Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels
- Autores
- Duque Rodríguez, Matteo; Cittadini, Camila O.; Teplitz, Gabriela M.; De Stefano, Adrián Ariel; Lombardo, Daniel M.; Salamone, Daniel Felipe
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.
Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.
Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.
tbls., grafs., fot. - Fuente
- Frontiers in cell and developmental biology
Vol.9
art.694889
https://www.frontiersin.org/ - Materia
-
IN VITRO MATURATION
CANINE
OXYGEN TENSION
INSULIN - TRANSFERRIN - SELENIUM
SOF MEDIUM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- acceso abierto
- Repositorio
- Institución
- Universidad de Buenos Aires. Facultad de Agronomía
- OAI Identificador
- snrd:2021duquerodriguez1
Ver los metadatos del registro completo
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Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levelsDuque Rodríguez, MatteoCittadini, Camila O.Teplitz, Gabriela M.De Stefano, Adrián ArielLombardo, Daniel M.Salamone, Daniel FelipeIN VITRO MATURATIONCANINEOXYGEN TENSIONINSULIN - TRANSFERRIN - SELENIUMSOF MEDIUMFil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia.Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina.Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina.Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.tbls., grafs., fot.2021articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.3389/fcell.2021.694889issn:2296-634Xhttp://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1Frontiers in cell and developmental biologyVol.9art.694889https://www.frontiersin.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-10-16T09:28:47Zsnrd:2021duquerodriguez1instacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-10-16 09:28:48.627FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse |
dc.title.none.fl_str_mv |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
title |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
spellingShingle |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels Duque Rodríguez, Matteo IN VITRO MATURATION CANINE OXYGEN TENSION INSULIN - TRANSFERRIN - SELENIUM SOF MEDIUM |
title_short |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
title_full |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
title_fullStr |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
title_full_unstemmed |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
title_sort |
Canine IVM with SOF medium, insulin - transferrin - selenium, and low O2 tension improves oocyte meiotic competence and decreases reactive oxygen species levels |
dc.creator.none.fl_str_mv |
Duque Rodríguez, Matteo Cittadini, Camila O. Teplitz, Gabriela M. De Stefano, Adrián Ariel Lombardo, Daniel M. Salamone, Daniel Felipe |
author |
Duque Rodríguez, Matteo |
author_facet |
Duque Rodríguez, Matteo Cittadini, Camila O. Teplitz, Gabriela M. De Stefano, Adrián Ariel Lombardo, Daniel M. Salamone, Daniel Felipe |
author_role |
author |
author2 |
Cittadini, Camila O. Teplitz, Gabriela M. De Stefano, Adrián Ariel Lombardo, Daniel M. Salamone, Daniel Felipe |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
IN VITRO MATURATION CANINE OXYGEN TENSION INSULIN - TRANSFERRIN - SELENIUM SOF MEDIUM |
topic |
IN VITRO MATURATION CANINE OXYGEN TENSION INSULIN - TRANSFERRIN - SELENIUM SOF MEDIUM |
dc.description.none.fl_txt_mv |
Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia. Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina. Fil: Duque Rodríguez, Matteo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina. Fil: Cittadini, Camila O. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina. Fil: Teplitz, Gabriela M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina. Fil: Teplitz, Gabriela M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina. Fil: De Stefano, Adrián Ariel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina. Fil: Lombardo, Daniel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología. Instituto de Investigación y Tecnología en Reproducción Animal. Buenos Aires, Argentina. Fil: Lombardo, Daniel M. CONICET - Universidad de Buenos Aires. Buenos Aires, Argentina. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Laboratorio Biotecnología Animal. Buenos Aires, Argentina. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal. Buenos Aires, Argentina. Assisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrinselenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF C BSA, SOF C BSA C FBS, and TCM-199 C FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF C BSA produced significantly higher degeneration rates compared to SOF C BSA C FBS (44 and 23%, respectively). Remarkably, supplementation with 1 ml/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM. tbls., grafs., fot. |
description |
Fil: Duque Rodríguez, Matteo. Politécnico Colombiano Jaime Isaza Cadavid. Facultad de Ciencias Agrarias. Medellin, Colombia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
article info:eu-repo/semantics/article publishedVersion info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
doi:10.3389/fcell.2021.694889 issn:2296-634X http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1 |
identifier_str_mv |
doi:10.3389/fcell.2021.694889 issn:2296-634X |
url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021duquerodriguez1 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
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openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
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application/pdf |
dc.source.none.fl_str_mv |
Frontiers in cell and developmental biology Vol.9 art.694889 https://www.frontiersin.org/ reponame:FAUBA Digital (UBA-FAUBA) instname:Universidad de Buenos Aires. Facultad de Agronomía |
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FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía |
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martino@agro.uba.ar;berasa@agro.uba.ar |
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