Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno

Autores
Rodríguez, Vanina Andrea; Belaich, Mariano Nicolás; Mengual Gómez, Diego Luis; Sciocco, Alicia Ines; Ghiringhelli, Pablo Daniel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Rodríguez, Vanina Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Rodríguez, Vanina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Belaich, Mariano Nicolás. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Belaich, Mariano Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fuente
Journal of Invertebrate Pathology 106 (2) : 255-262. (Febrero 2011)
Materia
Control biológico de plagas
Biological pest control
Lepidoptera
Virus
Viruses
Nymphalidae
Dione juno
Agraulis vanillae
Butterflies
Nucleopolyhedrovirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21110
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21110
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network_name_str INTA Digital (INTA)
spelling Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione junoRodríguez, Vanina AndreaBelaich, Mariano NicolásMengual Gómez, Diego LuisSciocco, Alicia InesGhiringhelli, Pablo DanielControl biológico de plagasBiological pest controlLepidopteraVirusVirusesNymphalidaeDione junoAgraulis vanillaeButterfliesNucleopolyhedrovirusDione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Rodríguez, Vanina Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; ArgentinaFil: Rodríguez, Vanina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Belaich, Mariano Nicolás. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; ArgentinaFil: Belaich, Mariano Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; ArgentinaFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; ArgentinaElsevier2025-01-31T13:50:53Z2025-01-31T13:50:53Z2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21110Journal of Invertebrate Pathology 106 (2) : 255-262 (Febrero 2011)0022-20111096-0805 (on line)Journal of Invertebrate Pathology 106 (2) : 255-262. (Febrero 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:07Zoai:localhost:20.500.12123/21110instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:07.441INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
title Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
spellingShingle Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
Rodríguez, Vanina Andrea
Control biológico de plagas
Biological pest control
Lepidoptera
Virus
Viruses
Nymphalidae
Dione juno
Agraulis vanillae
Butterflies
Nucleopolyhedrovirus
title_short Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
title_full Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
title_fullStr Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
title_full_unstemmed Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
title_sort Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
dc.creator.none.fl_str_mv Rodríguez, Vanina Andrea
Belaich, Mariano Nicolás
Mengual Gómez, Diego Luis
Sciocco, Alicia Ines
Ghiringhelli, Pablo Daniel
author Rodríguez, Vanina Andrea
author_facet Rodríguez, Vanina Andrea
Belaich, Mariano Nicolás
Mengual Gómez, Diego Luis
Sciocco, Alicia Ines
Ghiringhelli, Pablo Daniel
author_role author
author2 Belaich, Mariano Nicolás
Mengual Gómez, Diego Luis
Sciocco, Alicia Ines
Ghiringhelli, Pablo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Control biológico de plagas
Biological pest control
Lepidoptera
Virus
Viruses
Nymphalidae
Dione juno
Agraulis vanillae
Butterflies
Nucleopolyhedrovirus
topic Control biológico de plagas
Biological pest control
Lepidoptera
Virus
Viruses
Nymphalidae
Dione juno
Agraulis vanillae
Butterflies
Nucleopolyhedrovirus
dc.description.none.fl_txt_mv Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Rodríguez, Vanina Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Rodríguez, Vanina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Belaich, Mariano Nicolás. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Belaich, Mariano Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina
description Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2025-01-31T13:50:53Z
2025-01-31T13:50:53Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21110
Journal of Invertebrate Pathology 106 (2) : 255-262 (Febrero 2011)
0022-2011
1096-0805 (on line)
url http://hdl.handle.net/20.500.12123/21110
identifier_str_mv Journal of Invertebrate Pathology 106 (2) : 255-262 (Febrero 2011)
0022-2011
1096-0805 (on line)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Invertebrate Pathology 106 (2) : 255-262. (Febrero 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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