Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol

Autores
Salas, Augusto; Ortiz, Leila Melina; Niz, José María; Magariños, Francisco; Del Valle, Eleodoro Eduardo; Achinelly, María Fernanda; Sauka, Diego Hernan
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA) (G.V. al IABIMO); Argentina
Fil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Magariños, Francisco. Hospital Interzonal General de Agudos “Luisa C. de Gandulfo”; Argentina
Fil: Del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. ICIAGRO Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. ICIAGRO Litoral; Argentina
Fil: Achinelly, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Achinelly, María Fernanda. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Biocontrol Science and Technology 35 (7) : 822-837 (June 2025)
Materia
Biological Control
Control Biológico
Serratia
Meloidogyne incognita
Panagrellus
Heterorhabditis bacteriophora
Serratia odorifera
Panagrellus redivivus
serrawettins
serralysin
Nemátodos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrolSalas, AugustoOrtiz, Leila MelinaNiz, José MaríaMagariños, FranciscoDel Valle, Eleodoro EduardoAchinelly, María FernandaSauka, Diego HernanBiological ControlControl BiológicoSerratiaMeloidogyne incognitaPanagrellusHeterorhabditis bacteriophoraSerratia odoriferaPanagrellus redivivusserrawettinsserralysinNemátodosThe increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA) (G.V. al IABIMO); ArgentinaFil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Magariños, Francisco. Hospital Interzonal General de Agudos “Luisa C. de Gandulfo”; ArgentinaFil: Del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. ICIAGRO Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. ICIAGRO Litoral; ArgentinaFil: Achinelly, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Achinelly, María Fernanda. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2025-10-30T10:30:01Z2025-10-30T10:30:01Z2025-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24379https://www.tandfonline.com/doi/10.1080/09583157.2025.25202500958-31571360-0478https://doi.org/10.1080/09583157.2025.2520250Biocontrol Science and Technology 35 (7) : 822-837 (June 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-06T09:42:59Zoai:localhost:20.500.12123/24379instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:42:59.423INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
title Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
spellingShingle Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
Salas, Augusto
Biological Control
Control Biológico
Serratia
Meloidogyne incognita
Panagrellus
Heterorhabditis bacteriophora
Serratia odorifera
Panagrellus redivivus
serrawettins
serralysin
Nemátodos
title_short Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
title_full Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
title_fullStr Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
title_full_unstemmed Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
title_sort Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
dc.creator.none.fl_str_mv Salas, Augusto
Ortiz, Leila Melina
Niz, José María
Magariños, Francisco
Del Valle, Eleodoro Eduardo
Achinelly, María Fernanda
Sauka, Diego Hernan
author Salas, Augusto
author_facet Salas, Augusto
Ortiz, Leila Melina
Niz, José María
Magariños, Francisco
Del Valle, Eleodoro Eduardo
Achinelly, María Fernanda
Sauka, Diego Hernan
author_role author
author2 Ortiz, Leila Melina
Niz, José María
Magariños, Francisco
Del Valle, Eleodoro Eduardo
Achinelly, María Fernanda
Sauka, Diego Hernan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biological Control
Control Biológico
Serratia
Meloidogyne incognita
Panagrellus
Heterorhabditis bacteriophora
Serratia odorifera
Panagrellus redivivus
serrawettins
serralysin
Nemátodos
topic Biological Control
Control Biológico
Serratia
Meloidogyne incognita
Panagrellus
Heterorhabditis bacteriophora
Serratia odorifera
Panagrellus redivivus
serrawettins
serralysin
Nemátodos
dc.description.none.fl_txt_mv The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA) (G.V. al IABIMO); Argentina
Fil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Magariños, Francisco. Hospital Interzonal General de Agudos “Luisa C. de Gandulfo”; Argentina
Fil: Del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. ICIAGRO Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. ICIAGRO Litoral; Argentina
Fil: Achinelly, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Achinelly, María Fernanda. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-30T10:30:01Z
2025-10-30T10:30:01Z
2025-06-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24379
https://www.tandfonline.com/doi/10.1080/09583157.2025.2520250
0958-3157
1360-0478
https://doi.org/10.1080/09583157.2025.2520250
url http://hdl.handle.net/20.500.12123/24379
https://www.tandfonline.com/doi/10.1080/09583157.2025.2520250
https://doi.org/10.1080/09583157.2025.2520250
identifier_str_mv 0958-3157
1360-0478
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Biocontrol Science and Technology 35 (7) : 822-837 (June 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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