Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri

Autores
Rybak, Myrian Asucena
Año de publicación
2005
Idioma
inglés
Tipo de recurso
tesis doctoral
Estado
versión aceptada
Colaborador/a o director/a de tesis
Jones, Jeffrey B.
Descripción
Tesis para obtener el grado de Doctor (PhD), de la University of Florida, en 2005
A new strain of the citrus canker bacterium (Xanthomonas axonopodis pv. citri) was detected in Florida. It was significant in that it was primarily pathogenic on Key lime trees, but not on grapefruit trees. This strain has been designated as Xac-Aw. This strain has caused concern among regulators with regard to how to treat this bacterium in the eradication program. Of major concern was the stability of the bacterium in regard to host specificity of the strain; for example, could the strain mutate to attack grapefruit and other citrus plants. We investigated the frequency of the development of mutants, the transfer of genes by conjugation, and the presence of an avirulence gene to grapefruit in the genome. We never were able to find a mutant, either natural or induced, that changed host range. In conjugation studies, transfer of marker genes on the chromosome to and from the Xac-Aw strain by conjugation was not successful. In an attempt to locate possible avirulence genes, a genetic library of Xac-Aw DNA was made using the pLAFR vector and transformed into Escherichia coli. This library was transferred to strain 91-118 of X. perforans, which is pathogenic to tomato, but causes a null reaction in grapefruit leaves. The transconjugants were screened for HR in grapefruit leaves. The inoculated leaves were observed for development of an HR in the infiltrated area. In the screening of the Xac-Aw genomic library we found an avirulence gene in the genome of Xac-Aw that interacts with grapefruit leaves to cause an HR. This is the first report of an avirulence gene in the genome of a citrus canker bacterium that interacts with a citrus species. This gene was designated as avrGf1, was sequenced, and then was characterized. The possibility of a second avr gene exists in the Xac-Aw strain, because the Xac-AwΩ lacking a functional avrGf1 did not cause exactly the same symptoms and develop the same population as the wild-type Xac-A strain when it was inoculated into grapefruit leaves.
EEA Cerro Azul
Fil: Rybak, Myrian Asucena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Materia
Citrus
Enfermedades de las Plantas
Xanthomonas axonopodis
Genética
Rango del Huésped
Secuencia Nucleotídica
Marcadores Genéticos
Plant Diseases
Genetics
Host Range
Nucleotide Sequence
Genetic Markers
Xanthomonas axonopodis pv. citri
Cancro de los Cítricos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6644

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spelling Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citriRybak, Myrian AsucenaCitrusEnfermedades de las PlantasXanthomonas axonopodisGenéticaRango del HuéspedSecuencia NucleotídicaMarcadores GenéticosPlant DiseasesGeneticsHost RangeNucleotide SequenceGenetic MarkersXanthomonas axonopodis pv. citriCancro de los CítricosTesis para obtener el grado de Doctor (PhD), de la University of Florida, en 2005A new strain of the citrus canker bacterium (Xanthomonas axonopodis pv. citri) was detected in Florida. It was significant in that it was primarily pathogenic on Key lime trees, but not on grapefruit trees. This strain has been designated as Xac-Aw. This strain has caused concern among regulators with regard to how to treat this bacterium in the eradication program. Of major concern was the stability of the bacterium in regard to host specificity of the strain; for example, could the strain mutate to attack grapefruit and other citrus plants. We investigated the frequency of the development of mutants, the transfer of genes by conjugation, and the presence of an avirulence gene to grapefruit in the genome. We never were able to find a mutant, either natural or induced, that changed host range. In conjugation studies, transfer of marker genes on the chromosome to and from the Xac-Aw strain by conjugation was not successful. In an attempt to locate possible avirulence genes, a genetic library of Xac-Aw DNA was made using the pLAFR vector and transformed into Escherichia coli. This library was transferred to strain 91-118 of X. perforans, which is pathogenic to tomato, but causes a null reaction in grapefruit leaves. The transconjugants were screened for HR in grapefruit leaves. The inoculated leaves were observed for development of an HR in the infiltrated area. In the screening of the Xac-Aw genomic library we found an avirulence gene in the genome of Xac-Aw that interacts with grapefruit leaves to cause an HR. This is the first report of an avirulence gene in the genome of a citrus canker bacterium that interacts with a citrus species. This gene was designated as avrGf1, was sequenced, and then was characterized. The possibility of a second avr gene exists in the Xac-Aw strain, because the Xac-AwΩ lacking a functional avrGf1 did not cause exactly the same symptoms and develop the same population as the wild-type Xac-A strain when it was inoculated into grapefruit leaves.EEA Cerro AzulFil: Rybak, Myrian Asucena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaUniversity of FloridaJones, Jeffrey B.2020-01-09T13:22:12Z2020-01-09T13:22:12Z2005info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttp://hdl.handle.net/20.500.12123/6644https://ufdcimages.uflib.ufl.edu/uf/e0/01/15/22/00001/rybak_m.pdfenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuaria2025-09-29T13:44:52Zoai:localhost:20.500.12123/6644instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.303INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
title Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
spellingShingle Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
Rybak, Myrian Asucena
Citrus
Enfermedades de las Plantas
Xanthomonas axonopodis
Genética
Rango del Huésped
Secuencia Nucleotídica
Marcadores Genéticos
Plant Diseases
Genetics
Host Range
Nucleotide Sequence
Genetic Markers
Xanthomonas axonopodis pv. citri
Cancro de los Cítricos
title_short Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
title_full Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
title_fullStr Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
title_full_unstemmed Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
title_sort Genetic Determinants of Host Range Specificity of the Wellington Strain of Xanthomonas axonopodis pv. citri
dc.creator.none.fl_str_mv Rybak, Myrian Asucena
author Rybak, Myrian Asucena
author_facet Rybak, Myrian Asucena
author_role author
dc.contributor.none.fl_str_mv Jones, Jeffrey B.
