Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure

• Autores: Young, Brian Jonathan; Cristos, Diego Sebastian; Crespo, Diana Cristina; Somoza, Gustavo Manuel; Carriquiriborde, Pedro
• Año de publicación: 2020
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Ecotoxicology and Environmental Safety 205 : art. 111176. (2020).
• Materia: Melanins; Genetic Markers; Melaninas; Marcadores Genéticos; Endocrine Disruption; Xenoestrogen; Secondary Sexual Characteristics; Biomarker; Alteración Endocrina; Xenoestrógeno; Características Sexuales Secundarias; Biomarcador; Teleostei
• Institución: Instituto Nacional de Tecnología Agropecuaria

The effects of 17α-ethinylestradiol (EE2) on sex ratio, gonopodium morphology, and gonadal histology of C. decemmaculatus were assessed by a full-lifecycle exposure experiment. Newborn fish were waterborne exposed to 30, 100, and 300 ng EE2/L for 90 d, using 50 fish per treatment. Additionally, in December of 2016, a field survey was conducted on a C. decemmaculatus population inhabiting the Girado Creek downstream of the Chascomus city wastewater effluent discharge. After 90 d of exposure, EE2 was able to histologically skew the sex ratio toward females and inhibit the full gonopodium development since the lowest tested concentration (LOEC = 30 ng/L). At higher concentrations, EE2 was toxic, inducing mortality in a concentration-dependent fashion (90 d-LC50 = 109.9 ng/L) and altering the gonadal histoarchitecture, causing neither testes nor ovaries discernible histologically (LOEC = 100 ng/L). In addition, a novel response, perianal hyperpigmentation, was discovered been induced by the EE2 exposure in a concentration-dependent fashion (90 d-EC50 = 39.3 ng/L). A higher proportion of females and perianal hyperpigmentation were observed in wild fish collected from the Girado Creek. The major reached conclusions are: i) EE2 induce different effects on the sexual traits of C. decemmaculatus when exposed from early-life or adult stages. ii) The most sensitive effects observed in the laboratory occur in a creek receiving wastewater effluent. iii) The perianal hyperpigmentation comes-up as a promising biomarker of exposure to estrogenic compounds.
IMyZA
Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina.
Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.
Fil: Crespo, Diana Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina.
Fil: Somoza, Gustavo Manuel. Instituto Tecnológico de Chascomús (CONICET-UNSAM); Argentina.
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones Del Medioambiente. Facultad de Ciencias Exactas; Argentina.