Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells

Autores
Blanco, Federico Carlos; Gravisaco, María José; Bigi, María Mercedes; García, Elizabeth Andrea; Marquez, Cecilia; McNeil, Mike; Jackson, Mary; Bigi, Fabiana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Blanco, Federico Carlos. CONICET. Buenos Aires, Argentina.
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Fil: García, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Marquez, Cecilia. High Technology Analytical Centre. Laboratory. Buenos Aires, Argentina.
Fil: McNeil, Mike. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.
Fil: Jackson, Mary. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, Fabiana. CONICET. Buenos Aires, Argentina.
Bovine tuberculosis is an important animal and zoonotic disease caused by Mycobacterium bovis. The innate immune response is the first line of defense against pathogens and is also crucial for the development of an efficient adaptive immune response. In this study we used an in vitro co-culture model of antigen presenting cells (APC) and autologous lymphocytes derived from peripheral blood mononuclear cells to identify the cell populations and immune mediators that participate in the development of an efficient innate response capable of controlling the intracellular replication of M. bovis. After M. bovis infection, bovine immune cell cultures displayed upregulated levels of iNOS, IL-22 and IFN-g and the induction of the innate immune response was dependent on the presence of differentiated APC. Among the analyzed M. bovis isolates, only a live virulent M. bovis isolate induced an efficient innate immune response, which was increased upon stimulation of cell co-cultures with the M. bovis culture supernatant. Moreover, we demonstrated that an allelic variation of the early secreted protein ESAT-6 (ESAT6 T63A) expressed in the virulent strain is involved in this increased innate immune response. These results highlight the relevance of the compounds secreted by live M. bovis as well as the variability among the assessed M. bovis strains to induce an efficient innate immune response.
grafs., tbls.
Fuente
Frontiers in Immunology
Vol.12
e674643
http://www.frontiersin.org/
Materia
INNATE IMMUNE
MYCOBACTERIUM BOVIS
NK CELLS
ESAT-6
BOVINE TUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2021blanco
Acceder
id FAUBA_a94fe9fb2f01062349082ba2d505ec9c
oai_identifier_str snrd:2021blanco
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cellsBlanco, Federico CarlosGravisaco, María JoséBigi, María MercedesGarcía, Elizabeth AndreaMarquez, CeciliaMcNeil, MikeJackson, MaryBigi, FabianaINNATE IMMUNEMYCOBACTERIUM BOVISNK CELLSESAT-6BOVINE TUBERCULOSISFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Blanco, Federico Carlos. CONICET. Buenos Aires, Argentina.Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.Fil: García, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Marquez, Cecilia. High Technology Analytical Centre. Laboratory. Buenos Aires, Argentina.Fil: McNeil, Mike. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.Fil: Jackson, Mary. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Bigi, Fabiana. CONICET. Buenos Aires, Argentina.Bovine tuberculosis is an important animal and zoonotic disease caused by Mycobacterium bovis. The innate immune response is the first line of defense against pathogens and is also crucial for the development of an efficient adaptive immune response. In this study we used an in vitro co-culture model of antigen presenting cells (APC) and autologous lymphocytes derived from peripheral blood mononuclear cells to identify the cell populations and immune mediators that participate in the development of an efficient innate response capable of controlling the intracellular replication of M. bovis. After M. bovis infection, bovine immune cell cultures displayed upregulated levels of iNOS, IL-22 and IFN-g and the induction of the innate immune response was dependent on the presence of differentiated APC. Among the analyzed M. bovis isolates, only a live virulent M. bovis isolate induced an efficient innate immune response, which was increased upon stimulation of cell co-cultures with the M. bovis culture supernatant. Moreover, we demonstrated that an allelic variation of the early secreted protein ESAT-6 (ESAT6 T63A) expressed in the virulent strain is involved in this increased innate immune response. These results highlight the relevance of the compounds secreted by live M. bovis as well as the variability among the assessed M. bovis strains to induce an efficient innate immune response.grafs., tbls.2021articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.3389/fimmu.2021.674643issn:1664-3224http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021blancoFrontiers in ImmunologyVol.12e674643http://www.frontiersin.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-10-16T09:28:35Zsnrd:2021blancoinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-10-16 09:28:36.527FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
title Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
spellingShingle Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
Blanco, Federico Carlos
INNATE IMMUNE
MYCOBACTERIUM BOVIS
NK CELLS
ESAT-6
BOVINE TUBERCULOSIS
title_short Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
title_full Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
title_fullStr Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
title_full_unstemmed Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
title_sort Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
dc.creator.none.fl_str_mv Blanco, Federico Carlos
Gravisaco, María José
Bigi, María Mercedes
García, Elizabeth Andrea
Marquez, Cecilia
McNeil, Mike
Jackson, Mary
Bigi, Fabiana
author Blanco, Federico Carlos
author_facet Blanco, Federico Carlos
Gravisaco, María José
Bigi, María Mercedes
García, Elizabeth Andrea
Marquez, Cecilia
McNeil, Mike
Jackson, Mary
Bigi, Fabiana
author_role author
author2 Gravisaco, María José
Bigi, María Mercedes
García, Elizabeth Andrea
Marquez, Cecilia
McNeil, Mike
Jackson, Mary
Bigi, Fabiana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INNATE IMMUNE
MYCOBACTERIUM BOVIS
NK CELLS
ESAT-6
BOVINE TUBERCULOSIS
topic INNATE IMMUNE
MYCOBACTERIUM BOVIS
NK CELLS
ESAT-6
BOVINE TUBERCULOSIS
dc.description.none.fl_txt_mv Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Blanco, Federico Carlos. CONICET. Buenos Aires, Argentina.
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Fil: García, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Marquez, Cecilia. High Technology Analytical Centre. Laboratory. Buenos Aires, Argentina.
Fil: McNeil, Mike. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.
Fil: Jackson, Mary. Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States.
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, Fabiana. CONICET. Buenos Aires, Argentina.
Bovine tuberculosis is an important animal and zoonotic disease caused by Mycobacterium bovis. The innate immune response is the first line of defense against pathogens and is also crucial for the development of an efficient adaptive immune response. In this study we used an in vitro co-culture model of antigen presenting cells (APC) and autologous lymphocytes derived from peripheral blood mononuclear cells to identify the cell populations and immune mediators that participate in the development of an efficient innate response capable of controlling the intracellular replication of M. bovis. After M. bovis infection, bovine immune cell cultures displayed upregulated levels of iNOS, IL-22 and IFN-g and the induction of the innate immune response was dependent on the presence of differentiated APC. Among the analyzed M. bovis isolates, only a live virulent M. bovis isolate induced an efficient innate immune response, which was increased upon stimulation of cell co-cultures with the M. bovis culture supernatant. Moreover, we demonstrated that an allelic variation of the early secreted protein ESAT-6 (ESAT6 T63A) expressed in the virulent strain is involved in this increased innate immune response. These results highlight the relevance of the compounds secreted by live M. bovis as well as the variability among the assessed M. bovis strains to induce an efficient innate immune response.
grafs., tbls.
description Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.3389/fimmu.2021.674643
issn:1664-3224
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021blanco
identifier_str_mv doi:10.3389/fimmu.2021.674643
issn:1664-3224
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021blanco
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in Immunology
Vol.12
e674643
http://www.frontiersin.org/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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score 12.712165

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