Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication

Autores
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Soto, Gabriela Cinthia; Jozefkowicz, Cintia; Ayub, Nicolás Daniel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production.
Instituto de Genética
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Microbial Ecology 76 (2): 299–302 (August 2018)
Materia
Óxido Nitroso
Medicago sativa
Fijación del Nitrógeno
Muestreo cluster
Genes
Rhizobium
Nitrous Oxide
Nitrogen Fixation
Cluster Sampling
Inoculantes comerciales
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3399
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oai_identifier_str oai:localhost:20.500.12123/3399
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network_name_str INTA Digital (INTA)
spelling Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domesticationBrambilla, Silvina MaricelFrare, Romina AlejandraSoto, Gabriela CinthiaJozefkowicz, CintiaAyub, Nicolás DanielÓxido NitrosoMedicago sativaFijación del NitrógenoMuestreo clusterGenesRhizobiumNitrous OxideNitrogen FixationCluster SamplingInoculantes comercialesAs other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production.Instituto de GenéticaFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2018-09-19T17:04:53Z2018-09-19T17:04:53Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00248-018-1145-9http://hdl.handle.net/20.500.12123/33990095-36281432-184Xhttps://doi.org/10.1007/s00248-018-1145-9Microbial Ecology 76 (2): 299–302 (August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-27T08:37:56Zoai:localhost:20.500.12123/3399instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:37:57.032INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
title Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
spellingShingle Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
Brambilla, Silvina Maricel
Óxido Nitroso
Medicago sativa
Fijación del Nitrógeno
Muestreo cluster
Genes
Rhizobium
Nitrous Oxide
Nitrogen Fixation
Cluster Sampling
Inoculantes comerciales
title_short Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
title_full Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
title_fullStr Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
title_full_unstemmed Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
title_sort Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
dc.creator.none.fl_str_mv Brambilla, Silvina Maricel
Frare, Romina Alejandra
Soto, Gabriela Cinthia
Jozefkowicz, Cintia
Ayub, Nicolás Daniel
author Brambilla, Silvina Maricel
author_facet Brambilla, Silvina Maricel
Frare, Romina Alejandra
Soto, Gabriela Cinthia
Jozefkowicz, Cintia
Ayub, Nicolás Daniel
author_role author
author2 Frare, Romina Alejandra
Soto, Gabriela Cinthia
Jozefkowicz, Cintia
Ayub, Nicolás Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Óxido Nitroso
Medicago sativa
Fijación del Nitrógeno
Muestreo cluster
Genes
Rhizobium
Nitrous Oxide
Nitrogen Fixation
Cluster Sampling
Inoculantes comerciales
topic Óxido Nitroso
Medicago sativa
Fijación del Nitrógeno
Muestreo cluster
Genes
Rhizobium
Nitrous Oxide
Nitrogen Fixation
Cluster Sampling
Inoculantes comerciales
dc.description.none.fl_txt_mv As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production.
Instituto de Genética
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-19T17:04:53Z
2018-09-19T17:04:53Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00248-018-1145-9
http://hdl.handle.net/20.500.12123/3399
0095-3628
1432-184X
https://doi.org/10.1007/s00248-018-1145-9
url https://link.springer.com/article/10.1007/s00248-018-1145-9
http://hdl.handle.net/20.500.12123/3399
https://doi.org/10.1007/s00248-018-1145-9
identifier_str_mv 0095-3628
1432-184X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Microbial Ecology 76 (2): 299–302 (August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.275514

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