Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
- Autores
- Dietzgen, Ralf Georg; Bejerman, Nicolas Esteban; Yongyu, Mei; Jee, Charmaine Lim Jing; Chabi-Jesus, Camila; Freitas-Astúa, Juliana; Veras, Solange M.; Kitajima, Elliot W.
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.
Instituto de Patología Vegetal
Fil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; Australia
Fil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); Brasil
Fil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; Brasil
Fil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; Brasil
Fil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; Brasil - Fuente
- Archives of Virology 166 : 1615-1622. (2021)
- Materia
-
Solanum
Plant Viruses
Rhabdoviridae
Plant Rhabdoviruses
Brazil
Virus de las Plantas
Rhabdovirus de las Plantas
Brasil
Solanum aculeatissimum - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/23082
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Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in BrazilDietzgen, Ralf GeorgBejerman, Nicolas EstebanYongyu, MeiJee, Charmaine Lim JingChabi-Jesus, CamilaFreitas-Astúa, JulianaVeras, Solange M.Kitajima, Elliot W.SolanumPlant VirusesRhabdoviridaePlant RhabdovirusesBrazilVirus de las PlantasRhabdovirus de las PlantasBrasilSolanum aculeatissimumWe identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions.Instituto de Patología VegetalFil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; AustraliaFil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); BrasilFil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); BrasilFil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; BrasilFil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; BrasilFil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; BrasilSpringer2025-07-18T14:20:06Z2025-07-18T14:20:06Z2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23082https://link.springer.com/article/10.1007/s00705-021-05040-y0304-86081432-8798https://doi.org/10.1007/s00705-021-05040-yArchives of Virology 166 : 1615-1622. (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:38Zoai:localhost:20.500.12123/23082instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:38.757INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| spellingShingle |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil Dietzgen, Ralf Georg Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum |
| title_short |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_full |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_fullStr |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_full_unstemmed |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_sort |
Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| dc.creator.none.fl_str_mv |
Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. |
| author |
Dietzgen, Ralf Georg |
| author_facet |
Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. |
| author_role |
author |
| author2 |
Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum |
| topic |
Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum |
| dc.description.none.fl_txt_mv |
We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions. Instituto de Patología Vegetal Fil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); Brasil Fil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); Brasil Fil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; Brasil Fil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; Brasil Fil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; Brasil |
| description |
We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2025-07-18T14:20:06Z 2025-07-18T14:20:06Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/23082 https://link.springer.com/article/10.1007/s00705-021-05040-y 0304-8608 1432-8798 https://doi.org/10.1007/s00705-021-05040-y |
| url |
http://hdl.handle.net/20.500.12123/23082 https://link.springer.com/article/10.1007/s00705-021-05040-y https://doi.org/10.1007/s00705-021-05040-y |
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0304-8608 1432-8798 |
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eng |
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eng |
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Springer |
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Springer |
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Archives of Virology 166 : 1615-1622. (2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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