dc.subject.none.fl_str_mv Citrus
Enfermedades de las Plantas
Xanthomonas axonopodis
Genética
Rango del Huésped
Secuencia Nucleotídica
Marcadores Genéticos
Plant Diseases
Genetics
Host Range
Nucleotide Sequence
Genetic Markers
Xanthomonas axonopodis pv. citri
Cancro de los Cítricos
topic Citrus
Enfermedades de las Plantas
Xanthomonas axonopodis
Genética
Rango del Huésped
Secuencia Nucleotídica
Marcadores Genéticos
Plant Diseases
Genetics
Host Range
Nucleotide Sequence
Genetic Markers
Xanthomonas axonopodis pv. citri
Cancro de los Cítricos
dc.description.none.fl_txt_mv Tesis para obtener el grado de Doctor (PhD), de la University of Florida, en 2005
A new strain of the citrus canker bacterium (Xanthomonas axonopodis pv. citri) was detected in Florida. It was significant in that it was primarily pathogenic on Key lime trees, but not on grapefruit trees. This strain has been designated as Xac-Aw. This strain has caused concern among regulators with regard to how to treat this bacterium in the eradication program. Of major concern was the stability of the bacterium in regard to host specificity of the strain; for example, could the strain mutate to attack grapefruit and other citrus plants. We investigated the frequency of the development of mutants, the transfer of genes by conjugation, and the presence of an avirulence gene to grapefruit in the genome. We never were able to find a mutant, either natural or induced, that changed host range. In conjugation studies, transfer of marker genes on the chromosome to and from the Xac-Aw strain by conjugation was not successful. In an attempt to locate possible avirulence genes, a genetic library of Xac-Aw DNA was made using the pLAFR vector and transformed into Escherichia coli. This library was transferred to strain 91-118 of X. perforans, which is pathogenic to tomato, but causes a null reaction in grapefruit leaves. The transconjugants were screened for HR in grapefruit leaves. The inoculated leaves were observed for development of an HR in the infiltrated area. In the screening of the Xac-Aw genomic library we found an avirulence gene in the genome of Xac-Aw that interacts with grapefruit leaves to cause an HR. This is the first report of an avirulence gene in the genome of a citrus canker bacterium that interacts with a citrus species. This gene was designated as avrGf1, was sequenced, and then was characterized. The possibility of a second avr gene exists in the Xac-Aw strain, because the Xac-AwΩ lacking a functional avrGf1 did not cause exactly the same symptoms and develop the same population as the wild-type Xac-A strain when it was inoculated into grapefruit leaves.
EEA Cerro Azul
Fil: Rybak, Myrian Asucena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
description Tesis para obtener el grado de Doctor (PhD), de la University of Florida, en 2005
publishDate 2005
dc.date.none.fl_str_mv 2005
2020-01-09T13:22:12Z
2020-01-09T13:22:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/acceptedVersion
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info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6644
https://ufdcimages.uflib.ufl.edu/uf/e0/01/15/22/00001/rybak_m.pdf
url http://hdl.handle.net/20.500.12123/6644
https://ufdcimages.uflib.ufl.edu/uf/e0/01/15/22/00001/rybak_m.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv University of Florida
publisher.none.fl_str_mv University of Florida
dc.source.none.fl_str_mv reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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