Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
- Autores
- Calvo, Franco Emmanuel; Silvente, Sonia Teresa; Trentacoste, Eduardo Rafael
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.
EEA La Consulta
Fil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); Argentina
Fil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
Fil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina - Fuente
- Sustainability 15 (18) : 13472. (September 2023)
- Materia
-
Juglans regia
Nuez
Variedades
Metabolómica
Estrés de Sequía
Walnuts
Varieties
Metabolomics
Drought Stress
Variedad Chandler
Estrés Hídrico
Water Stress - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/19194
Ver los metadatos del registro completo
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Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. ChandlerCalvo, Franco EmmanuelSilvente, Sonia TeresaTrentacoste, Eduardo RafaelJuglans regiaNuezVariedadesMetabolómicaEstrés de SequíaWalnutsVarietiesMetabolomicsDrought StressVariedad ChandlerEstrés HídricoWater StressWalnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.EEA La ConsultaFil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); ArgentinaFil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); ArgentinaFil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaMDPI2024-08-30T14:56:09Z2024-08-30T14:56:09Z2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19194https://www.mdpi.com/2071-1050/15/18/134722071-1050https://doi.org/10.3390/su151813472Sustainability 15 (18) : 13472. (September 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:50Zoai:localhost:20.500.12123/19194instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:51.107INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
title |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
spellingShingle |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler Calvo, Franco Emmanuel Juglans regia Nuez Variedades Metabolómica Estrés de Sequía Walnuts Varieties Metabolomics Drought Stress Variedad Chandler Estrés Hídrico Water Stress |
title_short |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
title_full |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
title_fullStr |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
title_full_unstemmed |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
title_sort |
Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler |
dc.creator.none.fl_str_mv |
Calvo, Franco Emmanuel Silvente, Sonia Teresa Trentacoste, Eduardo Rafael |
author |
Calvo, Franco Emmanuel |
author_facet |
Calvo, Franco Emmanuel Silvente, Sonia Teresa Trentacoste, Eduardo Rafael |
author_role |
author |
author2 |
Silvente, Sonia Teresa Trentacoste, Eduardo Rafael |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Juglans regia Nuez Variedades Metabolómica Estrés de Sequía Walnuts Varieties Metabolomics Drought Stress Variedad Chandler Estrés Hídrico Water Stress |
topic |
Juglans regia Nuez Variedades Metabolómica Estrés de Sequía Walnuts Varieties Metabolomics Drought Stress Variedad Chandler Estrés Hídrico Water Stress |
dc.description.none.fl_txt_mv |
Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions. EEA La Consulta Fil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); Argentina Fil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina Fil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina |
description |
Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09 2024-08-30T14:56:09Z 2024-08-30T14:56:09Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/19194 https://www.mdpi.com/2071-1050/15/18/13472 2071-1050 https://doi.org/10.3390/su151813472 |
url |
http://hdl.handle.net/20.500.12123/19194 https://www.mdpi.com/2071-1050/15/18/13472 https://doi.org/10.3390/su151813472 |
identifier_str_mv |
2071-1050 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
Sustainability 15 (18) : 13472. (September 2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.712165 |