DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin

Autores
Pautasso, Nestor Abel; Poletta, Gisela Laura; Paravani, Enrique Valentín; Sasal, Maria Carolina; Simoniello, María Fernanda
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receive rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72 h after a probed natural runoff event towards the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT) and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.
EEA Paraná
Fil: Pautasso, Néstor. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Poletta, Gisela L. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; Argentina
Fil: Poletta, Gisela L. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Paravani, Enrique Valentín. Universidad de Entre Ríos. Facultad de Ingeniería. Cátedra de Química General e Inorgánica. Laboratorio de Química Ambiental; Argentina
Fil: Sasal, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Simoniello, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; Argentina.
Fuente
Environmental and Molecular Mutagenesis : 1-34 (First published: 31 January 2023)
Materia
Cnesterodon
Plaguicidas
Branquias
Escorrentia
Estrés Oxidativo
Cuencas
Pesticides
Gills
Runoff
Oxidative Stress
Basins
Cnesterodon decemmaculatus
Desagüe
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14021

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oai_identifier_str oai:localhost:20.500.12123/14021
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network_name_str INTA Digital (INTA)
spelling DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basinPautasso, Nestor AbelPoletta, Gisela LauraParavani, Enrique ValentínSasal, Maria CarolinaSimoniello, María FernandaCnesterodonPlaguicidasBranquiasEscorrentiaEstrés OxidativoCuencasPesticidesGillsRunoffOxidative StressBasinsCnesterodon decemmaculatusDesagüeIn our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receive rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72 h after a probed natural runoff event towards the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT) and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.EEA ParanáFil: Pautasso, Néstor. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; ArgentinaFil: Poletta, Gisela L. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; ArgentinaFil: Poletta, Gisela L. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Paravani, Enrique Valentín. Universidad de Entre Ríos. Facultad de Ingeniería. Cátedra de Química General e Inorgánica. Laboratorio de Química Ambiental; ArgentinaFil: Sasal, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; ArgentinaFil: Simoniello, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; Argentina.Wileyinfo:eu-repo/date/embargoEnd/2024-01-172023-02-17T15:21:29Z2023-02-17T15:21:29Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14021https://onlinelibrary.wiley.com/doi/10.1002/em.225290893-66921098-2280https://doi.org/10.1002/em.22529Environmental and Molecular Mutagenesis : 1-34 (First published: 31 January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNNAT-1128043/AR./Los agroquímicos como fuente de contaminación difusa en agroecosistemas.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:54Zoai:localhost:20.500.12123/14021instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:54.843INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
title DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
spellingShingle DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
Pautasso, Nestor Abel
Cnesterodon
Plaguicidas
Branquias
Escorrentia
Estrés Oxidativo
Cuencas
Pesticides
Gills
Runoff
Oxidative Stress
Basins
Cnesterodon decemmaculatus
Desagüe
title_short DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
title_full DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
title_fullStr DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
title_full_unstemmed DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
title_sort DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin
dc.creator.none.fl_str_mv Pautasso, Nestor Abel
Poletta, Gisela Laura
Paravani, Enrique Valentín
Sasal, Maria Carolina
Simoniello, María Fernanda
author Pautasso, Nestor Abel
author_facet Pautasso, Nestor Abel
Poletta, Gisela Laura
Paravani, Enrique Valentín
Sasal, Maria Carolina
Simoniello, María Fernanda
author_role author
author2 Poletta, Gisela Laura
Paravani, Enrique Valentín
Sasal, Maria Carolina
Simoniello, María Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cnesterodon
Plaguicidas
Branquias
Escorrentia
Estrés Oxidativo
Cuencas
Pesticides
Gills
Runoff
Oxidative Stress
Basins
Cnesterodon decemmaculatus
Desagüe
topic Cnesterodon
Plaguicidas
Branquias
Escorrentia
Estrés Oxidativo
Cuencas
Pesticides
Gills
Runoff
Oxidative Stress
Basins
Cnesterodon decemmaculatus
Desagüe
dc.description.none.fl_txt_mv In our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receive rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72 h after a probed natural runoff event towards the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT) and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.
EEA Paraná
Fil: Pautasso, Néstor. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Poletta, Gisela L. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; Argentina
Fil: Poletta, Gisela L. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Paravani, Enrique Valentín. Universidad de Entre Ríos. Facultad de Ingeniería. Cátedra de Química General e Inorgánica. Laboratorio de Química Ambiental; Argentina
Fil: Sasal, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Simoniello, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología, Farmacología y Bioquímica Legal; Argentina.
description In our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receive rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72 h after a probed natural runoff event towards the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT) and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-17T15:21:29Z
2023-02-17T15:21:29Z
2023-01
info:eu-repo/date/embargoEnd/2024-01-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14021
https://onlinelibrary.wiley.com/doi/10.1002/em.22529
0893-6692
1098-2280
https://doi.org/10.1002/em.22529
url http://hdl.handle.net/20.500.12123/14021
https://onlinelibrary.wiley.com/doi/10.1002/em.22529
https://doi.org/10.1002/em.22529
identifier_str_mv 0893-6692
1098-2280
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNNAT-1128043/AR./Los agroquímicos como fuente de contaminación difusa en agroecosistemas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Environmental and Molecular Mutagenesis : 1-34 (First published: 31 January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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