Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions

Autores
Abdala, Alejandra Mariana; Farber, Marisa Diana; Robles, Carlos Alejandro
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.
Estación Experimental Agropecuaria Bariloche
Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fuente
Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022)
Materia
Enfermedades de los Animales
Enfermedades de la Piel
Ovinos
Animal Diseases
Skin Diseases
Sheep
Región Patagónica
Lana Sisal
Raza Merino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13579
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13579
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network_name_str INTA Digital (INTA)
spelling Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesionsAbdala, Alejandra MarianaFarber, Marisa DianaRobles, Carlos AlejandroEnfermedades de los AnimalesEnfermedades de la PielOvinosAnimal DiseasesSkin DiseasesSheepRegión PatagónicaLana SisalRaza MerinoBackground: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.Estación Experimental Agropecuaria BarilocheFil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaFil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaWiley2022-12-12T14:11:04Z2022-12-12T14:11:04Z2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13579https://onlinelibrary.wiley.com/doi/10.1111/vde.131241365-3164https://doi.org/10.1111/vde.13124Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.info:eu-repo/semantics/restrictedAccess2025-10-23T11:18:13Zoai:localhost:20.500.12123/13579instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:13.337INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
title Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
spellingShingle Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
Abdala, Alejandra Mariana
Enfermedades de los Animales
Enfermedades de la Piel
Ovinos
Animal Diseases
Skin Diseases
Sheep
Región Patagónica
Lana Sisal
Raza Merino
title_short Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
title_full Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
title_fullStr Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
title_full_unstemmed Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
title_sort Sisal wool skin disease in Merino sheep in the Argentine Patagonia region: Identification and molecular diagnosis of Corynebacterium bovis from skin lesions
dc.creator.none.fl_str_mv Abdala, Alejandra Mariana
Farber, Marisa Diana
Robles, Carlos Alejandro
author Abdala, Alejandra Mariana
author_facet Abdala, Alejandra Mariana
Farber, Marisa Diana
Robles, Carlos Alejandro
author_role author
author2 Farber, Marisa Diana
Robles, Carlos Alejandro
author2_role author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Enfermedades de la Piel
Ovinos
Animal Diseases
Skin Diseases
Sheep
Región Patagónica
Lana Sisal
Raza Merino
topic Enfermedades de los Animales
Enfermedades de la Piel
Ovinos
Animal Diseases
Skin Diseases
Sheep
Región Patagónica
Lana Sisal
Raza Merino
dc.description.none.fl_txt_mv Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.
Estación Experimental Agropecuaria Bariloche
Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Abdala, Alejandra Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
description Background: Sisal wool condition is a skin disease affecting Merino sheep in the Argentine Patagonia region. Corynebacterium spp. isolates have previously been isolated from skin swabs from lesions, while specific identification of the bacteria involved has not been reported. Hypothesis/Objectives: The aim of this work was to characterize the bacterial agent isolated from sisal wool lesions and to develop a diagnostic method for field surveillance. Materials and Methods: Molecular identification of a collection of 72 isolates obtained previously was performed using PCR and 16S rRNA and rpoB sequencing. A field survey was carried out on two farms in the Río Negro province of Argentine Patagonia. Swab samples from sheep with and without skin lesions were collected and analysed by PCR and culture. Results: Isolates analysed were confirmed by sequencing as Corynebacterium bovis. Using a PCR test without culture step, all field samples from affected sheep were positive for C. bovis; samples from the healthy skin from the same animals or clinically healthy sheep all were negative. Conclusions and clinical relevance: Sisal wool skin disease was associated with C. bovis infection based on culture and PCR methods; the latter may be useful for helping to pursue a disease control strategy.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-12T14:11:04Z
2022-12-12T14:11:04Z
2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13579
https://onlinelibrary.wiley.com/doi/10.1111/vde.13124
1365-3164
https://doi.org/10.1111/vde.13124
url http://hdl.handle.net/20.500.12123/13579
https://onlinelibrary.wiley.com/doi/10.1111/vde.13124
https://doi.org/10.1111/vde.13124
identifier_str_mv 1365-3164
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Veterinary Dermatology 00 : 1–8 (First published: 29 September 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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