Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding

Autores
Almasia, Natalia Ines; Nahirñak, Vanesa; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.
Instituto de Biotecnología
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Electronic journal of biotechnology 13 (5) : 1-21. (Septiembre 2010)
Materia
Peptides
Antimicrobials
Potatoes
Genetic Isolation
Temperature
Transgenic Plants
Péptidos
Antimicrobianos
Papa
Solanum Tuberosum
Aislamiento Genético
Temperatura
Plantas Transgénicas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8640
Acceder
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network_name_str INTA Digital (INTA)
spelling Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and woundingAlmasia, Natalia InesNahirñak, VanesaHopp, Horacio EstebanVazquez Rovere, CeciliaPeptidesAntimicrobialsPotatoesGenetic IsolationTemperatureTransgenic PlantsPéptidosAntimicrobianosPapaSolanum TuberosumAislamiento GenéticoTemperaturaPlantas TransgénicasSnakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.Instituto de BiotecnologíaFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaPontificia Universidad Catolica de Valparaiso2021-01-22T18:01:10Z2021-01-22T18:01:10Z2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8640https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso0717-345810.2225/vol13-issue5-fulltext-12Electronic journal of biotechnology 13 (5) : 1-21. (Septiembre 2010)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:45Zoai:localhost:20.500.12123/8640instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:46.078INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
spellingShingle Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
Almasia, Natalia Ines
Peptides
Antimicrobials
Potatoes
Genetic Isolation
Temperature
Transgenic Plants
Péptidos
Antimicrobianos
Papa
Solanum Tuberosum
Aislamiento Genético
Temperatura
Plantas Transgénicas
title_short Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_full Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_fullStr Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_full_unstemmed Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_sort Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
dc.creator.none.fl_str_mv Almasia, Natalia Ines
Nahirñak, Vanesa
Hopp, Horacio Esteban
Vazquez Rovere, Cecilia
author Almasia, Natalia Ines
author_facet Almasia, Natalia Ines
Nahirñak, Vanesa
Hopp, Horacio Esteban
Vazquez Rovere, Cecilia
author_role author
author2 Nahirñak, Vanesa
Hopp, Horacio Esteban
Vazquez Rovere, Cecilia
author2_role author
author
author
dc.subject.none.fl_str_mv Peptides
Antimicrobials
Potatoes
Genetic Isolation
Temperature
Transgenic Plants
Péptidos
Antimicrobianos
Papa
Solanum Tuberosum
Aislamiento Genético
Temperatura
Plantas Transgénicas
topic Peptides
Antimicrobials
Potatoes
Genetic Isolation
Temperature
Transgenic Plants
Péptidos
Antimicrobianos
Papa
Solanum Tuberosum
Aislamiento Genético
Temperatura
Plantas Transgénicas
dc.description.none.fl_txt_mv Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.
Instituto de Biotecnología
Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
2021-01-22T18:01:10Z
2021-01-22T18:01:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8640
https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso
0717-3458
10.2225/vol13-issue5-fulltext-12
url http://hdl.handle.net/20.500.12123/8640
https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso
identifier_str_mv 0717-3458
10.2225/vol13-issue5-fulltext-12
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaiso
publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaiso
dc.source.none.fl_str_mv Electronic journal of biotechnology 13 (5) : 1-21. (Septiembre 2010)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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