Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels

Autores
Duque Rodríguez, Matteo; Cittadini, Camila O.; Teplitz, Gabriela M.; De Stefano, Adrián Ariel; Lombardo, Daniel M.; Salamone, Daniel Felipe
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.
tbls., grafs., fot.
Fuente
Frontiers in cell and developmental biology
Vol.9
art.694889
https://www.frontiersin.org/
Materia
IN VITRO MATURATION
CANINE
OXYGEN TENSION
INSULIN - TRANSFERRIN - SELENIUM
SOF MEDIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2021duquerodriguez1

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oai_identifier_str snrd:2021duquerodriguez1
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levelsDuque Rodríguez, MatteoCittadini, Camila O.Teplitz, Gabriela M.De Stefano, Adrián ArielLombardo, Daniel M.Salamone, Daniel FelipeIN VITRO MATURATIONCANINEOXYGEN TENSIONINSULIN - TRANSFERRIN - SELENIUMSOF MEDIUMFil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.tbls., grafs., fot.2021articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.3389/fcell.2021.694889issn:2296-634Xhttp://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1Frontiers in cell and developmental biologyVol.9art.694889https://www.frontiersin.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-10-16T09:28:47Zsnrd:2021duquerodriguez1instacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-10-16 09:28:48.627FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
title Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
spellingShingle Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
Duque Rodríguez, Matteo
IN VITRO MATURATION
CANINE
OXYGEN TENSION
INSULIN - TRANSFERRIN - SELENIUM
SOF MEDIUM
title_short Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
title_full Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
title_fullStr Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
title_full_unstemmed Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
title_sort Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
dc.creator.none.fl_str_mv Duque Rodríguez, Matteo
Cittadini, Camila O.
Teplitz, Gabriela M.
De Stefano, Adrián Ariel
Lombardo, Daniel M.
Salamone, Daniel Felipe
author Duque Rodríguez, Matteo
author_facet Duque Rodríguez, Matteo
Cittadini, Camila O.
Teplitz, Gabriela M.
De Stefano, Adrián Ariel
Lombardo, Daniel M.
Salamone, Daniel Felipe
author_role author
author2 Cittadini, Camila O.
Teplitz, Gabriela M.
De Stefano, Adrián Ariel
Lombardo, Daniel M.
Salamone, Daniel Felipe
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv IN VITRO MATURATION
CANINE
OXYGEN TENSION
INSULIN - TRANSFERRIN - SELENIUM
SOF MEDIUM
topic IN VITRO MATURATION
CANINE
OXYGEN TENSION
INSULIN - TRANSFERRIN - SELENIUM
SOF MEDIUM
dc.description.none.fl_txt_mv Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.
tbls., grafs., fot.
description Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.3389/fcell.2021.694889
issn:2296-634X
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1
identifier_str_mv doi:10.3389/fcell.2021.694889
issn:2296-634X
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in cell and developmental biology
Vol.9
art.694889
https://www.frontiersin.org/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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