Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome

Autores
Marquez, Nathalie; Giachero, María Lorena; Gallou, Adrien; Debat, Humberto Julio; Declerck, Stéphane; Ducasse, Daniel Adrian
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme.
Instituto de Patología Vegetal
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology. Mycology. Louvain-la-Neuve; Bélgica
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Plant Pathology 68 (3) : 470- 480 (2019)
Materia
Mycorrhizae
Fusarium
Soja
Soybeans
Fusarium Virguliforme
Mycorrhiza
Sudden Death Syndrome
Transcriptome
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8439
Acceder
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spelling Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndromeMarquez, NathalieGiachero, María LorenaGallou, AdrienDebat, Humberto JulioDeclerck, StéphaneDucasse, Daniel AdrianMycorrhizaeFusariumSojaSoybeansFusarium VirguliformeMycorrhizaSudden Death SyndromeTranscriptomeSoilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme.Instituto de Patología VegetalFil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México.Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology. Mycology. Louvain-la-Neuve; BélgicaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina2020-12-16T16:16:22Z2020-12-16T16:16:22Z2018-11-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8439https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.129640032-08621365-3059 (online)https://doi.org/10.1111/ppa.12964Plant Pathology 68 (3) : 470- 480 (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:58Zoai:localhost:20.500.12123/8439instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:58.51INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
title Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
spellingShingle Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
Marquez, Nathalie
Mycorrhizae
Fusarium
Soja
Soybeans
Fusarium Virguliforme
Mycorrhiza
Sudden Death Syndrome
Transcriptome
title_short Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
title_full Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
title_fullStr Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
title_full_unstemmed Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
title_sort Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
dc.creator.none.fl_str_mv Marquez, Nathalie
Giachero, María Lorena
Gallou, Adrien
Debat, Humberto Julio
Declerck, Stéphane
Ducasse, Daniel Adrian
author Marquez, Nathalie
author_facet Marquez, Nathalie
Giachero, María Lorena
Gallou, Adrien
Debat, Humberto Julio
Declerck, Stéphane
Ducasse, Daniel Adrian
author_role author
author2 Giachero, María Lorena
Gallou, Adrien
Debat, Humberto Julio
Declerck, Stéphane
Ducasse, Daniel Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mycorrhizae
Fusarium
Soja
Soybeans
Fusarium Virguliforme
Mycorrhiza
Sudden Death Syndrome
Transcriptome
topic Mycorrhizae
Fusarium
Soja
Soybeans
Fusarium Virguliforme
Mycorrhiza
Sudden Death Syndrome
Transcriptome
dc.description.none.fl_txt_mv Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme.
Instituto de Patología Vegetal
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology. Mycology. Louvain-la-Neuve; Bélgica
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-21
2020-12-16T16:16:22Z
2020-12-16T16:16:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8439
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12964
0032-0862
1365-3059 (online)
https://doi.org/10.1111/ppa.12964
url http://hdl.handle.net/20.500.12123/8439
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12964
https://doi.org/10.1111/ppa.12964
identifier_str_mv 0032-0862
1365-3059 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Pathology 68 (3) : 470- 480 (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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