Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil

Autores
Dietzgen, Ralf Georg; Bejerman, Nicolas Esteban; Yongyu, Mei; Jee, Charmaine Lim Jing; Chabi-Jesus, Camila; Freitas-Astúa, Juliana; Veras, Solange M.; Kitajima, Elliot W.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
Instituto de Patología Vegetal
Fil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; Brasil
Fil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; Brasil
Fil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; Brasil
Fuente
Archives of Virology 166 : 1615-1622. (2021)
Materia
Solanum
Plant Viruses
Rhabdoviridae
Plant Rhabdoviruses
Brazil
Virus de las Plantas
Rhabdovirus de las Plantas
Brasil
Solanum aculeatissimum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/23082
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network_name_str INTA Digital (INTA)
spelling Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in BrazilDietzgen, Ralf GeorgBejerman, Nicolas EstebanYongyu, MeiJee, Charmaine Lim JingChabi-Jesus, CamilaFreitas-Astúa, JulianaVeras, Solange M.Kitajima, Elliot W.SolanumPlant VirusesRhabdoviridaePlant RhabdovirusesBrazilVirus de las PlantasRhabdovirus de las PlantasBrasilSolanum aculeatissimumWe identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.Instituto de Patología VegetalFil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); BrasilFil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); BrasilFil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; BrasilFil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; BrasilFil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; BrasilSpringer2025-07-18T14:20:06Z2025-07-18T14:20:06Z2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23082https://link.springer.com/article/10.1007/s00705-021-05040-y0304-86081432-8798https://doi.org/10.1007/s00705-021-05040-yArchives of Virology 166 : 1615-1622. (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:25Zoai:localhost:20.500.12123/23082instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:25.553INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
title Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
spellingShingle Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
Dietzgen, Ralf Georg
Solanum
Plant Viruses
Rhabdoviridae
Plant Rhabdoviruses
Brazil
Virus de las Plantas
Rhabdovirus de las Plantas
Brasil
Solanum aculeatissimum
title_short Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
title_full Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
title_fullStr Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
title_full_unstemmed Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
title_sort Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
dc.creator.none.fl_str_mv Dietzgen, Ralf Georg
Bejerman, Nicolas Esteban
Yongyu, Mei
Jee, Charmaine Lim Jing
Chabi-Jesus, Camila
Freitas-Astúa, Juliana
Veras, Solange M.
Kitajima, Elliot W.
author Dietzgen, Ralf Georg
author_facet Dietzgen, Ralf Georg
Bejerman, Nicolas Esteban
Yongyu, Mei
Jee, Charmaine Lim Jing
Chabi-Jesus, Camila
Freitas-Astúa, Juliana
Veras, Solange M.
Kitajima, Elliot W.
author_role author
author2 Bejerman, Nicolas Esteban
Yongyu, Mei
Jee, Charmaine Lim Jing
Chabi-Jesus, Camila
Freitas-Astúa, Juliana
Veras, Solange M.
Kitajima, Elliot W.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Solanum
Plant Viruses
Rhabdoviridae
Plant Rhabdoviruses
Brazil
Virus de las Plantas
Rhabdovirus de las Plantas
Brasil
Solanum aculeatissimum
topic Solanum
Plant Viruses
Rhabdoviridae
Plant Rhabdoviruses
Brazil
Virus de las Plantas
Rhabdovirus de las Plantas
Brasil
Solanum aculeatissimum
dc.description.none.fl_txt_mv We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
Instituto de Patología Vegetal
Fil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; Brasil
Fil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; Brasil
Fil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; Brasil
description We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
publishDate 2021
dc.date.none.fl_str_mv 2021
2025-07-18T14:20:06Z
2025-07-18T14:20:06Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23082
https://link.springer.com/article/10.1007/s00705-021-05040-y
0304-8608
1432-8798
https://doi.org/10.1007/s00705-021-05040-y
url http://hdl.handle.net/20.500.12123/23082
https://link.springer.com/article/10.1007/s00705-021-05040-y
https://doi.org/10.1007/s00705-021-05040-y
identifier_str_mv 0304-8608
1432-8798
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Archives of Virology 166 : 1615-1622. (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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