Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler

Autores
Calvo, Franco Emmanuel; Silvente, Sonia Teresa; Trentacoste, Eduardo Rafael
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.
EEA La Consulta
Fil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); Argentina
Fil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
Fil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fuente
Sustainability 15 (18) : 13472. (September 2023)
Materia
Juglans regia
Nuez
Variedades
Metabolómica
Estrés de Sequía
Walnuts
Varieties
Metabolomics
Drought Stress
Variedad Chandler
Estrés Hídrico
Water Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/19194

id INTADig_26b7b50350c3ab2cf7cb82bef478144d
oai_identifier_str oai:localhost:20.500.12123/19194
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. ChandlerCalvo, Franco EmmanuelSilvente, Sonia TeresaTrentacoste, Eduardo RafaelJuglans regiaNuezVariedadesMetabolómicaEstrés de SequíaWalnutsVarietiesMetabolomicsDrought StressVariedad ChandlerEstrés HídricoWater StressWalnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.EEA La ConsultaFil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); ArgentinaFil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); ArgentinaFil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaMDPI2024-08-30T14:56:09Z2024-08-30T14:56:09Z2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19194https://www.mdpi.com/2071-1050/15/18/134722071-1050https://doi.org/10.3390/su151813472Sustainability 15 (18) : 13472. (September 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:50Zoai:localhost:20.500.12123/19194instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:51.107INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
title Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
spellingShingle Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
Calvo, Franco Emmanuel
Juglans regia
Nuez
Variedades
Metabolómica
Estrés de Sequía
Walnuts
Varieties
Metabolomics
Drought Stress
Variedad Chandler
Estrés Hídrico
Water Stress
title_short Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
title_full Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
title_fullStr Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
title_full_unstemmed Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
title_sort Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler
dc.creator.none.fl_str_mv Calvo, Franco Emmanuel
Silvente, Sonia Teresa
Trentacoste, Eduardo Rafael
author Calvo, Franco Emmanuel
author_facet Calvo, Franco Emmanuel
Silvente, Sonia Teresa
Trentacoste, Eduardo Rafael
author_role author
author2 Silvente, Sonia Teresa
Trentacoste, Eduardo Rafael
author2_role author
author
dc.subject.none.fl_str_mv Juglans regia
Nuez
Variedades
Metabolómica
Estrés de Sequía
Walnuts
Varieties
Metabolomics
Drought Stress
Variedad Chandler
Estrés Hídrico
Water Stress
topic Juglans regia
Nuez
Variedades
Metabolómica
Estrés de Sequía
Walnuts
Varieties
Metabolomics
Drought Stress
Variedad Chandler
Estrés Hídrico
Water Stress
dc.description.none.fl_txt_mv Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.
EEA La Consulta
Fil: Calvo, Franco. Universidad Nacional de Chilecito. Instituto de Agricultura Sostenible en el Oasis (IASO); Argentina
Fil: Calvo, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
Fil: Trentacoste, Eduardo Rafael. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
description Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography–mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
2024-08-30T14:56:09Z
2024-08-30T14:56:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/19194
https://www.mdpi.com/2071-1050/15/18/13472
2071-1050
https://doi.org/10.3390/su151813472
url http://hdl.handle.net/20.500.12123/19194
https://www.mdpi.com/2071-1050/15/18/13472
https://doi.org/10.3390/su151813472
identifier_str_mv 2071-1050
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Sustainability 15 (18) : 13472. (September 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143578231799808
score 12.712165