Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies

Autores
Soldati, Maria Cristina; Inza, Maria Virginia; Fornes, Luis Fernando; Zelener, Noga
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela species
Instituto de Recursos Biológicos
Fil: Soldati, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Inza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Fornes, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Zelener, Noga. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fuente
Biochemical systematics and ecology 53 : 8-16. (April 2014)
Materia
Cedrela
Genética de Poblaciones
Especies en Peligro de Extinción
Meliaceae
Microsatélites
Population Genetics
Endangered Species
Microsatellites
Transferability
Cross-species
Transferibilidad
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studiesSoldati, Maria CristinaInza, Maria VirginiaFornes, Luis FernandoZelener, NogaCedrelaGenética de PoblacionesEspecies en Peligro de ExtinciónMeliaceaeMicrosatélitesPopulation GeneticsEndangered SpeciesMicrosatellitesTransferabilityCross-speciesTransferibilidadSpecies of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela speciesInstituto de Recursos BiológicosFil: Soldati, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Inza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Fornes, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Zelener, Noga. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaElsevier2018-10-17T16:32:52Z2018-10-17T16:32:52Z2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3617https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub0305-1978https://doi.org/10.1016/j.bse.2013.12.003Biochemical systematics and ecology 53 : 8-16. (April 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3617instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:28.388INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
title Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
spellingShingle Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
Soldati, Maria Cristina
Cedrela
Genética de Poblaciones
Especies en Peligro de Extinción
Meliaceae
Microsatélites
Population Genetics
Endangered Species
Microsatellites
Transferability
Cross-species
Transferibilidad
title_short Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
title_full Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
title_fullStr Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
title_full_unstemmed Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
title_sort Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
dc.creator.none.fl_str_mv Soldati, Maria Cristina
Inza, Maria Virginia
Fornes, Luis Fernando
Zelener, Noga
author Soldati, Maria Cristina
author_facet Soldati, Maria Cristina
Inza, Maria Virginia
Fornes, Luis Fernando
Zelener, Noga
author_role author
author2 Inza, Maria Virginia
Fornes, Luis Fernando
Zelener, Noga
author2_role author
author
author
dc.subject.none.fl_str_mv Cedrela
Genética de Poblaciones
Especies en Peligro de Extinción
Meliaceae
Microsatélites
Population Genetics
Endangered Species
Microsatellites
Transferability
Cross-species
Transferibilidad
topic Cedrela
Genética de Poblaciones
Especies en Peligro de Extinción
Meliaceae
Microsatélites
Population Genetics
Endangered Species
Microsatellites
Transferability
Cross-species
Transferibilidad
dc.description.none.fl_txt_mv Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela species
Instituto de Recursos Biológicos
Fil: Soldati, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Inza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Fornes, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Zelener, Noga. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
description Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela species
publishDate 2014
dc.date.none.fl_str_mv 2014-04
2018-10-17T16:32:52Z
2018-10-17T16:32:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3617
https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub
0305-1978
https://doi.org/10.1016/j.bse.2013.12.003
url http://hdl.handle.net/20.500.12123/3617
https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub
https://doi.org/10.1016/j.bse.2013.12.003
identifier_str_mv 0305-1978
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biochemical systematics and ecology 53 : 8-16. (April 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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