Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb

Autores
Ben Guerrero, Emiliano; Lasa, Ana V.; Aguilera, Pablo Nicolás; Fernández-González, Antonio J.; Martinez, Maria Carolina; Mercado Blanco, Jesús; Fernández-López, Manuel; Paniego, Norma Beatriz
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop.
Instituto de Biotecnología
Fil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ben Guerrero, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ben Guerrero, Emiliano. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Lasa, Ana V. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Aguilera, Pablo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Aguilera, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández-González, Antonio J. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Fernández-López, Manuel. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Applied Soil Ecology 206 : 105915 (February 2025)
Materia
Verticillium Wilt
Disease Resistance
Genotypes
Metabarcoding
Ribosomal RNA
Microbiomes
Rhizosphere
Sunflowers
Marchitez por Verticillium
Resistencia a la Enfermedad
Genotipos
Metabarcodificación
Arn Ribosomial
Microbioma
Rizosfera
Girasol
Helianthus annuus
Lysobacter
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21478
Acceder
id INTADig_4b0945e8ce38fe01a42f351ad114b30f
oai_identifier_str oai:localhost:20.500.12123/21478
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae KlebBen Guerrero, EmilianoLasa, Ana V.Aguilera, Pablo NicolásFernández-González, Antonio J.Martinez, Maria CarolinaMercado Blanco, JesúsFernández-López, ManuelPaniego, Norma BeatrizVerticillium WiltDisease ResistanceGenotypesMetabarcodingRibosomal RNAMicrobiomesRhizosphereSunflowersMarchitez por VerticilliumResistencia a la EnfermedadGenotiposMetabarcodificaciónArn RibosomialMicrobiomaRizosferaGirasolHelianthus annuusLysobacterPlants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop.Instituto de BiotecnologíaFil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ben Guerrero, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ben Guerrero, Emiliano. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; EspañaFil: Lasa, Ana V. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; EspañaFil: Aguilera, Pablo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Aguilera, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández-González, Antonio J. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; EspañaFil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; EspañaFil: Fernández-López, Manuel. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; EspañaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2025-02-26T14:59:50Z2025-02-26T14:59:50Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21478https://www.sciencedirect.com/science/article/abs/pii/S09291393250005380929-1393https://doi.org/10.1016/j.apsoil.2025.105915Applied Soil Ecology 206 : 105915 (February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:10Zoai:localhost:20.500.12123/21478instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:11.308INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
title Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
spellingShingle Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
Ben Guerrero, Emiliano
Verticillium Wilt
Disease Resistance
Genotypes
Metabarcoding
Ribosomal RNA
Microbiomes
Rhizosphere
Sunflowers
Marchitez por Verticillium
Resistencia a la Enfermedad
Genotipos
Metabarcodificación
Arn Ribosomial
Microbioma
Rizosfera
Girasol
Helianthus annuus
Lysobacter
title_short Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
title_full Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
title_fullStr Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
title_full_unstemmed Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
title_sort Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
dc.creator.none.fl_str_mv Ben Guerrero, Emiliano
Lasa, Ana V.
Aguilera, Pablo Nicolás
Fernández-González, Antonio J.
Martinez, Maria Carolina
Mercado Blanco, Jesús
Fernández-López, Manuel
Paniego, Norma Beatriz
author Ben Guerrero, Emiliano
author_facet Ben Guerrero, Emiliano
Lasa, Ana V.
Aguilera, Pablo Nicolás
Fernández-González, Antonio J.
Martinez, Maria Carolina
Mercado Blanco, Jesús
Fernández-López, Manuel
Paniego, Norma Beatriz
author_role author
author2 Lasa, Ana V.
Aguilera, Pablo Nicolás
Fernández-González, Antonio J.
Martinez, Maria Carolina
Mercado Blanco, Jesús
Fernández-López, Manuel
Paniego, Norma Beatriz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Verticillium Wilt
Disease Resistance
Genotypes
Metabarcoding
Ribosomal RNA
Microbiomes
Rhizosphere
Sunflowers
Marchitez por Verticillium
Resistencia a la Enfermedad
Genotipos
Metabarcodificación
Arn Ribosomial
Microbioma
Rizosfera
Girasol
Helianthus annuus
Lysobacter
topic Verticillium Wilt
Disease Resistance
Genotypes
Metabarcoding
Ribosomal RNA
Microbiomes
Rhizosphere
Sunflowers
Marchitez por Verticillium
Resistencia a la Enfermedad
Genotipos
Metabarcodificación
Arn Ribosomial
Microbioma
Rizosfera
Girasol
Helianthus annuus
Lysobacter
dc.description.none.fl_txt_mv Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop.
Instituto de Biotecnología
Fil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ben Guerrero, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ben Guerrero, Emiliano. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Lasa, Ana V. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Aguilera, Pablo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Aguilera, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández-González, Antonio J. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Fernández-López, Manuel. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-26T14:59:50Z
2025-02-26T14:59:50Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21478
https://www.sciencedirect.com/science/article/abs/pii/S0929139325000538
0929-1393
https://doi.org/10.1016/j.apsoil.2025.105915
url http://hdl.handle.net/20.500.12123/21478
https://www.sciencedirect.com/science/article/abs/pii/S0929139325000538
https://doi.org/10.1016/j.apsoil.2025.105915
identifier_str_mv 0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 206 : 105915 (February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143586600484864
score 12.712165

Publicaciones similares