Role of calcium in the defense response induced by brassinosteroids in strawberry plants

Autores
Furio, Ramiro Nicolás; Martinez Zamora, Martin Gustavo; Salazar, Sergio Miguel; Coll, Yamilet; Perato, Silvia Marisa; Martos, Gustavo Gabriel; Díaz Ricci, Juan Carlos
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants.
EEA Famaillá
Fil: Furio, R.N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Furio, R.N. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Coll, Yamilet. Universidad La Habana. Centro de Estudio de Productos Naturales; Cuba
Fil: Perato, Silvia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Perato, Silvia Marisa. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martos, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martos, Gustavo Gabriel. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina.
Fuente
Scientia Horticulturae 261 : 109010 (February 2020)
Materia
Fresa
Fragaria ananassa
Brasinoesteroides
Calcio
Calmodulina
Strawberries
Brassinosteroids
Calcium
Calmodulin
Immune Response
Respuesta Inmunológica
Frutilla
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10410
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10410
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Role of calcium in the defense response induced by brassinosteroids in strawberry plantsFurio, Ramiro NicolásMartinez Zamora, Martin GustavoSalazar, Sergio MiguelColl, YamiletPerato, Silvia MarisaMartos, Gustavo GabrielDíaz Ricci, Juan CarlosFresaFragaria ananassaBrasinoesteroidesCalcioCalmodulinaStrawberriesBrassinosteroidsCalciumCalmodulinImmune ResponseRespuesta InmunológicaFrutillaBrassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants.EEA FamailláFil: Furio, R.N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Furio, R.N. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Coll, Yamilet. Universidad La Habana. Centro de Estudio de Productos Naturales; CubaFil: Perato, Silvia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Perato, Silvia Marisa. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martos, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martos, Gustavo Gabriel. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina.Elsevier2021-10-04T12:41:04Z2021-10-04T12:41:04Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10410https://www.sciencedirect.com/science/article/pii/S03044238193089690304-4238https://doi.org/10.1016/j.scienta.2019.109010Scientia Horticulturae 261 : 109010 (February 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas.info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán.info:eu-repo/semantics/restrictedAccess2025-10-16T09:30:14Zoai:localhost:20.500.12123/10410instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:15.227INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Role of calcium in the defense response induced by brassinosteroids in strawberry plants
title Role of calcium in the defense response induced by brassinosteroids in strawberry plants
spellingShingle Role of calcium in the defense response induced by brassinosteroids in strawberry plants
Furio, Ramiro Nicolás
Fresa
Fragaria ananassa
Brasinoesteroides
Calcio
Calmodulina
Strawberries
Brassinosteroids
Calcium
Calmodulin
Immune Response
Respuesta Inmunológica
Frutilla
title_short Role of calcium in the defense response induced by brassinosteroids in strawberry plants
title_full Role of calcium in the defense response induced by brassinosteroids in strawberry plants
title_fullStr Role of calcium in the defense response induced by brassinosteroids in strawberry plants
title_full_unstemmed Role of calcium in the defense response induced by brassinosteroids in strawberry plants
title_sort Role of calcium in the defense response induced by brassinosteroids in strawberry plants
dc.creator.none.fl_str_mv Furio, Ramiro Nicolás
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
Coll, Yamilet
Perato, Silvia Marisa
Martos, Gustavo Gabriel
Díaz Ricci, Juan Carlos
author Furio, Ramiro Nicolás
author_facet Furio, Ramiro Nicolás
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
Coll, Yamilet
Perato, Silvia Marisa
Martos, Gustavo Gabriel
Díaz Ricci, Juan Carlos
author_role author
author2 Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
Coll, Yamilet
Perato, Silvia Marisa
Martos, Gustavo Gabriel
Díaz Ricci, Juan Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fresa
Fragaria ananassa
Brasinoesteroides
Calcio
Calmodulina
Strawberries
Brassinosteroids
Calcium
Calmodulin
Immune Response
Respuesta Inmunológica
Frutilla
topic Fresa
Fragaria ananassa
Brasinoesteroides
Calcio
Calmodulina
Strawberries
Brassinosteroids
Calcium
Calmodulin
Immune Response
Respuesta Inmunológica
Frutilla
dc.description.none.fl_txt_mv Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants.
EEA Famaillá
Fil: Furio, R.N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Furio, R.N. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Coll, Yamilet. Universidad La Habana. Centro de Estudio de Productos Naturales; Cuba
Fil: Perato, Silvia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Perato, Silvia Marisa. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martos, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martos, Gustavo Gabriel. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina.
description Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2021-10-04T12:41:04Z
2021-10-04T12:41:04Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10410
https://www.sciencedirect.com/science/article/pii/S0304423819308969
0304-4238
https://doi.org/10.1016/j.scienta.2019.109010
url http://hdl.handle.net/20.500.12123/10410
https://www.sciencedirect.com/science/article/pii/S0304423819308969
https://doi.org/10.1016/j.scienta.2019.109010
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas.
info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Scientia Horticulturae 261 : 109010 (February 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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