Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky

Autores
Carossino, Mariano; Barrandeguy, Maria Edith; Li, Yanqiu; Parreño, Viviana; Janes, Jennifer; Loynachan, Alan T.; Balasuriya, Udeni B.R.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.
Instituto de Virología
Fil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Li, Yanqiu. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Janes, Jennifer. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Loynachan, Alan T. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Balasuriya, Udeni B.R. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina
Fuente
Virus research 255 : 39-54. (15 August 2018)
Materia
Rotavirus
Caballos
Filogenia
Horses
Phylogeny
Foal Diarrhea
G3P
G14P
Equine Rotavirus
Kentucky, Estados Unidos
Diarrea del Potro
Rotavirus Equino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4448
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4448
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network_name_str INTA Digital (INTA)
spelling Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central KentuckyCarossino, MarianoBarrandeguy, Maria EdithLi, YanqiuParreño, VivianaJanes, JenniferLoynachan, Alan T.Balasuriya, Udeni B.R.RotavirusCaballosFilogeniaHorsesPhylogenyFoal DiarrheaG3PG14PEquine RotavirusKentucky, Estados UnidosDiarrea del PotroRotavirus EquinoEquine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.Instituto de VirologíaFil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; ArgentinaFil: Li, Yanqiu. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados UnidosFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Janes, Jennifer. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados UnidosFil: Loynachan, Alan T. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados UnidosFil: Balasuriya, Udeni B.R. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; ArgentinaElsevier2019-02-15T13:35:42Z2019-02-15T13:35:42Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4448https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub0168-1702https://doi.org/10.1016/j.virusres.2018.05.025Virus research 255 : 39-54. (15 August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:48Zoai:localhost:20.500.12123/4448instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:49.049INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
title Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
spellingShingle Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
Carossino, Mariano
Rotavirus
Caballos
Filogenia
Horses
Phylogeny
Foal Diarrhea
G3P
G14P
Equine Rotavirus
Kentucky, Estados Unidos
Diarrea del Potro
Rotavirus Equino
title_short Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
title_full Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
title_fullStr Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
title_full_unstemmed Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
title_sort Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
dc.creator.none.fl_str_mv Carossino, Mariano
Barrandeguy, Maria Edith
Li, Yanqiu
Parreño, Viviana
Janes, Jennifer
Loynachan, Alan T.
Balasuriya, Udeni B.R.
author Carossino, Mariano
author_facet Carossino, Mariano
Barrandeguy, Maria Edith
Li, Yanqiu
Parreño, Viviana
Janes, Jennifer
Loynachan, Alan T.
Balasuriya, Udeni B.R.
author_role author
author2 Barrandeguy, Maria Edith
Li, Yanqiu
Parreño, Viviana
Janes, Jennifer
Loynachan, Alan T.
Balasuriya, Udeni B.R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Rotavirus
Caballos
Filogenia
Horses
Phylogeny
Foal Diarrhea
G3P
G14P
Equine Rotavirus
Kentucky, Estados Unidos
Diarrea del Potro
Rotavirus Equino
topic Rotavirus
Caballos
Filogenia
Horses
Phylogeny
Foal Diarrhea
G3P
G14P
Equine Rotavirus
Kentucky, Estados Unidos
Diarrea del Potro
Rotavirus Equino
dc.description.none.fl_txt_mv Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.
Instituto de Virología
Fil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina
Fil: Li, Yanqiu. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Janes, Jennifer. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Loynachan, Alan T. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Balasuriya, Udeni B.R. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina
description Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-02-15T13:35:42Z
2019-02-15T13:35:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4448
https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub
0168-1702
https://doi.org/10.1016/j.virusres.2018.05.025
url http://hdl.handle.net/20.500.12123/4448
https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub
https://doi.org/10.1016/j.virusres.2018.05.025
identifier_str_mv 0168-1702
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Virus research 255 : 39-54. (15 August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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