel Lillo. Instituto de Morfología Vegetal; ArgentinaFil: Mercado, María Inés. Fundación Miguel Lillo. Instituto de Morfología Vegetal; ArgentinaFil: Roqueiro, Gonzalo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; ArgentinaFil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; ArgentinaElsevier2025-02-04T13:30:13Z2025-02-04T13:30:13Z2025-01-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21124https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub0147-6513https://doi.org/10.1016/j.ecoenv.2025.117750Ecotoxicology and Environmental Safety 290 (1) : 117750 (Enero 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I096, Estrategias de restauración, valoración y conservación de la biodiversidad y servicios ecosistemicosinfo:eu-repograntAgreement/INTA/2023-PE-L02-I003, Gestión sostenible de los recursos naturales para la producción y la población rural en ambientes áridos, con énfasis en el recurso hídricoinfo:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregadoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:04Zoai:localhost:20.500.12123/21124instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:06.817INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
title Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
spellingShingle Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
Heredia, Belén
Estrés Oxidativo
Fitodecontaminación
Polución del suelo
Fitotoxicidad
Plántula
Organismos Nativos
Oxidative Stress
Phytoremediation
Soil Pollution
Phytotoxicity
Seedlings
Native Organisms
title_short Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
title_full Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
title_fullStr Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
title_full_unstemmed Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
title_sort Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
dc.creator.none.fl_str_mv Heredia, Belén
Diaz Recio, Pablo
Young, Brian Jonathan
Pacheco, Pablo
Barcelo Zungre, Franco
Hruby, Silvina Anabela
Acevedo, Maria Andreína
Mercado, María Inés
Roqueiro, Gonzalo
author Heredia, Belén
author_facet Heredia, Belén
Diaz Recio, Pablo
Young, Brian Jonathan
Pacheco, Pablo
Barcelo Zungre, Franco
Hruby, Silvina Anabela
Acevedo, Maria Andreína
Mercado, María Inés
Roqueiro, Gonzalo
author_role author
author2 Diaz Recio, Pablo
Young, Brian Jonathan
Pacheco, Pablo
Barcelo Zungre, Franco
Hruby, Silvina Anabela
Acevedo, Maria Andreína
Mercado, María Inés
Roqueiro, Gonzalo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrés Oxidativo
Fitodecontaminación
Polución del suelo
Fitotoxicidad
Plántula
Organismos Nativos
Oxidative Stress
Phytoremediation
Soil Pollution
Phytotoxicity
Seedlings
Native Organisms
topic Estrés Oxidativo
Fitodecontaminación
Polución del suelo
Fitotoxicidad
Plántula
Organismos Nativos
Oxidative Stress
Phytoremediation
Soil Pollution
Phytotoxicity
Seedlings
Native Organisms
dc.description.none.fl_txt_mv Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con­ centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi­ cochemical characteristics for plant survival at highest contamination levels
EEA San Juan
Fil: Heredia, Belén. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Heredia, Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Diaz Recio, Pablo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pacheco, Pablo. Universidad Nacional de San Luis (UNSL). Instituto de Química San Luis; Argentina
Fil: Pacheco, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química San Luis; Argentina
Fil: Barcelo Zungre, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Barcelo Zungre, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina
Fil: Hruby, Silvina Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
Fil: Hruby, Silvina Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina
Fil: Acevedo, Maria Andreína. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina
Fil: Mercado, María Inés. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina
Fil: Roqueiro, Gonzalo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina
description Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con­ centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi­ cochemical characteristics for plant survival at highest contamination levels
publishDate 2025
dc.date.none.fl_str_mv 2025-02-04T13:30:13Z
2025-02-04T13:30:13Z
2025-01-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21124
https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub
0147-6513
https://doi.org/10.1016/j.ecoenv.2025.117750
url http://hdl.handle.net/20.500.12123/21124
https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub
https://doi.org/10.1016/j.ecoenv.2025.117750
identifier_str_mv 0147-6513
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I096, Estrategias de restauración, valoración y conservación de la biodiversidad y servicios ecosistemicos
info:eu-repograntAgreement/INTA/2023-PE-L02-I003, Gestión sostenible de los recursos naturales para la producción y la población rural en ambientes áridos, con énfasis en el recurso hídrico
info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ecotoxicology and Environmental Safety 290 (1) : 117750 (Enero 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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