Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum

• Autores: Vila Aiub, Martín Miguel; Yu, Qin; Han, Heping; Powles, Stephen B.
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of Experimental Botany; Vol.66, no.15; 4711-4718; https://academic.oup.com/jxb
• Materia: ACCASE ACTIVITY; ALOPECURUS MYOSUROIDES; COMPETITION; RESISTANCE COST; RESISTANCE MUTATION; RGR
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Vila Aiub, Martín Miguel. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative (AHRI). WA, Australia.
Fil: Vila Aiub, Martín Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Vila Aiub, Martín Miguel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Yu, Qin. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative (AHRI). WA, Australia.
Fil: Han, Heping. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative (AHRI). WA, Australia.
Fil: Powles, Stephen B. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative (AHRI). WA, Australia.
The rate of herbicide resistance evolution in plants depends on fitness traits endowed by alleles in both the presence and absence (resistance cost) of herbicide selection. The effect of two Lolium rigidum spontaneous homozygous target-site resistance-endowing mutations (Ile-1781-Leu, Asp-2078-Gly) on both ACCase activity and various plant growth traits have been investigated here. Relative growth rate (RGR) and components (net assimilation rate, leaf area ratio), resource allocation to different organs, and growth responses in competition with a wheat crop were assessed. Unlike plants carrying the Ile-1781-Leu resistance mutation, plants homozygous for the Asp-2078-Gly mutation exhibited a significantly lower RGR (30 per cent), which translated into lower allocation of biomass to roots, shoots, and leaves, and poor responses to plant competition. Both the negligible and significant growth reductions associated, respectively, with the Ile-1781-Leu and Asp-2078-Gly resistance mutations correlated with their impact on ACCase activity. Whereas the Ile-1781-Leu mutation showed no pleiotropic effects on ACCase kinetics, the Asp-2078-Gly mutation led to a significant reduction in ACCase activity. The impaired growth traits are discussed in the context of resistance costs and the effects of each resistance allele on ACCase activity. Similar effects of these two particular ACCase mutations on the ACCase activity of Alopecurus myosuroides were also confirmed.