Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies

Autores
Behlau, Franklin; Gochez, Alberto Martin; Lugo, Alshia Janel; Elibox, Winston; Minsavage, Gerald V.; Potnis, Neha; White, Frank F.; Ebrahim, Mohamed; Jones, Jeffrey B.; Ramsubhag, Adesh
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.
EEA Bella Vista
Fil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; Brasil
Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina
Fil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
Fil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados Unidos
Fil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados Unidos
Fil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; Egipto
Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
Fuente
European Journal of Plant Pathology 147 (3) : 671–681 (March 2017)
Materia
Xanthomonas campestris
Genética
Enfermedades de las Plantas
Cobre
Filogenia
Genomas
Genetics
Plant Diseases
Copper
Phylogeny
Genomes
Trinidad y Tobago
Xanthomonas campestris pv. campestris
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4150
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oai_identifier_str oai:localhost:20.500.12123/4150
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West IndiesBehlau, FranklinGochez, Alberto MartinLugo, Alshia JanelElibox, WinstonMinsavage, Gerald V.Potnis, NehaWhite, Frank F.Ebrahim, MohamedJones, Jeffrey B.Ramsubhag, AdeshXanthomonas campestrisGenéticaEnfermedades de las PlantasCobreFilogeniaGenomasGeneticsPlant DiseasesCopperPhylogenyGenomesTrinidad y TobagoXanthomonas campestris pv. campestrisWhole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.EEA Bella VistaFil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; BrasilFil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y TobagoFil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y TobagoFil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados UnidosFil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados UnidosFil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados UnidosFil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; EgiptoFil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados UnidosFil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y TobagoSpringer2018-12-26T16:54:48Z2018-12-26T16:54:48Z2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s10658-016-1035-2http://hdl.handle.net/20.500.12123/41500929-18731573-8469https://doi.org/10.1007/s10658-016-1035-2European Journal of Plant Pathology 147 (3) : 671–681 (March 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:44Zoai:localhost:20.500.12123/4150instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:45.281INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
title Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
spellingShingle Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
Behlau, Franklin
Xanthomonas campestris
Genética
Enfermedades de las Plantas
Cobre
Filogenia
Genomas
Genetics
Plant Diseases
Copper
Phylogeny
Genomes
Trinidad y Tobago
Xanthomonas campestris pv. campestris
title_short Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
title_full Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
title_fullStr Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
title_full_unstemmed Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
title_sort Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
dc.creator.none.fl_str_mv Behlau, Franklin
Gochez, Alberto Martin
Lugo, Alshia Janel
Elibox, Winston
Minsavage, Gerald V.
Potnis, Neha
White, Frank F.
Ebrahim, Mohamed
Jones, Jeffrey B.
Ramsubhag, Adesh
author Behlau, Franklin
author_facet Behlau, Franklin
Gochez, Alberto Martin
Lugo, Alshia Janel
Elibox, Winston
Minsavage, Gerald V.
Potnis, Neha
White, Frank F.
Ebrahim, Mohamed
Jones, Jeffrey B.
Ramsubhag, Adesh
author_role author
author2 Gochez, Alberto Martin
Lugo, Alshia Janel
Elibox, Winston
Minsavage, Gerald V.
Potnis, Neha
White, Frank F.
Ebrahim, Mohamed
Jones, Jeffrey B.
Ramsubhag, Adesh
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Xanthomonas campestris
Genética
Enfermedades de las Plantas
Cobre
Filogenia
Genomas
Genetics
Plant Diseases
Copper
Phylogeny
Genomes
Trinidad y Tobago
Xanthomonas campestris pv. campestris
topic Xanthomonas campestris
Genética
Enfermedades de las Plantas
Cobre
Filogenia
Genomas
Genetics
Plant Diseases
Copper
Phylogeny
Genomes
Trinidad y Tobago
Xanthomonas campestris pv. campestris
dc.description.none.fl_txt_mv Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.
EEA Bella Vista
Fil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; Brasil
Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina
Fil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
Fil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados Unidos
Fil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados Unidos
Fil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; Egipto
Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago
description Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
2018-12-26T16:54:48Z
2018-12-26T16:54:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s10658-016-1035-2
http://hdl.handle.net/20.500.12123/4150
0929-1873
1573-8469
https://doi.org/10.1007/s10658-016-1035-2
url https://link.springer.com/article/10.1007/s10658-016-1035-2
http://hdl.handle.net/20.500.12123/4150
https://doi.org/10.1007/s10658-016-1035-2
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology 147 (3) : 671–681 (March 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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