Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation

Autores
Gimenez, Magali Diana; Yañez Santos, Anahi Mara; Paz, Rosalia Cristina; Quiroga, Mariana Paola; Marfil, Carlos Federico; Conci, Vilma Cecilia; Garcia Lampasona, Sandra Claudia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.
EEA Mendoza
Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Yañez Santos, Anahi Mara. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina
Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina
Fil: Marfil, Carlos Fedrico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fuente
Plant Cell Reports 35 (1) : 129–141 (January 2016)
Materia
Genética
Ajo
Variación Somaclonal
Experimentación In Vitro
Allium Sativum
Cultivo de Meristemas
Genetics
Garlic
Somaclonal Variation
In Vitro Experimentation
Meristem Culture
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagationGimenez, Magali DianaYañez Santos, Anahi MaraPaz, Rosalia CristinaQuiroga, Mariana PaolaMarfil, Carlos FedericoConci, Vilma CeciliaGarcia Lampasona, Sandra ClaudiaGenéticaAjoVariación SomaclonalExperimentación In VitroAllium SativumCultivo de MeristemasGeneticsGarlicSomaclonal VariationIn Vitro ExperimentationMeristem CultureGarlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.EEA MendozaFil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; ArgentinaFil: Yañez Santos, Anahi Mara. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; ArgentinaFil: Paz, Rosalia Cristina. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; ArgentinaFil: Marfil, Carlos Fedrico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; ArgentinaFil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina2017-11-01T12:16:47Z2017-11-01T12:16:47Z2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1640https://link.springer.com/article/10.1007%2Fs00299-015-1874-x0721-7714 (Print)1432-203X (Online)https://doi.org/10.1007/s00299-015-1874-xPlant Cell Reports 35 (1) : 129–141 (January 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:06Zoai:localhost:20.500.12123/1640instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:07.467INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
title Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
spellingShingle Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
Gimenez, Magali Diana
Genética
Ajo
Variación Somaclonal
Experimentación In Vitro
Allium Sativum
Cultivo de Meristemas
Genetics
Garlic
Somaclonal Variation
In Vitro Experimentation
Meristem Culture
title_short Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
title_full Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
title_fullStr Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
title_full_unstemmed Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
title_sort Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
dc.creator.none.fl_str_mv Gimenez, Magali Diana
Yañez Santos, Anahi Mara
Paz, Rosalia Cristina
Quiroga, Mariana Paola
Marfil, Carlos Federico
Conci, Vilma Cecilia
Garcia Lampasona, Sandra Claudia
author Gimenez, Magali Diana
author_facet Gimenez, Magali Diana
Yañez Santos, Anahi Mara
Paz, Rosalia Cristina
Quiroga, Mariana Paola
Marfil, Carlos Federico
Conci, Vilma Cecilia
Garcia Lampasona, Sandra Claudia
author_role author
author2 Yañez Santos, Anahi Mara
Paz, Rosalia Cristina
Quiroga, Mariana Paola
Marfil, Carlos Federico
Conci, Vilma Cecilia
Garcia Lampasona, Sandra Claudia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Ajo
Variación Somaclonal
Experimentación In Vitro
Allium Sativum
Cultivo de Meristemas
Genetics
Garlic
Somaclonal Variation
In Vitro Experimentation
Meristem Culture
topic Genética
Ajo
Variación Somaclonal
Experimentación In Vitro
Allium Sativum
Cultivo de Meristemas
Genetics
Garlic
Somaclonal Variation
In Vitro Experimentation
Meristem Culture
dc.description.none.fl_txt_mv Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.
EEA Mendoza
Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Yañez Santos, Anahi Mara. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina
Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina
Fil: Marfil, Carlos Fedrico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
description Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
2017-11-01T12:16:47Z
2017-11-01T12:16:47Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1640
https://link.springer.com/article/10.1007%2Fs00299-015-1874-x
0721-7714 (Print)
1432-203X (Online)
https://doi.org/10.1007/s00299-015-1874-x
url http://hdl.handle.net/20.500.12123/1640
https://link.springer.com/article/10.1007%2Fs00299-015-1874-x
https://doi.org/10.1007/s00299-015-1874-x
identifier_str_mv 0721-7714 (Print)
1432-203X (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Cell Reports 35 (1) : 129–141 (January 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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