Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
- Autores
- Abdala, Alejandra Mariana; Farber, Marisa Diana; Robles, Carlos Alejandro
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.
Estación Experimental Agropecuaria Bariloche
Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina - Fuente
- Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022)
- Materia
-
Enfermedades de los Animales
Enfermedades de la Piel
Ovinos
Animal Diseases
Skin Diseases
Sheep
Región Patagónica
Lana Sisal
Raza Merino - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/13579
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Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesionsAbdala, Alejandra MarianaFarber, Marisa DianaRobles, Carlos AlejandroEnfermedades de los AnimalesEnfermedades de la PielOvinosAnimal DiseasesSkin DiseasesSheepRegión PatagónicaLana SisalRaza MerinoBackground: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.Estación Experimental Agropecuaria BarilocheFil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaFil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaWiley2022-12-12T14:11:04Z2022-12-12T14:11:04Z2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13579https://onlinelibrary.wiley.com/doi/10.1111/vde.131241365-3164https://doi.org/10.1111/vde.13124Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:49Zoai:localhost:20.500.12123/13579instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:50.161INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
title |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
spellingShingle |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions Abdala, Alejandra Mariana Enfermedades de los Animales Enfermedades de la Piel Ovinos Animal Diseases Skin Diseases Sheep Región Patagónica Lana Sisal Raza Merino |
title_short |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
title_full |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
title_fullStr |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
title_full_unstemmed |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
title_sort |
Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions |
dc.creator.none.fl_str_mv |
Abdala, Alejandra Mariana Farber, Marisa Diana Robles, Carlos Alejandro |
author |
Abdala, Alejandra Mariana |
author_facet |
Abdala, Alejandra Mariana Farber, Marisa Diana Robles, Carlos Alejandro |
author_role |
author |
author2 |
Farber, Marisa Diana Robles, Carlos Alejandro |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Enfermedades de los Animales Enfermedades de la Piel Ovinos Animal Diseases Skin Diseases Sheep Región Patagónica Lana Sisal Raza Merino |
topic |
Enfermedades de los Animales Enfermedades de la Piel Ovinos Animal Diseases Skin Diseases Sheep Región Patagónica Lana Sisal Raza Merino |
dc.description.none.fl_txt_mv |
Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy. Estación Experimental Agropecuaria Bariloche Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina Fil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina |
description |
Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-12T14:11:04Z 2022-12-12T14:11:04Z 2022-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/13579 https://onlinelibrary.wiley.com/doi/10.1111/vde.13124 1365-3164 https://doi.org/10.1111/vde.13124 |
url |
http://hdl.handle.net/20.500.12123/13579 https://onlinelibrary.wiley.com/doi/10.1111/vde.13124 https://doi.org/10.1111/vde.13124 |
identifier_str_mv |
1365-3164 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNSA-1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos. |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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